164,577 results

1. pBabe-Puro-IKBalpha-mut (super repressor)

   Plasmid

   #15291

   Depositor

   William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   inhibitor of Kappa B alpha (NFKBIA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   S32A/S36A: “super-repressor” allele (resistant to…

   Promoter

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDB_182

   Plasmid

   #216103

   Purpose

   CRISPRi, EF1a-driven dCas9-KRAB (ZIM3) (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVTNT-T7-KPNA7

   Plasmid

   #26683

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 7 (KPNA7 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-mMaroon1

   Plasmid

   #83840

   Purpose

   Expresses mMaroon1

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mMaroon1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcdna3.1 hciap1

   Plasmid

   #8311

   Depositor

   Jon Ashwell

   Article

   Li et al Nature 2002 Mar 21;416(6878):345-7.

   Insert

   cIAP1 (BIRC2 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1-FLAG

   Plasmid

   #60360

   Purpose

   Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFR L858R

   Plasmid

   #11012

   Purpose

   Retroviral construct for expressing human EGFR with L858R mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR L858R (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   L858R (tumor-derived)

   Promoter

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-mem-TurboID

   Plasmid

   #149409

   Purpose

   Mammalian expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in mammalian expression plasmid. Amp/Neo selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Insert

   mem-TurboID
   

   Use

   Tags

   HA and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-P4M-SidM

   Plasmid

   #51471

   Purpose

   Biosensor for PtdIns4P

   Depositor

   Tamas Balla

   Article

   Hammond et al J Cell Biol. 2014 Apr 7.

   Insert

   P4M-SidM(546-647)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. SnailHA_pcDNA3

    Plasmid

    #31697

    Depositor

    Paul Wade

    Article

    Kajita et al Mol Cell Biol. 2004 Sep . 24(17):7559-66

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBE48

    Plasmid

    #174656

    Purpose

    CAPTURE method BAC origin of replication receiver plasmid

    Depositor

    Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV CEPIA3mt

    Plasmid

    #58219

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria (moderately low affinity variant)

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA3mt
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V E334D M339L

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX-RCC1-HA

    Plasmid

    #106938

    Purpose

    Transient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)

    Depositor

    Patrizia Lavia

    Article

    DeLuca et al Cancer Res. 2003 Mar 15;63(6):1430-7.

    Insert

    human RCC1-HA (RCC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. KARS_pNIC-Bio3

    Plasmid

    #153051

    Purpose

    Expression construct for producing Avi tagged Lysyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    KARS (KARS1 Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. TK4-NLS-EGFP

    Plasmid

    #239046

    Purpose

    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

    Depositor

    Marius Wernig

    Inserts

    EGFP
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2 and c-myc-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBABE-hygro-hTERT

    Plasmid

    #1773

    Purpose

    Retroviral expression of hTERT. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFUGW SpCas9

    Plasmid

    #131506

    Purpose

    lentiviral expression of SpCas9 under hUBC promotor

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    SpCas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hUBC

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

    Plasmid

    #133028

    Purpose

    Expresses EGFP-tagged Golgi apparatus marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Golgin84 (GOLGA5 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-673

    Promoter

    Human elongation factor 1 alpha

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tandem p40p35

    Plasmid

    #108665

    Purpose

    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker

    Depositor

    Nevil Singh

    Insert

    interleukin-12 (Il12a, Il12b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    IL-12 dimers have a Gly-Ser linker

    Promoter

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. NW9

    Plasmid

    #133442

    Purpose

    Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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