Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 18,127 results for: Mut

Showing: 9481 - 9520 of 18127 results

  1. V5-deltaUBA-short-UBASH3A

    Plasmid
    #195405
    Purpose
    Mammalian expression of human short-form UBASH3A lacking the UBA domain with V5 tag
    Depositor
    Patrick Concannon
    Insert
    UBASH3A (UBASH3A Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    UBASH3A mutant lacking the UBA domain
    Promoter
    Available Since
    March 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    195405-plasmid-map-sequence-id-386309

    Enlarge

  2. pAc5.1B-lambdaN-HA-DmPiwi_O

    Plasmid
    #147072
    Purpose
    Insect Expression of DmPiwi
    Depositor
    Elisa Izaurralde
    Insert
    DmPiwi (piwi Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    first 42 AA missing and two non silent mutations …
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147072-plasmid-map-sequence-id-289519

    Enlarge

  3. pAc5.1B-lambdaN-HA-DmUPF3_1-121-V5His6_N

    Plasmid
    #147005
    Purpose
    Insect Expression of DmUPF3_1-121
    Depositor
    Elisa Izaurralde
    Insert
    DmUPF3_1-121 (Upf3 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    2 silent mutations compared to the sequence given…
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147005-plasmid-map-sequence-id-289452

    Enlarge

  4. pAc5.1B-EGFP-Dm4EHP-A_G

    Plasmid
    #146346
    Purpose
    Insect Expression of Dm4EHP-A
    Depositor
    Elisa Izaurralde
    Insert
    Dm4EHP-A (4EHP Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    4 silent mutations compared to the sequence from …
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146346-plasmid-map-sequence-id-288791

    Enlarge

  5. ipUSEPR-sg-Hs-CD4

    Plasmid
    #188691
    Purpose
    sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgCD4 (CD4 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available Since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188691-plasmid-map-sequence-id-375118

    Enlarge

  6. pAc5.1B-lambdaN-HA-DmAUB_A

    Plasmid
    #145851
    Purpose
    Insect Expression of DmAUB
    Depositor
    Elisa Izaurralde
    Insert
    DmAUB (aub Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    Two silent mutations compared to the sequence giv…
    Promoter
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    145851-plasmid-map-sequence-id-288296

    Enlarge

  7. DsRed-CPI-17-T38A-NLS

    Plasmid
    #100792
    Purpose
    Mammalian expression of Fluorescent CPI-17 neutral mutation nuclear
    Depositor
    Joseph Gordon
    Insert
    protein phosphatase 1 regulatory inhibitor subunit 14A (PPP1R14A Human)
    Use
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    T38A
    Promoter
    CMV
    Available Since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    100792-plasmid-map-sequence-id-193236

    Enlarge

  8. pJK561

    Plasmid
    #72283
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin with Cys35>Ser mutant (AaTrxA-C35S)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes cysteine-35 to serine
    Promoter
    T7
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72283-plasmid-map-sequence-id-131874

    Enlarge

  9. pJK614

    Plasmid
    #72387
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 4 silent mutations in codons 9-11 and 42…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72387-plasmid-map-sequence-id-133927

    Enlarge

  10. pJK621

    Plasmid
    #72388
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurDH6)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72388-plasmid-map-sequence-id-133928

    Enlarge

  11. pJK592

    Plasmid
    #72386
    Purpose
    Produces Escherichia coli glycinamide ribonucleotide synthetase (EcPurD)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylamine-glycine ligase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    contains 3 silent mutations in codons 9-11 to fac…
    Promoter
    T7
    Available Since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72386-plasmid-map-sequence-id-133922

    Enlarge

  12. pJK648

    Plasmid
    #71611
    Purpose
    Produces Treponema denticola 5-aminoimidazole ribonucleotide carboxylase, Phe79>Trp mutant (TdPurE2-F79W)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole carboxylase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes phenylalanine-79 to tryptophan
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71611-plasmid-map-sequence-id-131688

    Enlarge

  14. pET-Impact-T4gp43(exo-)

    Plasmid
    #228202
    Purpose
    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A
    Depositor
    Stephen Benkovic
    Insert
    43
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated aspartate 219 to alanine (D219A), elimina…
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228202-plasmid-map-sequence-id-453943

    Enlarge

  15. 8522-E06

    Plasmid
    #234291
    Purpose
    Gateway ORF Entry clone of human Tgfbr1 with stop codon (for native or N-terminal fusions); T204D mutation
    Depositor
    Dominic Esposito
    Insert
    Tgfbr1 T204D (Tgfbr1 Mouse)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    T204D
    Promoter
    Available Since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    234291-plasmid-map-sequence-id-468906

    Enlarge

  16. pSL1423

    Plasmid
    #194904
    Purpose
    pSLI-TGD/TurboID, C-terminal tag of recodonized TurboID for Plasmodium falciparum
    Depositor
    Scott Lindner
    Insert
    TurboID
    Use
    Plasmodium falciparum
    Tags
    GFPmut2
    Expression
    Mutation
    Promoter
    None. This allows a C-terminal fusion to a protei…
    Available Since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    194904-plasmid-map-sequence-id-382850

