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pmBanana-Hyg-CTCF-CtermNES-N1

Plasmid

#131851

Purpose

Expresses the [C-terminus of human CTCF (a.a. 577-728)]-mBanana with a nuclear export signal in mammalian cells

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

CTCF (CTCF Human)

Tags

mBanana

Expression

Mammalian

Mutation

the C-terminus of CTCF is linked to HIV-Rev prote…

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pQE30-HyPer7

Plasmid

#141071

Purpose

Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Hisx6

Expression

Bacterial

Promoter

T5

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro XLone-CCND2

Plasmid

#179845

Purpose

donor plasmid for inducible expression of CCND2 in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR, Luciferase, and TALEN ; Donor plasmid

Expression

Mammalian

Promoter

TRE3GS

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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iCas

Plasmid

#84232

Purpose

Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

Depositor

Meng How Tan

Article

Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

Insert

Cas9

Tags

2A-OFP co-expression and ERT2-ERT2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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MGAT2-RpHLuorin2

Plasmid

#171718

Purpose

Cis-/medial-Golgi expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMTS_mScarlet-i_N1

Plasmid

#85059

Purpose

In vivo visualization of the mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

MTS (COX8A Human)

Tags

mScarlet-i

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pBP Omomer

Plasmid

#113169

Purpose

Retroviral vector for expressing Omomer (tamoxifen inducible Omomyc), by DNA transfection or retroviral infection.

Depositor

Sergio Nasi

Article

Soucek et al Cancer Res. 2002 Jun 15;62(12):3507-10.

Insert

Omomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)

Use

Retroviral

Expression

Mammalian

Mutation

E57T, E64I, R70Q, R71N

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-HA-mScarlet3-tdP2A-scFv-GCN4-sfGFP-IRESv4-HaloTag-tdMCP

Plasmid

#241139

Purpose

Expresses Anti-HA frankenbody-mScarlet3, anti-GCN4 scfv-sfGFP, and HaloTag-tdMCP to track mature and nascent HA and SunTag-tagged proteins and MS2 tagged mRNA

Depositor

Tim Stasevich

Article

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-HA frankenbody
anti-GCN4 scfv
tdMCP

Tags

HaloTag, mScarlet3, and sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EF1a-Dio-ASAP5-Kv2.1-WPRE

Plasmid

#225708

Purpose

Cre dependent soma-targeted expression of the genetically encoded voltage indicator ASAP5

Depositor

Michael Lin

Article

Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

Has Service

AAV9

Insert

ASAP5-Kv2.1

Use

AAV

Promoter

EF1α

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-HaloSFPQY527A

Plasmid

#166949

Purpose

Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Use

Lentiviral

Tags

Halo

Mutation

SFPQ-Y527A

Promoter

eF1a

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pME-DreERT2 (JDW 1344)

Plasmid

#224523

Purpose

A Gateway-compatible middle entry clone containing Dre recombinase fused to mutant ERT2 for tamoxifen-inducible recombinase activity

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

DreERT2

Use

Gateway cloning

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-MS2V5-PABPC1

Plasmid

#65807

Purpose

expression of PABPC1-MS2 fusion. MS2 tethers PABPC1 to mRNAs containing MS2 stem loops.

Depositor

Niels Gehring

Article

Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

Insert

PAB1 (PABPC1 Human)

Tags

MS2 stem loops and V5

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-HA-mScarlet3-tdP2A-Frankenbody-FLAG-mEGFP-IRESv4-HaloTag-tdMCP

Plasmid

#241140

Purpose

Expresses Anti-HA frankenbody-mScarlet3, anti-FLAG frankenbody-mEGFP, and HaloTag-tdMCP to track mature and nascent HA and FLAG-tagged proteins and MS2-tagged mRNAand MS2-tagged mRNA

Depositor

Tim Stasevich

Article

Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi

Inserts

Anti-FLAG frankenbody
anti-FLAG frankenbody
tdMCP

Tags

HaloTag, mEGFP, and mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDonor-tBFP-NLS-Neo (Universal)

Plasmid

#80767

Purpose

Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

Insert

PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pFOS WT-GL3

Plasmid

#11983

Purpose

Reporter gene with mouse c-fos promoter. Induced by serum and many growth factors in quiescent cells.

Depositor

Ron Prywes

Article

Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

Insert

c-fos promoter (Fos Mouse)

Tags

Luc

Expression

Mammalian

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-α-COPI-WD40-Asp115Ala

Plasmid

#180760

Purpose

Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 mutant Asp115Ala (residues 1-327)

Depositor

Syed Saif Hasan

Article

Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

Insert

α-COPI-WD40-Asp115Ala

Tags

C-terminal strep tag

Expression

Mammalian

Mutation

Encodes residues 1-327; mutations: Asp115Ala, Leu…

Promoter

CMV

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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HA-HIF1alpha P402A/P564A-pBabe-puro

Plasmid

#19005

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 402 to Alanin…

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-GCaMP7f-CCR5TC

Plasmid

#216534

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to GCaMP7f, CCR5TC zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

GCaMP7f

Expression

Mammalian

Promoter

CAG

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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CMV Flag PAI-RBP

Plasmid

#45452

Depositor

Thomas Gelehrter

Article

Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

Insert

PAI-RBP (SERBP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pMTS_mScarlet_N1

Plasmid

#85057

Purpose

In vivo visualization of the mitochondria (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

MTS (COX8A Human)

Tags

mScarlet

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available Since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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