We narrowed to 14,435 results for: NTS

1. pSF279

   Plasmid

   #167674

   Purpose

   Intermediate vector for five gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   attL1-ccdB-attL2
   

   Use

   CRISPR

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 190_SMAD4 in pmiRGlo

   Plasmid

   #78128

   Purpose

   luciferase reporter for miRNA activity

   Depositor

   Heidi Schwarzenbach

   Article

   Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

   Insert

   SMAD4 (SMAD4 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   3'UTR containing miRNA binding sites

   Promoter

   PGK

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. DCIP

   Plasmid

   #193860

   Purpose

   Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

   Depositor

   Markita Landry

   Article

   Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

   Insert

   NLS-mCherry-GFP1-10
   

   Tags

   SV40 NLS

   Expression

   Plant

   Promoter

   35S

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAGM4723::AtU6p::sgRNA2-2x35S-5′UTR::Cas9::NOST-AtU6p::sgRNA1

   Plasmid

   #49772

   Purpose

   Level 2 construct

   Depositor

   Sophien Kamoun

   Article

   Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

   Insert

   sgRNA_PDS2-Cas9-sgRNA_PDS1
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. His6-TEV-mCerulean3-pBluescript

   Plasmid

   #166757

   Purpose

   For purification of mCerulean3 fluorophore

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   mCerulean3
   

   Tags

   6x Histidine tag, and TEV protease site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOD_A1511

   Plasmid

   #91037

   Purpose

   Module A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4-P2A-TaCas9
   

   Use

   CRISPR

   Promoter

   OsAct1

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pTH1-SaSrtA

   Plasmid

   #64973

   Purpose

   Expresses the wild type of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59

   Promoter

   T7 promoter

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2T-CMV-AIDmax-BlastR

   Plasmid

   #152994

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   AID-nSpCas9-UGI-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHACK-GAL4>QF2(newHA1)

   Plasmid

   #104873

   Purpose

   HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   T2A-QF2
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac_human-IL-18

    Plasmid

    #214338

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p2T-CMV-ABEmaxCP1041-BlastR

    Plasmid

    #152990

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    ABE7.10-nSpCas9 CP1041
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAT15068-evoCBE

    Plasmid

    #163013

    Purpose

    plasmid expressing evoCBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    evoCBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. His6-TEV-Venus-pBluescript

    Plasmid

    #166756

    Purpose

    For purification of Venus fluorophore

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Venus
    

    Tags

    6x Histidine tag, and TEV protease site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEE391

    Plasmid

    #149281

    Purpose

    T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbPDS3sgRNA_FT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRU319

    Plasmid

    #167686

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::spCas9
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. UBQ10:sXVE:(MCS)-S11-DI-GFP1–9

    Plasmid

    #108260

    Purpose

    Binary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    UBQ10:sXVE:

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. ITPKA_3xmNeonGreen

    Plasmid

    #129606

    Purpose

    In vivo visualization of F-actin (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    F-tractin
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. p{CFD4-EYFP-3xP3::DsRed}

    Plasmid

    #86863

    Purpose

    Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2T-CMV-BE4maxCP1028-BlastR

    Plasmid

    #152993

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

    Insert

    BE4-nSpCas9 CP1028-UGI-UGI
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDR111_mannose_Bgal no NLS

    Plasmid

    #188399

    Purpose

    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Promoter

    D-mannose

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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