We narrowed to 5,570 results for: SPI

1. SNAP-ACE2

   Plasmid

   #178592

   Purpose

   Expresses SNAP-fused ACE2 protein that can be fluorescently labelled using SNAP substrates.

   Depositor

   Ralf Jockers

   Article

   Cecon et al Cell Chem Biol. 2021 Jul 2. pii: S2451-9

   Insert

   angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Flag and SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SARS-COV2-NSP1-HA-BASU

   Plasmid

   #154071

   Purpose

   Express SARS-CoV-2 NSP1 gene along with HA and BASU

   Depositor

   Paul Khavari

   Article

   Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

   Insert

   Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSYC-437

   Plasmid

   #163829

   Purpose

   Foxj1a:GCaMP6s. Fluorescent reporter for calcium signaling (GCaMP6s) driven by zebrafish foxj1a promoter.

   Depositor

   Seok-Yong Choi

   Article

   Zhang et al Nat Commun. 2020 Apr 20;11(1):1860. doi:

   Inserts

   Foxj1a Promoter
   GCaMP6s
   

   Use

   Multisite gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-mIRFP670nuc-TEV-Xph20-ETWV

   Plasmid

   #133044

   Purpose

   Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

   Insert

   Xph20-ETWV
   

   Use

   Tags

   NLS, TEV protease + TEV cleavage site, and miRFP6…

   Expression

   Mammalian

   Mutation

   S63K

   Promoter

   CAG

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMXs-WT-ATPIF1

   Plasmid

   #85403

   Purpose

   Retroviral vector expressing wild type human ATPIF1

   Depositor

   David Sabatini

   Article

   Chen et al Cell Rep. 2014 Apr 10;7(1):27-34. doi: 1

   Insert

   ATPIF1 (ATP5IF1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Wildtype

   Promoter

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pg-proPHD2-PHD2-EGFP

   Plasmid

   #87210

   Purpose

   PHD2-EGFP expressed under the control of the PHD2 promoter

   Depositor

   Violaine Sée

   Article

   Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

   Insert

   PHD2 (EGLN1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   H313N

   Promoter

   PHD2

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-MTS-KD-c-Src-FLAG

   Plasmid

   #44653

   Depositor

   Yoshimi Homma

   Article

   Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

   Insert

   v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
   

   Use

   Tags

   FLAG tag and mitochondria-targeting sequence (MTS…

   Expression

   Mammalian

   Mutation

   changed lysine 298 to methionine

   Promoter

   CMV promoter

   Available since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SBC009876

   Plasmid

   #226283

   Purpose

   Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

   Depositor

   Nigel Scrutton

   Article

   Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

   Inserts

   Coniferyl alcohol acyltransferase
   Eugenol synthase 1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGPN-ACE2

   Plasmid

   #187483

   Purpose

   Expression vector - ACE2

   Depositor

   Lewis Cantley

   Article

   Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

   Insert

   ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTriEX-Antenna-GDI Rac1

    Plasmid

    #83359

    Purpose

    Expression of Antenna-Rac1

    Depositor

    Klaus Hahn

    Article

    Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    FRET Antenna (mCerulean-cpVenus) and His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYPQ230-RVR

    Plasmid

    #108861

    Purpose

    LbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutations

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    LbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G548R, K554V and Y558R, Cpf1 is rice codon optimi…

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOPINO_H11

    Plasmid

    #184277

    Purpose

    Expresses nanobody H11 in E. coli

    Depositor

    Ray Owens

    Article

    Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Nanobody H11
    

    Use

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLX304-MARK3_T211A

    Plasmid

    #107236

    Purpose

    Lentiviral gene expression vector for phospho-MARK3_T221A expression

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MARK3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT2-CMV/TetO2-EYFP-GW

    Plasmid

    #153309

    Purpose

    Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Yellow Fluorescent Protein

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LLPS1-iLID::EGFP::FTH1 (pBS1042)

    Plasmid

    #185289

    Purpose

    For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    iLID::eGFP::FTH1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. px330-CIB1-dCas9

    Plasmid

    #63665

    Purpose

    Mammalian gRNA expression vector also expressing CIB1-dCas9

    Depositor

    Dong Kong

    Article

    Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and CMV

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-eYFP-FKBP-MAD1-AA

    Plasmid

    #114038

    Purpose

    Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

    Depositor

    Geert Kops

    Article

    Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

    Insert

    Human MAD1 (MAD1L1 Human)
    

    Use

    Tags

    eYFP-FKBP12

    Expression

    Mammalian

    Mutation

    MAD2 binding deficient MAD1 K541A/L543A

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM33_Orf9b

    Plasmid

    #156485

    Purpose

    Bacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Orf9b
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQE-80L 2xHis-LRRK2 Armadillo 1-552

    Plasmid

    #186017

    Purpose

    2xHis-LRRK2 Armadillo 1-552

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    2xHis-LRRK2 Armadillo 1-552 (LRRK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-eDHFR-Cdc42Q61L

    Plasmid

    #107267

    Purpose

    mCherry-eDHFR-Cdc42Q61L in pIVT vector for in vitro transcription. Note that Cdc42 in this plasmid keeps its CAAX domain.

    Depositor

    Michael Lampson

    Article

    Akera et al Science. 2017 Nov 3;358(6363):668-672. d

    Insert

    mCherry-eDHFR-Cdc42Q61L (CDC42, dhfr Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge