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pUB-cHyPer2

Plasmid

#84728

Purpose

Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pSNAP-CLC-EYFP

Plasmid

#38011

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Insert

Clathrin, light polypeptide (LCa) (Clta Mouse)

Tags

EYFP and SNAP26m

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoB-ATP

Plasmid

#193819

Purpose

CMV overexpression of ChemoB-ATP in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-ATP

Expression

Mammalian

Mutation

EBFP2:N39Y, A206K; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z08

Plasmid

#120051

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mClover3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)

Plasmid

#179005

Purpose

Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)

Plasmid

#179004

Purpose

Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T HHARI RBR

Plasmid

#31254

Depositor

Rachel Klevit

Article

Wenzel et al Nature. 2011 Jun 2;474(7349):105-8. doi:

Insert

Human Homologue of Ariadne (HHARI) (ARIH1 Human)

Tags

GST

Expression

Bacterial

Mutation

RBR domain residues 177-395

Available Since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
PU6::klp-12_sgRNA

Plasmid

#46170

Purpose

klp-12 targeting sgRNA

Depositor

John Calarco

Article

Friedland et al Nat Methods. 2013 Jun 30. doi: 10.1038/n

Insert

klp-12 targeting sgRNA (klp-12 Synthetic)

Use

CRISPR

Expression

Worm

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

July 1, 2013

Availability

Academic Institutions and Nonprofits only

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CMV HA-MADD

Plasmid

#206150

Purpose

CMV HA-MADD

Depositor

Erika Holzbaur

Article

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

MADD (MADD Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagC shRNA #2

Plasmid

#26630

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagC shRNA (RRAGC Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pMaCTag-Z31

Plasmid

#120074

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with V5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

V5

Use

Pcr template

Tags

V5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P22

Plasmid

#120033

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z12

Plasmid

#120055

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet

Use

Pcr template

Tags

mScarlet

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pETM6-H10-mCherry

Plasmid

#66531

Purpose

Expresses mCherry under the control of Mutant T7 Promoter H10

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - H10

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-CDAmax-BlastR

Plasmid

#152995

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

CDA-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMaCTag-07

Plasmid

#124786

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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PT7-deGFP-MGapt

Plasmid

#67741

Purpose

T7 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T7term

Use

Synthetic Biology

Expression

Bacterial

Promoter

PT7

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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PCR4-beta-globin::lacZ-H11

Plasmid

#227000

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains human beta-globin minimal promoter)

Depositor

Guillaume Andrey

Article

Darbellay et al Nat Commun. 2024 Jun 6;15(1):4820. doi:

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

beta-globin

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P21

Plasmid

#120032

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with 24xSunTag

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

24xSunTag

Use

Pcr template

Tags

24xSunTag

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-Halo-10-PFN1-G118V

Plasmid

#204225

Purpose

Expression of Halo-Profilin-1(G118V) in tissue culture cells

Depositor

Jessica Henty-Ridilla

Article

Pimm et al Elife. 2022 Jun 6;11:e76485. doi: 10.755

Insert

PFN1 (PFN1 Human)

Tags

Halo, TEV site, SGLRSRAQAS linker

Expression

Mammalian

Mutation

G118V

Promoter

CMV

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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