    Enlarge

  17. pT7-V5-SBP-C1-HsUpf1-siRNAres_P

    Plasmid
    #147196
    Purpose
    Mammalian Expression of HsUpf1-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsUpf1-siRNAres (UPF1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5 non silent mutations A69S, E167G, K282R, A442G…
    Promoter
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147196-plasmid-map-sequence-id-429667

    Enlarge

  18. PGAM5

    Plasmid
    #38976
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PGAM5 (PGAM5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pcDNA3.1-hRIPK1-Flag-delC

    Plasmid
    #112493
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of C terminus (AA290-671 deleted)
    Promoter
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112493-plasmid-map-sequence-id-214530

    Enlarge

  20. Phage-PGK-GFP-T(WT)

    Plasmid
    #181736
    Purpose
    for lentiviral expression of brachyury ORF in mammalian cells
    Depositor
    Charles Lin
    Insert
    T (brachyury)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    a silent mutation in the PAM site of an sgRNAt ta…
    Promoter
    Available Since
    Aug. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    181736-plasmid-map-sequence-id-357223

    Enlarge

  21. pAAV-CaMKIIa-HA-rM3D(Gs)-IRES-mCitrine

    Plasmid
    #50468
    Purpose
    Gs-coupled rM3D-IRES-mCitrine under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Insert
    rM3D-IRES-mCitrine
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50468-plasmid-map-sequence-id-75484

    Enlarge

  23. pmSod1-G93A-N’His

    Plasmid
    #117705
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    G93A
    Promoter
    T7
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117705-plasmid-map-sequence-id-227526

    Enlarge

  24. pcDNA3.1-hRIPK1-Flag-delN

    Plasmid
    #112492
    Purpose
    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of N terminus (AA1-274 deleted)
    Promoter
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112492-plasmid-map-sequence-id-214533

    Enlarge

  26. pcDNA3.1-hRIPK1-Flag-delDD

    Plasmid
    #112494
    Purpose
    Mammalian expression of the indicated Flag- hRIPK1 mutant. Please note Flag tag is N-terminal.
    Depositor
    Junying Yuan
    Insert
    RIPK1 (RIPK1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    deletion of DD domain (AA584-671 deleted)
    Promoter
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112494-plasmid-map-sequence-id-214528

    Enlarge

  27. XClone_Cas9_KRAB_T2A_BFP

    Plasmid
    #141208
    Purpose
    Tunable and temporal expression control of dCas9
    Depositor
    Rory Johnson
    Insert
    dCas9
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    141208-plasmid-map-sequence-id-308770

    Enlarge

  28. pMCP-tag (m) Vector

    Plasmid
    #101127
    Purpose
    Mammalian expression plasmid that allows for the expression of a gene of interest fused to the N-terminus or C-terminus of MCP-tag.
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Use
    Tags
    MCP-tag (Mutant ACP- tag)
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101127-plasmid-map-sequence-id-194153

    Enlarge

  29. pMCP-GPI Control Plasmid

    Plasmid
    #101132
    Purpose
    Expression of MCP-Tagged GPI anchor in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Insert
    GPI
    Use
    Tags
    MCP-tag (Mutant ACP- tag)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101132-plasmid-map-sequence-id-194085

    Enlarge

  33. pET-24a-STE2

    Plasmid
    #133430
    Purpose
    Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Both native Cys (C59S/C252A) have been mutated. S…
    Promoter
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133430-plasmid-map-sequence-id-267723

    Enlarge

  36. pRK GFP Paxillin Y31A

    Plasmid
    #50530
    Depositor
    Kenneth Yamada
    Insert
    Paxillin (PXN Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Y31A
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. GST-E6AP C833A

    Plasmid
    #13465
    Depositor
    Michael Greenberg
    Insert
    E6-AP (UBE3A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    C833A; active site Cys mutated to encode Ala
    Promoter
    Available Since
    March 6, 2007
    Availability
    Academic Institutions and Nonprofits only

  38. Phage-PGK-FLAG-HA-T(WT)

    Plasmid
    #181737
    Purpose
    for lentiviral expression of brachyury ORF in mammalian cells
    Depositor
    Charles Lin
    Insert
    T (brachyury)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    a silent mutation in the PAM site of an sgRNAt ta…
    Promoter
    Available Since
    Aug. 3, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    181737-plasmid-map-sequence-id-357225

    Enlarge

  39. pGEX4T1-WWP1F792D

    Plasmid
    #179328
    Purpose
    E. coli optimized expression of GST-fusion of human WWP1 F792D
    Depositor
    Arthur Haas
    Insert
    WWP1 F792D (WWP1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    F792D mutation; Codon Optimized for expression in…
    Promoter
    Available Since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179328-plasmid-map-sequence-id-358707

    Enlarge

  40. ∆DUSP7

    Plasmid
    #27978
    Depositor
    Igor Astsaturov
    Insert
    DUSP7 (DUSP7 Human)
    Use
    Lentiviral
    Tags
    HA and MYC
    Expression
    Mammalian
    Mutation
    C280S: mutation in the phosphatase domain
    Promoter
    Available Since
    Sept. 13, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 9481 - 9520 of 18127 results