We narrowed to 11,735 results for: pcdna

1. pcDNA3-myc-p110α-CAAX

   Plasmid

   #39490

   Depositor

   Julian Downward

   Article

   Rodriguez-Viciana et al EMBO J. 1996 May 15;15(10):2442-51.

   Insert

   p110 (PIK3CA Bovine)
   

   Use

   Tags

   CAAX and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pcDNAI-GAL4-CREB-S142A

   Plasmid

   #46771

   Purpose

   Expression of GAL4 fusion to CREB-S142A

   Depositor

   Richard Maurer

   Article

   Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

   Insert

   GAL4(1-147)-CREB-S142A (Creb1, GAL4 Rat, Budding Yeast)
   

   Use

   Tags

   GAL4

   Expression

   Mammalian

   Mutation

   Changed CREB serine 142 to Alanine

   Promoter

   CMV

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-Flag-YOD1 C160S

   Plasmid

   #49785

   Purpose

   Express YOD1 C160S mutant with a N-terminal Flag-tag in mammalian cells

   Depositor

   Christian Schlieker

   Article

   Ernst et al Mol Cell. 2009 Oct 9;36(1):28-38. doi: 1

   Insert

   YOD1 (YOD1 Human)
   

   Use

   Tags

   Flag-tag

   Expression

   Mutation

   C160S

   Promoter

   Available since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

4. AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

   Plasmid

   #21800

   Depositor

   David Ron

   Article

   Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

   Insert

   PSMD14 (Psmd14 Mouse)
   

   Use

   Tags

   GST and TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1(+)Gαq-124-NLuc

   Plasmid

   #207153

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAQ (GNAQ Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position F(124)E

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

   Plasmid

   #207160

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNG2 (GNG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N-terminally tagged with LSS-mKATE2

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA 3.2/V5-DEST NET1A V5 WT

   Plasmid

   #69817

   Purpose

   Expresses NET1A-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   NET1A (NET1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA4-His-Max-A-PQBP1

   Plasmid

   #25802

   Depositor

   Marius Sudol

   Article

   Tapia et al J Biol Chem. 2010 Apr 21. ():.

   Insert

   PQBP1 (PQBP1 Human)
   

   Use

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.2/V5 hsa-mir-214

   Plasmid

   #26313

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   hsa-mir-214 (MIR214 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5/FRT/TO-globin_del5UTR_WT-xrRNA-4H

    Plasmid

    #108375

    Purpose

    FRT integration of wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

    Depositor

    Niels Gehring

    Article

    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

    Insert

    Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1(+)-RasAR(R89L)-NES

    Plasmid

    #205568

    Purpose

    RasAR negative-control mutant plasmid. Targeted to cytosol.

    Depositor

    Jin Zhang

    Article

    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

    Insert

    RasAR(R89L)-NES (RAF1 Human, Synthetic)
    

    Use

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Arg 89 mutated to Leu in Raf1 RBD region.

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA-FRT-TO-Nsp7-Nsp8_GC3opt

    Plasmid

    #157691

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp7-Nsp8_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. OeNL(Ca2+)_18μ-pcDNA3

    Plasmid

    #111928

    Purpose

    Orange color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    OeNL(Ca2+)_18μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-Dest47- WTN23C11orf83-GFP

    Plasmid

    #65845

    Purpose

    Mammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

    Depositor

    Amos Bairoch

    Article

    Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

    Insert

    Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WT Transmembrane

    Promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5-FRT/TO-Hygro-3xFLAG

    Plasmid

    #185359

    Purpose

    Vector backbone for mammalian expression of proteins with an N-terminal 3xFLAG tag.

    Depositor

    Ayaz Najafov

    Insert

    3xFLAG
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG

    Plasmid

    #127322

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EGFP Rab11a WT pcDNA3.1 Zeo (+)

    Plasmid

    #206079

    Purpose

    Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

    Depositor

    Annette Dolphin

    Article

    Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

    Insert

    RAB11A (RAB11A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1/Hygro-Mfn2-ActA

    Plasmid

    #191981

    Purpose

    Expression of Mfn2 tagged with mitochondrion localization signal (ActA)

    Depositor

    Luca Scorrano

    Insert

    Mfn2-ActA
    

    Use

    Tags

    ActA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA-f:PGC1(delta CTD)

    Plasmid

    #1030

    Depositor

    Bruce Spiegelman

    Article

    Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

    Insert

    PGC1a (Ppargc1a Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta CTD

    Promoter

    Available since

    Oct. 4, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO HIS DNAJB8

    Plasmid

    #19558

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 8 (DNAJB8 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge