165,343 results

1. pBabe-Kras G12C

   Plasmid

   #58901

   Purpose

   Expresses human Kras with a G12C mutation

   Depositor

   Channing Der

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G12C mutation

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-Lifeact-tdTomato

   Plasmid

   #64048

   Purpose

   Fluorescent reporter for F-actin labeling in living cells

   Depositor

   Weiping Han

   Article

   Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

   Insert

   Lifeact
   

   Use

   Lentiviral

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAct:dCas9-VPR

   Plasmid

   #78898

   Purpose

   Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   pActin (Drosophila)

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. SV-ABAactDA

   Plasmid

   #38247

   Depositor

   Jerry Crabtree

   Article

   Liang et al Sci Signal. 2011 Mar 15;4(164):rs2.

   Inserts

   VP16/PYL1
   Gal4DBD/ABI*
   

   Tags

   Flag, Gal4DBD, HA, and VP16

   Expression

   Mammalian

   Mutation

   D143A

   Promoter

   SV40 (IRES) and SV40p

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. sortase A pentamutant (eSrtA) in pET29

   Plasmid

   #75144

   Purpose

   evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

   Depositor

   David Liu

   Article

   Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

   Insert

   sortase A pentamutant
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T. Deleted amino a…

   Promoter

   T7

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. hGli3 flag3x

   Plasmid

   #84921

   Purpose

   mammalian expression of Gli3

   Depositor

   Martin Fernandez-Zapico

   Insert

   Gli3 (GLI3 Human)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TO-hNGN2

   Plasmid

   #172115

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   hNGN2 (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACEBac1-PARP1-Flag-His-WT

   Plasmid

   #111572

   Purpose

   Expresses full length PARP1 in Sf9 (insect) cells

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   PARP1 (PARP1 Human)
   

   Tags

   C-terminal 6xHis and C-terminal Flag

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-mSmo

   Plasmid

   #25395

   Depositor

   Philip Beachy

   Article

   Chen et al Genes Dev. 2002 Nov 1. 16(21):2743-8.

   Insert

   mouse Smoothened cDNA (Smo Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIJ8

    Plasmid

    #68122

    Purpose

    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Inserts

    flp recombinase
    rhaS
    rhaR
    

    Expression

    Bacterial

    Promoter

    rhamnose

    Available Since

    Dec. 8, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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