We narrowed to 5,790 results for: SPI

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L2377-FRB-GPA(V84R)-20-114

Plasmid

#222898

Purpose

Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA(V84R)-20GS-NanoLuc114

Tags

Myc

Expression

Mammalian

Mutation

V84R in GPA

Promoter

CMV

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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L2085-WT-GPA-NLuc-Biosensor-Retrovirus-TD135

Plasmid

#222875

Purpose

Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with split NanoLuciferase reconstitution (11S/114 fragment). WT Glycophorin A (GPA) scaffold.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA(WT)-NanoLuc114-T2A-FKBP-GPA(WT)-NanoLuc11S

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

MSCV LTR

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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L2132-FKBP-GPA-114-5-CyOFP1

Plasmid

#222891

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc114-5GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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L2127-FRB-GPA-114-20-CyOFP1

Plasmid

#222886

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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L2129-FRB-GPA-114-5-CyOFP1

Plasmid

#222888

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114-5GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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L2128-FRB-GPA-114-10-CyOFP1

Plasmid

#222887

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114-10GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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L2131-FKBP-GPA-114-10-CyOFP1

Plasmid

#222890

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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L2130-FKBP-GPA-114-20-CyOFP1

Plasmid

#222889

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc114-20GS-CyOFP1

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-6xHis-TEV-ORF9c

Plasmid

#201023

Purpose

Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

open reading frame 9c

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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CB6-GFP-TAOK1 (K57A)

Plasmid

#197109

Purpose

Expression of kinase-deficient GFP-tagged TAOK1 (K57A) / PSK2 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

Insert

TAOK1 (K57A) (TAOK1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

CMV

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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W118-1_eGFP

Plasmid

#180379

Purpose

lentiviral transduction of eGFP gene

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

eGFP (eGFP )

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Dest-MBP-Nsp1

Plasmid

#191483

Purpose

Mammalian expression of MBP-tagged SARS-CoV-2 Nsp1

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

MBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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ACE2-IgFc probe

Plasmid

#184787

Purpose

Expression of His-tagged, soluble ACE2-IgFc microbody

Depositor

Ajit Chande

Article

Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

Insert

hACE2-IgFc (ACE2 Synthetic)

Tags

6xHis Tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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phABCA3-eGFP_CRISPR_Donor

Plasmid

#188540

Purpose

Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

Depositor

Darrell Kotton

Article

Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

Insert

EGFP

Use

CRISPR; Donor for homologous recombination

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-human-caspase-1/4a

Plasmid

#183393

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

CARD domain of human caspase-1 fused to catalytic domain of human caspase-4 (CASP1 Synthetic, Human)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pvLEA22mer_shuffle_3_mCh-2xFKBP (pBS1119)

Plasmid

#185314

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_3

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q19228_CAEEL_93-127 (pBS0735)

Plasmid

#185231

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_93-127. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_93-127

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOA4_ANDDA_21-367 (pBS0742)

Plasmid

#185234

Purpose

For the mammalian expression of the Chinese giant salamander protein APOA4_ANDDA_21-367. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOA4_ANDDA_21-367

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q7JLY3_CAEEL_48-124 (pBS0738)

Plasmid

#185232

Purpose

For the mammalian expression of the C. elegans protein Q7JLY3_CAEEL_48-124. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q7JLY3_CAEEL_48-124

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q19228_CAEEL_51-127_noLink (pBS0733)

Plasmid

#185229

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_51-127_noLink. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_51-127_noLink

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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