164,629 results

1. pcDNA3 RLUC POLIRES FLUC

   Plasmid

   #45642

   Purpose

   Bicistronic reporter plasmid expressing Renilla luciferase and firefly luciferase in mammalian cells.

   Depositor

   Nahum Sonenberg

   Article

   Poulin et al J Biol Chem. 1998 May 29;273(22):14002-7

   Insert

   RLUC - IRES - FLUC
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRE-Fast-FT

   Plasmid

   #31913

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

   Insert

   Fast-FT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M18V/E34K/K69R/L84W/S149T/A224S compared to mCher…

   Promoter

   PTight Tet-responsive PminCMVd

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-Daxx/pRK5

   Plasmid

   #52023

   Purpose

   Expresses HA-Daxx in mammalian cells

   Depositor

   Xiaolu Yang

   Article

   Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

   Insert

   HA-Daxx (DAXX Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINDUCER21-RORA

   Plasmid

   #51303

   Purpose

   Expression of RORA after doxycycline administration

   Depositor

   George Daley

   Article

   Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

   Insert

   RAR-related orphan receptor A (RORA Human)
   

   Use

   Lentiviral

   Tags

   n/a

   Expression

   Mutation

   full length ORF

   Promoter

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR SFFVp YFP-PIF-SOS2cat

   Plasmid

   #50851

   Purpose

   Optogenetic Ras activation system; cytoplasmic component

   Depositor

   Wendell Lim

   Article

   Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

   Inserts

   SOS2cat (SOS2 Human)
   PIF6 1-100
   

   Use

   Lentiviral

   Tags

   YFP

   Expression

   Mutation

   residues 566–1049

   Promoter

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-SynI-CreOn-FlpOff-TeNT-P2A-EGFP

   Plasmid

   #176282

   Purpose

   Viral vector for co-expression of TeNT and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   TeNT-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin I

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. cacna2d1 pcDNA3

   Plasmid

   #206103

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit

   Depositor

   Annette Dolphin

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCherry.90 beta

   Plasmid

   #108223

   Purpose

   Mammalian expression vector for mCherry fusion to human Hsp90 beta

   Depositor

   Didier Picard

   Article

   Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

   Insert

   mCherry-Hsp90b (HSP90AB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0043-PspCas13b-gRNA non target

   Plasmid

   #223698

   Purpose

   gRNA control for dPspCas13b-FTO

   Depositor

   Chuan He

   Article

   Wei et al Science. 2022 May 27;376(6596):968-973.

   Insert

   gRNA target sequence for luciferase control
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. HR_G3BP1-V5-APEX2-GFP

    Plasmid

    #105284

    Purpose

    homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

    Depositor

    Eugene Yeo

    Article

    Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

    Insert

    G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)
    

    Use

    Human homologous recombination donor

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCXLE-hMLN

    Plasmid

    #27079

    Purpose

    Non-integrating (episomal) expression of human C-MYC, LIN28 and NANOG

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    C-MYC, LIN28, NANOG (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-AURKB

    Plasmid

    #23682

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    AURKB (AURKB Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pREDawn-AmpR-MCS

    Plasmid

    #188978

    Purpose

    For red light-induced bacterial expression of gene of interest (AmpR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD-pHuji

    Plasmid

    #61555

    Purpose

    Red fluorescent protein based pH indicator in bacterial expression vector

    Depositor

    Robert Campbell

    Article

    Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

    Insert

    pHuji
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

15. LifeAct-mGarnet2

    Plasmid

    #104308

    Purpose

    codes for a fusion of fluorescent protein mGarnet2 and lifeact that targets F-actin in live cells

    Depositor

    Ulrich Nienhaus

    Article

    Matela et al Chem Commun (Camb). 2017 Jan 10;53(5):97

    Insert

    LifeAct-mGarnet2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. ampl43

    Plasmid

    #161830

    Purpose

    pRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.

    Depositor

    Saeed Tavazoie

    Article

    Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-oScarlet

    Plasmid

    #137135

    Purpose

    Viral expression of oScarlet

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    oScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    E95D

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG426-GAL-Alpha-synuclein

    Plasmid

    #181715

    Purpose

    Galactose inducible expression of Alpha-synuclein

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pN1-QUE37C

    Plasmid

    #129318

    Purpose

    ATP measurement

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG_smFP FLAG

    Plasmid

    #59756

    Purpose

    encodes multiple copies of an epitope tag FLAG

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    smFP _FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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