We narrowed to 30,195 results for: REP
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Plasmid#197090PurposePiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiationDepositorAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only
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pCAGGS-4EBP1 F113A
Plasmid#81122Purposeexpresses a constitutively active mutant of 4E-BP1 under the CAG promoterDepositorAvailable SinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS170-enNTS1
Plasmid#199152PurposeJZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)DepositorInsertenNTS1 (Ntsr1 Rat)
Tags10xHis tag, Avi tag, MBP, and muGFPExpressionBacterialMutationV208M, C273S, C391S and C393S (over NTS1-B5)PromoterLac/T5Available SinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A
Plasmid#154773Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
TagsFLAGExpressionMammalianMutationY125A in G1PylS, hybrid PylT with A41AA and C55A …PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE
Plasmid#139087PurposeExpresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.DepositorInsertSpCas9-NG
UseCRISPR and LentiviralExpressionMammalianMutationL1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-shRNA
Plasmid#82697PurposeFor targeting shRNA construct into human AAVS1 locus, using genome editing. Expresses shRNA of interest (cloning: EcoRI and AgeI) under U6 promoter, flanked by AAVS1 homology arms.DepositorTypeEmpty backboneUseRNAiPromoterhPGK, U6Available SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/20]
Plasmid#206744PurposeMammalian Expression of GFP scFV. Derived from hybridoma N86/20 scFv.DepositorInsertGFP (Aequorea victoria) recombinant scFV
ExpressionMammalianPromoterCMVAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAT-PB-CD2APtk
Plasmid#86004Purposehomology donor for AAT locus with piggyBac-flanked DsRedEx_T2A_puroTK cassetteDepositorInsertDsRedEx_T2A_puro_dTK
UsePiggybac exciseableExpressionMammalianPromoterCAGAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHLSec2-irisin-his
Plasmid#122729PurposeExpresses in mammalian cells (HEK293):glycosylated and secreted irisin-hisDepositorInsertirisin (ectodomain of FNDC5) (Fndc5 Mouse, Bovine, Human, Rat)
Tagshis and signal sequence for secretionExpressionMammalianAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgA1/κ
Plasmid#61888PurposeExpresses HER2/neu receptor specific humanized IgA1/κ antibody isotype (Trastuzumab-IgA1/κ)DepositorInsertsHER2/neu receptor specific humanized Alpha 1 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
ExpressionMammalianPromotermEF1 Prom and rEF1 PromAvailable SinceMarch 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-LRP6-3XFlag
Plasmid#180144Purposeflag tagged LRP6DepositorAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/44]
Plasmid#206743PurposeMammalian Expression of GFP scFV. Derived from hybridoma N86/44 scFv.DepositorInsertGFP (Aequorea victoria) recombinant scFV
ExpressionMammalianPromoterCMVAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sox2-CreERT2
Plasmid#135618PurposeDonor vector for genomic targeting of a P2A-CreERT2 cassette to the 3' end of the mouse Sox2 locusDepositorInsertP2A-CreERT2 flanked by Sox2 homology arms (Sox2 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsP2A-CreERT2Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2 LRP6-eGFP
Plasmid#180143Purposeto make movies of LRP6 vesiclesDepositorAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_M18
Plasmid#82356PurposeExpression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertmouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)
TagsnoneExpressionMammalianMutationnonePromoterhEF1-HTLV promAvailable SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-Trastuzumab-IgG4/κ
Plasmid#61887PurposeExpresses HER2/neu receptor specific humanized IgG4/κ antibody isotype (Trastuzumab-IgG4/κ)DepositorInsertsHER2/neu receptor specific humanized Gamma 4 heavy chain expression cassette
HER2/neu receptor specific humanized kappa light chain expression cassette
ExpressionMammalianPromotermEF1 Prom and rEF1 PromAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-R26-FLEX-mStr
Plasmid#135619PurposeDonor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locusDepositorInsertCAG-FLEX-mStrawberry flanked by Rosa26 homology arms (Gt(ROSA)26Sor Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNTI194
Plasmid#84266PurposeExpresses Avi-tagged RPL1 and humanized birADepositorInsertsTagsAvi tag, Flag, Gly/Ser linker, and TEV siteExpressionMammalianMutationR33A, A34E (*see below)PromoterCMV and P2A peptideAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/8]
Plasmid#199429PurposeMammalian expression of GFP scFv. Derived from hybridoma N86/8.DepositorInsertrecombinant mouse scFv targetingGFP (Aequorea victoria)
ExpressionMammalianPromoterCMVAvailable SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-M80-F2-IgG1/κ
Plasmid#50383PurposeExpresses human IgG1/κ antibody isotype with unknown specificity. Useful vector for cloning of human antibodies with kappa light chainDepositorInsertshuman Gamma 1 heavy chain expression cassette with unknown specificity
human Kappa light chain expression cassette with unknown specificity
ExpressionMammalianPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available SinceJan. 27, 2014AvailabilityAcademic Institutions and Nonprofits only