We narrowed to 11,766 results for: nsf

1. pAAV-U6-racRNA-A

   Plasmid

   #200824

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDmelOR-mRho.V5.mER.hOr56a

   Plasmid

   #126479

   Purpose

   Human codon-optimized D. melanogaster Orco (hOrco) and Or56a (hOr56a). Or56a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Inserts

   Or56a (Or56a Fly)
   Orco (Orco Fly)
   

   Tags

   H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

   Expression

   Mammalian

   Mutation

   codon optimization for H. sapiens and codon optim…

   Promoter

   CMV

   Available Since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. PGL4.11-COL1A1mSMAD

   Plasmid

   #230917

   Purpose

   Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.

   Depositor

   Avi Rosenberg

   Article

   Yang et al Lab Invest. 2024 May;104(5):100336. doi:

   Insert

   Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
   

   Use

   Luciferase

   Tags

   Luciferase-luc2p

   Expression

   Bacterial and Mammalian

   Mutation

   COL1A1 promoter with mutant potential SMAD bindin…

   Promoter

   COL1A1

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. TdLanYFP-AURKA-Aquamarine

   Plasmid

   #228562

   Purpose

   Encodes the AURKA biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/acceptor FRET pair and under the control of the CMV promoter.

   Depositor

   Giulia Bertolin

   Article

   Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

   Insert

   AURKA (AURKA, AURKA Human)
   

   Tags

   Aquamarine and TdLanYFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-U6-racRNA-B

   Plasmid

   #200825

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-U6-racRNA-C

   Plasmid

   #200826

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-U6-racRNA-D

   Plasmid

   #200827

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHIS-MDH1-HIS

   Plasmid

   #184558

   Purpose

   Bacterial expression of human MDH1 with HIS Tag

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Insert

   Malate dehydrogenase 1 (MDH1 Human)
   

   Tags

   6xHIS

   Expression

   Bacterial

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

   Plasmid

   #61359

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…

   Promoter

   CMV

   Available Since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid

    #61363

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PGL4.11-COL1A1mTEAD

    Plasmid

    #230916

    Purpose

    Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase- luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    mutant potential TEAD bind elements AGGAAT to CTG…

    Promoter

    COL1A1

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AURKA-Aquamarine

    Plasmid

    #228564

    Purpose

    Encodes a donor-only, Aquamarine-tagged version of the AURKA biosensor under the control of a CMV promoter

    Depositor

    Giulia Bertolin

    Article

    Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

    Insert

    AURKA (AURKA, AURKA Human)
    

    Tags

    Aquamarine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-dCas9-p300 Core (C1204R)

    Plasmid

    #61361

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation C1204R) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; C1204R mutation i…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid

    #61364

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDmelOR-mRho.V5.mER.hOr47a

    Plasmid

    #126478

    Purpose

    Human codon-optimized D. melanogaster Orco (hOrco) and Or47a (hOr47a). Or47a has N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved trafficking in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Inserts

    Or47a (Or47a Fly)
    Orco (Orco Fly)
    

    Tags

    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens and codon optim…

    Promoter

    CMV

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AiP5002 RabV-SADdeltaG-histone-EGFP

    Plasmid

    #176284

    Purpose

    Recombinant rabies virus expressing histone-EGFP

    Depositor

    Shenqin Yao, Allen Institute for Brain Science

    Article

    Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

    Insert

    H2B (H2BC11 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CCLMPC_HA-NUP98

    Plasmid

    #237482

    Purpose

    Express HA-tagged NUP98 in dual promoter lentiviral vector

    Depositor

    Charles Mullighan

    Article

    Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

    Insert

    HA-NUP98 (NUP98 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    MND/PGK

    Available Since

    June 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PGL4.11-COL1A1double mutants

    Plasmid

    #230918

    Purpose

    Double mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase-luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    COL1A1 promoter with mutant SMAD and TEAD binding…

    Promoter

    COL1A1 with mutant SMAD and TEAD elements

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEH_AAV_BRAF_ex15_V600E_S607S_1.8kb

    Plasmid

    #183078

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) BRAF V600E (T>A), S607S (TCC>AGT), 1.8kb homologous recombination donor template centered on BRAF exon 15 with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    BRAF Exon 15, 1.8kb homologous recombination donor, ~900 bp homology arms (BRAF Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    V600E (T>A), S607S (TCC>AGT)

    Promoter

    None for primary insert. PGK for puromycin resist…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

    Plasmid

    #183080

    Purpose

    rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

    Depositor

    Aviv Regev

    Article

    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

    Insert

    TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    

    Use

    AAV; Homologous recombination donor template

    Mutation

    -124C>T promoter, Exon 1 C7C (C21>T21) sile…

    Promoter

    None for primary insert (Though partial TERT prom…

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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