We narrowed to 13,725 results for: cas9

1. pAP546-2

   Plasmid

   #99476

   Purpose

   3XFlag::mNeptune::Myc

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   3XFlag::mNeptune::Myc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMLS271

   Plasmid

   #73722

   Purpose

   SapTrap SNAPtag + Cbr-unc-119 donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Snaptag + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAH233

   Plasmid

   #121144

   Purpose

   Expression of gRNA with BbsI placeholder for target cloning

   Depositor

   Katsunori Tanaka

   Article

   Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

   Insert

   gRNA cassette
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   S.pombe rrk1

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMLS268

   Plasmid

   #73726

   Purpose

   SapTrap SL2 C-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   SL2 (gdp-2/gdp-3 intergenic region)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAP693-3

   Plasmid

   #99492

   Purpose

   nirFP::3xFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   nirFP::3xFlag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTLR-donor

   Plasmid

   #118999

   Purpose

   Repair template for the TLR reporter

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   mVenus
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMLS254

   Plasmid

   #73721

   Purpose

   SapTrap Halotag + Cbr-unc-119 donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   Halotag + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMLS297

   Plasmid

   #73737

   Purpose

   SapTrap STOP codon + let-858 UTR N-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   STOP codon + let-858 3'-UTR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCfB8818

   Plasmid

   #126909

   Purpose

   Template for PCR amplification of HMG1from Yarrowia lipolytica

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   YlHMG1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMZ285

    Plasmid

    #52226

    Purpose

    Expression of sgRNA precursor targeted against ade6M210

    Depositor

    Mikel Zaratiegui

    Article

    Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

    Insert

    rrk1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    rrk1

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMLS269

    Plasmid

    #73727

    Purpose

    SapTrap SL2::SV40-NLS C-terminal connector donor plasmid

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    SL2 + SV40 NLS (gdp-2/gdp-3 intergenic region)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAP998-5

    Plasmid

    #99501

    Purpose

    3XFlag::tagBFP::linker::TEV

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    3XFlag::tagBFP::linker::TEV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LBJJ433

    Plasmid

    #117507

    Purpose

    AtEC1.2 promoter Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:GGAG:pEC1.2:AATG:BsaI
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAP576-2

    Plasmid

    #99481

    Purpose

    3XFlag::tagBFP::Myc

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Methods. 2017 May 15;121-122:86-93. doi:

    Insert

    3XFlag::tagBFP::Myc
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSF511

    Plasmid

    #222719

    Purpose

    CRISPR/Cas9 plasmid with gRNA1 for site gsdA, Cas9

    Depositor

    Matthias Steiger

    Article

    Matthias et al PLOS One

    Insert

    gRNA1(gsdA)
    

    Use

    CRISPR; A. niger

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pmbfA

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONOR 6colors

    Plasmid

    #229974

    Purpose

    bigMamAct assembled plasmid for 6colors cellular labelling

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psLIB TRE mTFP1-actin

    Plasmid

    #229970

    Purpose

    encodes a mTFP1-actin fusion construct under the control of a dox inducible promoter

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    mTFP1-actin
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psLIB TRE H2B iRFP720

    Plasmid

    #229968

    Purpose

    encodes a H2B-iRFP720 fusion contruct under the control of a dox inducible promoter

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Insert

    H2B iRFP720
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. psLIB U6

    Plasmid

    #229956

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psLIB EF1alpha

    Plasmid

    #229954

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge