We narrowed to 12,952 results for: sequence

1. pUAST mito roGFP2-Orp1

   Plasmid

   #64997

   Purpose

   for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Tags

   roGFP2

   Expression

   Insect

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available Since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. SERA1-bio-His

   Plasmid

   #50812

   Purpose

   Expresses enzymatically monobiotinylated full length SERA1

   Depositor

   Gavin Wright

   Article

   Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

   Insert

   SERA1
   

   Use

   Tags

   ratCD4d3+4,biotinylation sequence, 6xHis

   Expression

   Mammalian

   Mutation

   all potential N-linked glycosylation sites (N-X-S…

   Promoter

   CMV

   Available Since

   Feb. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. phNFL-Q332P

   Plasmid

   #132599

   Purpose

   Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E Q332P mutation

   Depositor

   Anthony Brown

   Article

   Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

   Insert

   Nefl (Nefl Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   995A>C;Gln332Pro

   Promoter

   CMV

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHBS1410 GFP-TDP43 F-S

   Plasmid

   #118799

   Purpose

   To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   All F to S in CTD of full-length TDP43

   Promoter

   Available Since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. phNFL-E396K

   Plasmid

   #132595

   Purpose

   Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E E396K mutation

   Depositor

   Anthony Brown

   Article

   Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

   Insert

   NEFL (NEFL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   1186G>A; Glu396Lys

   Promoter

   CMV

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVC-CRISPR

   Plasmid

   #120369

   Purpose

   Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

   Depositor

   Jennifer Doudna

   Article

   Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

   Inserts

   CRISPR1
   CRISPR2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pmalc4x_I499F_TEV

   Plasmid

   #47703

   Purpose

   Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation I499F

   Depositor

   Raquel Lieberman

   Article

   Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

   Insert

   Olfactomedin-like domain with I499F mutation (MYOC Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   changed I499F

   Promoter

   Ptac

   Available Since

   Sept. 9, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. 459 pME-etv2

   Plasmid

   #49002

   Purpose

   full length coding sequence for zebrafish etv2 gene in gateway middle entry vector

   Depositor

   Nathan Lawson

   Article

   Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

   Insert

   ets variant gene 2 (etsrp Zebrafish)
   

   Use

   Gateway middle entry

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. attB-P7-attP-YFP

   Plasmid

   #195571

   Purpose

   A strong P7 promoter flanked by Bxb1 recombination sites attB and attP. Downstream of attP is a strong RBS and YFP coding sequence.

   Depositor

   Richard Murray

   Article

   Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

   Insert

   Promoter flanked by Bxb1 recombination sites attB and attP with YFP downstream
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   P7

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLBC0010

    Plasmid

    #172774

    Purpose

    Level 0 module containing NT1_CTPSSU

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLBC0011

    Plasmid

    #172775

    Purpose

    Level 0 module containing NT2_CTPSSU

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-GST-Q11

    Plasmid

    #185790

    Purpose

    Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    GST-Q11
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. IRE-nonOpt-TREAT

    Plasmid

    #196922

    Purpose

    The codon optimized Renilla coding sequence in the IRE-TREAT reporter was replaced by non-optimal Renilla containing 93 synonymous codon substitutions.

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    non-optimal Renilla luciferase
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. myc-Rapr-FAK-YM

    Plasmid

    #25927

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

    Insert

    RapR-FAK (Ptk2 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    This myc-RapR-FAK-YM plasmid contains FAK mutatio…

    Promoter

    Available Since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pSLIK 3XFLAG-MKK4-EE-NLS hygro

    Plasmid

    #87779

    Purpose

    Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK4
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

16. secCitrine

    Plasmid

    #181964

    Purpose

    Basic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    signal peptide sequence from basic chitinase
    

    Use

    Tags

    Citrine YFP

    Expression

    Plant

    Mutation

    Promoter

    1.5 kb MUM4 promoter

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-BCA-Pep-H

    Plasmid

    #185782

    Purpose

    Encodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    BCA-Pep-H
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMpGWB231

    Plasmid

    #68622

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Emerald Luc fused with PEST sequence [ELuc(PEST)]

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3_4xUAS_CP-candidate_luc+

    Plasmid

    #125154

    Purpose

    STAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    4 x upstream activating sequence (UAS)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MD~E-pCLBabe

    Plasmid

    #20912

    Depositor

    Stephen Tapscott

    Article

    Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

    Insert

    MyoD~E12 forced dimer
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Short flexible linker region between the MyoD and…

    Promoter

    Available Since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

21. SERA5-bio-His

    Plasmid

    #50817

    Purpose

    Expresses enzymatically monobiotinylated full length SERA5

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    SERA5
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pOmpT

    Plasmid

    #112123

    Purpose

    Bacterial expression outer membrane protein T (OmpT), wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    OmpT (ompT E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHBS1396 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT]

    Plasmid

    #118804

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHBS1412 GFP-TDP43 FYW-L

    Plasmid

    #118801

    Purpose

    To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    All FYW to L in CTD of full-length TDP43

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Or30a-Gal4

    Plasmid

    #63027

    Purpose

    To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or30a, using the promoter sequences of the gene cloned 5' of Gal4

    Depositor

    Leslie Vosshall

    Article

    Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

    Insert

    Drosophila melanogaster Or30a promoter
    

    Use

    Tags

    Drosophila ADH (aa's 1-18) fused to Yeast Ga…

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

26. phNFL-P440L

    Plasmid

    #132596

    Purpose

    Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E P440L mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    NEFL (NEFL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1319C>T; Pro440Leu

    Promoter

    CMV

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SERA9-bio-His

    Plasmid

    #50820

    Purpose

    Expresses enzymatically monobiotinylated full length SERA9

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    SERA9
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOmpW

    Plasmid

    #112121

    Purpose

    Bacterial expression of outer membrane protein W (OmpW), wild type

    Depositor

    Karen Fleming

    Article

    Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

    Insert

    OmpW (ompW E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminal signal sequence not included and repla…

    Promoter

    T7

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MSRP5-bio-His

    Plasmid

    #50805

    Purpose

    Expresses enzymatically monobiotinylated full length MSRP5

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    MSRP5
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. UASGAL-PCYC

    Plasmid

    #60335

    Purpose

    Contains a promoter of activating sequence derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge