Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 7,670 results for: 11

Clear keywords
Showing: 4641 - 4660 of 7670 results

  2. pFrt-invCAG-ires-Luc

    Plasmid
    #63576
    Purpose
    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Stop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG
    Use
    Cre/Lox; Frt/flpe recombination mediated insertion
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    63576-plasmid-map-sequence-id-105724

    Enlarge

  5. STARR-seq luciferase INF enhancer vector_ORI_IFIT2

    Plasmid
    #99321
    Purpose
    Luciferase validation vector with IFIT2 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity
    Depositor
    Alexander Stark
    Article
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Insert
    enhancer; chr10: 91060605-91062447
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available Since
    Aug. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    99321-plasmid-map-sequence-id-190859

    Enlarge

  8. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

    Plasmid
    #140198
    Purpose
    Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    dLbCas12a(D156R)-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Mutation
    D832A DNase deactivated, D156R for improved activ…
    Promoter
    PgpdA
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140198-plasmid-map-sequence-id-309713

    Enlarge

  11. pODN-mNG-RHOA

    Plasmid
    #183836
    Purpose
    Repair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    RHOA homology arms with mNeonGreen-linker (RHOA Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-linker
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Available Since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183836-plasmid-map-sequence-id-362444

    Enlarge

  12. pcDNA3.1.H1-NP

    Plasmid
    #134367
    Purpose
    Nanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.
    Depositor
    Julien Hanson
    Article
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    H1-NP (HRH1 Human)
    Tags
    Flag and natural peptide of nanoluciferase
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134367-plasmid-map-sequence-id-306386

    Enlarge

Showing: 4641 - 4660 of 7670 results