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We narrowed to 9,621 results for: bli

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Showing: 7111 - 7140 of 9621 results

  1. huntingtin_V2095-V3138_NbioCflag

    Plasmid
    #194765
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV and P10
    Available Since
    July 26, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194765-plasmid-map-sequence-id-402569

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  2. pUASp YFP Rac1 DN

    Plasmid
    #52234
    Purpose
    Drosophila expression of YFP-Rac1 dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    T17N; DN mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2

    Plasmid
    #75295
    Purpose
    An AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.
    Depositor
    Brandon Harvey
    Insert
    eGFP-2A-mKv1.2 (Kcna2 Synthetic, Mouse)
    Use
    AAV
    Promoter
    human SYN1
    Available Since
    Jan. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    75295-plasmid-map-sequence-id-142411

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  4. pExo1

    Plasmid
    #53357
    Purpose
    Expresses beta-lactamase for the degradation of beta-lactam antibiotics and tags it with hlyAs (transporter gene) in preparation for secretion from the cell.
    Depositor
    Lingchong You
    Article
    Pai et al Proc Natl Acad Sci U S A. 2012 Nov 27;10
    Inserts
    BlaMs
    hlyAs
    Promoter
    p(lac/ara-1)
    Available Since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    53357-plasmid-map-sequence-id-83079

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  6. pET3C-ARL16(CO)-His6

    Plasmid
    #216485
    Purpose
    Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.
    Depositor
    Richard Kahn
    Insert
    ADP-ribosylation factor-like 16 (ARL16 Human)
    Tags
    includes GSGSGSHHHHHH (His6) tag at C-terminus
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216485-plasmid-map-sequence-id-426900

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  8. p-dCas9-DNMT3a-Hygro

    Plasmid
    #104403
    Purpose
    transient expression of dCas9-DNMT3a fusion protein
    Depositor
    Stephan Beck
    Insert
    DNMT3a (DNMT3A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104403-plasmid-map-sequence-id-204468

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  10. pAAV hSyn1 bPAC cMyc S27A 2A tDimer

    Plasmid
    #85398
    Purpose
    humanized photoactivated adenylyl cyclase lower dark activity, spectrum shifted 15 nm longer wavelenght with neuron-specific promoter, plus red fluorescent protein
    Depositor
    Thomas Oertner
    Inserts
    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    Use
    AAV
    Tags
    cMyc Tag
    Expression
    Mammalian
    Mutation
    S27A
    Promoter
    human Synapsin1 promotor and ribosomal skip seque…
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    85398-plasmid-map-sequence-id-201308

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  11. Lamin A (R482Q)-mRFP

    Plasmid
    #124273
    Purpose
    Lamin A mutant (R482Q), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R482Q) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R482Q)
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124273-plasmid-map-sequence-id-241964

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  12. Hs.KRAS4a G13D

    Plasmid
    #83170
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G13D mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Mutation
    G13D
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83170-plasmid-map-sequence-id-158133

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  13. pKG113

    Plasmid
    #63073
    Purpose
    Retroviral expression of EGFP-TEV-S-tag CENP-T for protein localization and affinity purification (LAP)
    Depositor
    Iain Cheeseman
    Insert
    CENP-T (CENPT Human)
    Use
    Retroviral
    Tags
    EGFP and TEV-S
    Expression
    Mammalian
    Mutation
    E. coli codon optimized. G217R mutation compared …
    Available Since
    Jan. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63073-plasmid-map-sequence-id-134725

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  14. pBabe-Kras G15A

    Plasmid
    #58898
    Purpose
    Expresses human Kras with a G15A mutation
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G15A mutation
    Available Since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. HSD17B11

    Plasmid
    #39159
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B11 (HSD17B11 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. pET28-H12TEV-hEIF5B-1~1220(FL)

    Plasmid
    #208636
    Purpose
    for E. coli expression of human eIF5B (ENST00000289371)
    Depositor
    Quanfu Ma
    Insert
    human eIF5B (ENST00000289371) (EIF5B Human)
    Tags
    6xhis
    Expression
    Bacterial
    Mutation
    Contain SNP rs1376443, protein sequence not chang…
    Promoter
    T7
    Available Since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    208636-plasmid-map-sequence-id-433645

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  18. MAP3K3

    Plasmid
    #38937
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAP3K3 (MAPK3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid
    #69548
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69548-plasmid-map-sequence-id-122974

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  21. Lamin A (R298C)-mRFP

    Plasmid
    #124269
    Purpose
    Lamin A mutant (R298C), C-terminally tagged with Mrfp. Mutation associated with CMT neuropathy.
    Depositor
    Eric Schirmer
    Insert
    LMNA (R298C) (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    LMNA (R298C)
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124269-plasmid-map-sequence-id-241969

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  22. pDONR221_SMAD2_HAe2_Bsd

    Plasmid
    #167166
    Purpose
    Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.
    Depositor
    Alexander Loewer
    Insert
    Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
    Use
    Repair template for gene editing in human cells
    Tags
    blasticidin
    Available Since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167166-plasmid-map-sequence-id-330264

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  23. SDC229-B03

    Plasmid
    #58763
    Purpose
    bacterial expression of PDB 4PYZ
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4PYZ (USP7 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    Aug. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. Syn-ChR-tdTomato

    Plasmid
    #99843
    Purpose
    AAV expression of ChR2(H134R)-tdTomato under the Syn promoter
    Depositor
    Gordon Shepherd
    Insert
    ChR2(H134R)-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    ChR2(H134R), carrying a single point mutation at …
    Promoter
    human synapsin I promoter
    Available Since
    Jan. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    99843-plasmid-map-sequence-id-202528

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  25. tet-pLKO.puro_shHuR 3UTR

    Plasmid
    #110412
    Purpose
    TRCN0000276186 (Target TTGTTAGTGTACAACTCATTT), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 (HuR)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110412-plasmid-map-sequence-id-213309

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  26. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid
    #198217
    Purpose
    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Transposon
    Tags
    3’ part of TurboID, starting with G257 and amino …
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198217-plasmid-map-sequence-id-389406

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  27. AKT3

    Plasmid
    #39038
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AKT3 (AKT3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. huntingtin_V2095-V3138_CbioCflag

    Plasmid
    #194764
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV and P10
    Available Since
    July 27, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194764-plasmid-map-sequence-id-402574

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  29. huntingtin_T97-M2069_NbioCflag

    Plasmid
    #194763
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    Avi-tag (biotin acceptor peptide) and Flag-tag
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV and P10
    Available Since
    July 26, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    194763-plasmid-map-sequence-id-402570

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  30. MS2_SC2_S_Omicron

    Plasmid
    #180229
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)
    Depositor
    Paul Freemont
    Inserts
    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    Expression
    Bacterial
    Mutation
    Omicron (Amino Acid 319-869)
    Promoter
    T7
    Available Since
    Jan. 28, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    180229-plasmid-map-sequence-id-352514

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Showing: 7111 - 7140 of 9621 results