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AiP15072 - pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA (Alias: CN5072)

Plasmid

#230402

Purpose

AiE0680m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV2A

Plasmid

#224440

Purpose

Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDQTTL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-NUP188

Plasmid

#159893

Purpose

Expresses NUP188 in mammalian cells

Depositor

Kozo Tanaka

Article

Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:

Insert

NUP188 (NUP188 Human)

Tags

EGFP

Expression

Mammalian

Mutation

To avoid NotI site, first three amino acids are d…

Promoter

CMV

Available Since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pET-MCN-10His-mStayGold (E138D)

Plasmid

#211361

Purpose

Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

mStayGold

Tags

10xHis-tag

Expression

Bacterial

Mutation

E138D

Promoter

T7

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI-2P-WPRE

Plasmid

#179464

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV9

Insert

JEDI-2P

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Human RNF149-3X FLAG

Plasmid

#225332

Purpose

Lentiviral expression of human RNF149 with 3x FLAG tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF149 (RNF149 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA

Plasmid

#216319

Purpose

Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cerulean + splice acceptor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA

Plasmid

#216318

Purpose

Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

split cerulean fluorescent protein + splice donor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28 His6-mEGFP-D4

Plasmid

#226372

Purpose

Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

Depositor

Catherine Tomasetto

Article

Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

Insert

D4 (pfoA Clostridium perfringens)

Tags

His6 and monomeric EGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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L1-neo-TET

Plasmid

#51284

Purpose

expresses codon optimized human L1 retrotransposon driven by CMV promoter and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

L1RE1 (L1RE1 Human)

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-GPHN-FingR-mGold2s-CCR5TC

Plasmid

#231785

Purpose

Visualization of inhibitory post-synapse using GPHN-FingR-mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

GPHN-FingR-mGold2s-CCR5TC

Expression

Mammalian

Promoter

Actin promoter with CMV enhancer

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pJMP3664

Plasmid

#222350

Purpose

Expression vector with PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Type

Empty backbone

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCW empty vector

Plasmid

#184708

Purpose

Lentiviral empty vector for doxycycline-inducible expression

Depositor

Alessia Ciarrocchi, Gloria Manzotti

Article

Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7

Plasmid

#62276

Purpose

mammalian expression of human Alpha7 (CHRNA7)

Depositor

Sherry Leonard, Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRNA7 (CHRNA7 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-IRES-NRASG12D-BFP

Plasmid

#236202

Purpose

Expresses NRASG12D in mammalian cells

Depositor

Michael Heuser

Article

Mohanty et al Blood Adv. 2025 May 7:bloodadvances.2024

Insert

NRAS (NRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12D

Promoter

MSCV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-pp65-b2m-HLA-A0201

Plasmid

#196511

Purpose

Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCE-hOCT3/4

Plasmid

#41813

Purpose

Non-integrating (episomal) expression of human OCT3/4

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

POU5F1 (POU5F1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2-sgCTL

Plasmid

#234771

Purpose

Non Targeting Control

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Non Targeting Control sgRNA

Use

Lentiviral

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pKIKOarsBCm

Plasmid

#46763

Purpose

E.coli genomic integration at arsB

Depositor

Lars Nielsen, Claudia Vickers

Article

Sabri et al Microb Cell Fact. 2013 Jun 24;12(1):60.

Type

Empty backbone

Use

E.coli genomic integration

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl2

Plasmid

#17999

Depositor

Clark Distelhorst

Article

Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

Insert

Bcl-2 (BCL2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 4, 2008

Availability

Academic Institutions and Nonprofits only

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