We narrowed to 24,626 results for: Nes

1. pSLIK TT 3xFLAG ID2 hygro

   Plasmid

   #98394

   Purpose

   Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   ID2 (ID2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLIK JunD-HA neo

   Plasmid

   #58515

   Purpose

   Lentiviral expression vector for an inducible HA-tagged mouse JunD

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   JunD (Jund Mouse)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pF713-pEGFP-N1-Hs-FL-METTL20 wt

   Plasmid

   #85114

   Purpose

   transfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusion

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human full-length METTL20 (METTL20 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. G-GECO1.2-Orai1Y80E

   Plasmid

   #73565

   Purpose

   Green fluorescent reporter of mutant Orai1Y80E-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

   Insert

   G-GECO1.2-Orai1Y80E (ORAI1 Synthetic, Human)
   

   Use

   Tags

   G-GECO1.2

   Expression

   Mammalian

   Mutation

   changed tyrosine 80 of Orai1 to glutamate

   Promoter

   CMV Immediate Early

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pF708-pET28a-Hs-delta38-METTL20-NHis wt

   Plasmid

   #85109

   Purpose

   expression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta38) METTL20 (METTL20 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX330-Flag-SpCas9-HF1 (without sgRNA)

   Plasmid

   #92102

   Purpose

   Expression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   Cbh

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEGB 35S:dCas9:Tnos (GB1191)

   Plasmid

   #68223

   Purpose

   Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed; human codon optimis…

   Promoter

   35S

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSLIK TT 3xFLAG ID2 neo

   Plasmid

   #98393

   Purpose

   Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   ID2 (ID2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TET-pLKO.1 PURO shID2 #1

   Plasmid

   #83086

   Purpose

   Lentiviral shRNA vector for inducible knockdown of human ID2

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shID2 (ID2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pBabe JunD-Venus puro

    Plasmid

    #58496

    Purpose

    mammalian expression of Venus-tagged mouse JunD

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JunD (Jund Mouse)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

11. mCardinal-H3-23

    Plasmid

    #56163

    Purpose

    Localization: Nucleus/Histones, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    H3 (H3C2 Human)
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pRC/CMV-TYK2K930R-FF-VSV

    Plasmid

    #139348

    Purpose

    Expression of kinase-dead TYK2-K930R missing the two tyrosines at the activation loop

    Depositor

    Sandra Pellegrini

    Article

    Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    V362F-K930R-Y1054F-Y1055F

    Promoter

    CMV

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRC/CMV-TYK21054-5FF-VSV

    Plasmid

    #139347

    Purpose

    Expression of TYK2 protein mutated in the two tyrosines (Y1054F/Y1055F) of the activation loop

    Depositor

    Sandra Pellegrini

    Article

    Gauzzi et al J Biol Chem. 1996 Aug 23;271(34):20494-5

    Insert

    TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V362F-Y1054F-Y1055F

    Promoter

    CMV

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSN-A112C-GpA-G83I

    Plasmid

    #191239

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA-G83I and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    

    Use

    Tags

    nuclease A from S. aureus fused to the TM domain …

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and Glycin…

    Promoter

    T7 promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. tet-pLKO.1_puro_shJUND #1

    Plasmid

    #136581

    Purpose

    Expresses an inducible short hairpin targeting human JUND sequence

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    shJUNDv1 (JUND Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CSK_HUMAN_D0

    Plasmid

    #79683

    Purpose

    This plasmid encodes the kinase domain of CSK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, Markus Seeliger, John Chodera

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSK (CSK Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCHKU38.2-2.2

    Plasmid

    #110670

    Purpose

    Expression of a fungal biosynthetic gene cluster

    Depositor

    Ronald Davis

    Article

    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

    Insert

    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-FLEXfrt-SaCas9-U6-sgGria3

    Plasmid

    #124869

    Purpose

    Mutagenesis of Gria3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria3 (Gria3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRD439

    Plasmid

    #168465

    Purpose

    For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS-mTurquoise2-H-NSdbd-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEN_TTmiRc2_BirA_CDKN1A

    Plasmid

    #136523

    Purpose

    Used as a donor vector to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Entry vector for gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge