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EGFP-mOrange pHis-//

Plasmid

#53302

Purpose

Expresses His-tagged EGFP-mOrange fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Insert

EGFP

Tags

His and mOrange

Expression

Bacterial

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAK

Plasmid

#19490

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

dnaK molecular chaperone DnaK

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pEc-crRNA1

Plasmid

#170088

Purpose

encodes E. coli type I-E single spacer array

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E single spacer array

Use

CRISPR and Synthetic Biology

Promoter

J23119

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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iXFGFR1

Plasmid

#31257

Depositor

Masahiro Yamagishi

Article

Yamagishi et al Int J Dev Biol. 2010 . 54(1):93-104.

Insert

Xenopus FGFR1-ICD-Fv’Fv (fgfr1-a Frog)

Tags

HA epitope tag and myristoylation signal

Expression

Bacterial and Mammalian

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pRibo-APEX2

Plasmid

#111698

Purpose

Theophylline-inducible expression of APEX2 from a mycobacterial shuttle plasmid (episomal)

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Tags

V5

Expression

Bacterial

Promoter

hsp60-ribo

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pPD540 Reporter Template v3

Plasmid

#138750

Purpose

Template for COMET promoters, expresses EYFP reporter upon promoter activation. BsaI sites produce non-palindromic sticky ends.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-2

Plasmid

#87333

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0058

Plasmid

#117539

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

sgRNA (SaCas9)

Expression

Plant

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-bantam

Plasmid

#22788

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

bantam (ban primary transcript, Fly)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-mir-12

Plasmid

#22760

Depositor

Elisa Izaurralde

Article

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Insert

micro RNA 12 (mir-12 primary transcript, Fly)

Use

Drosophila expression

Available Since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pPD069 ZF43x6-Spaced_EYFP

Plasmid

#138879

Purpose

EYFP reporter vector for the ZF43x6-Spaced promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Spaced

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Spaced

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMMB206-CT61

Plasmid

#165410

Purpose

Bacteria gene circuit on low copy backbone.

Depositor

Xiaojun Tian

Article

Zhang et al Nat Commun. 2021 Feb 8;12(1):853. doi: 1

Insert

CT61 gene circuit

Expression

Bacterial

Available Since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pUCBB-lacZa

Plasmid

#32563

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

LacZ-alpha

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pFastBac dRING1F

Plasmid

#1928

Depositor

Robert Kingston

Article

Francis et al Mol Cell 2001 Sep;8(3):545-56.

Insert

dRING1 (Sce Fly)

Tags

flag

Expression

Insect

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
EZH2-TALEN_Left_No_FLAG

Plasmid

#79899

Purpose

Untagged EZH2-TALEN_Left to target the N-terminus of human EZH2

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

EZH2-TALEN_Left_No_FLAG

Use

TALEN

Expression

Mammalian

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnn1a

Plasmid

#164956

Purpose

Zebrafish kcnn1a gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn1a (kcnn1a Zebrafish)

Use

Pcr cloning vector

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBRIT Pax7 N281

Plasmid

#17524

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 281 amino acids

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
p6666 MSCV-IP N-HAonly 18E6

Plasmid

#44137

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV18 E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
3xDHFR-myc

Plasmid

#20216

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xDHFR (folA E. coli)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pAGH23

Plasmid

#107262

Purpose

contains CurT-mTurquoise2 construct used to replace the native CurT in Synechocystis PCC 6803 via homologous recombination.

Depositor

Erin O'Shea

Article

Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

Insert

curT (slr0483 )

Use

Synthetic Biology

Tags

mTurquoise2

Expression

Bacterial

Promoter

native CurT promoter

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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