We narrowed to 10,664 results for: t7
-
-
-
-
pCMV-eVRQR-P2A-EGFP (KAC1028)
Plasmid#242663PurposeCMV promoter expression plasmid for human codon optimized SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFPDepositorInsertpCMV-SpCas9-eVRQR(S55R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
UseCRISPRTagsBPNLSExpressionMammalianMutationeVRQR mutations in SpCas9(S55R/D1135V/G1218R/R133…PromoterCMV and T7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpCas9-VQR-P2A-EGFP (LRF199)
Plasmid#242664PurposeCMV promoter expression plasmid for human codon optimized SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFPDepositorInsertpCMV-SpCas9-VQR-BPNLS(SV40)-3xFLAG-P2A-EGFP
UseCRISPRTagsBPNLSExpressionMammalianMutationVQR mutations in SpCas9(D1135V/R1335Q/T1337R)PromoterCMV and T7Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-Tn5
Plasmid#60240PurposeIPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.DepositorInserttnpA from pBam1
TagsMxe intein - Chitin-binding domainExpressionBacterialMutationE54K, L372PPromoterT7 promoterAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET30b-5M SrtA with TEV site
Plasmid#86962PurposeExpresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tagDepositorInsertS.aureus SrtA 5M
Tags6x Histidine tag and TEV siteExpressionBacterialMutationP94R, D160N, D165A, K190E, K196TPromoterT7Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1-Strep-II
Plasmid#224209PurposeMembrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.DepositorInsertMSP1D1-Strep-II
TagsStrep-II TagExpressionBacterialMutationSubstitution of 7xHis tag found in pMSP1D1 for a …PromoterT7Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYC-GroEL/ES-TF
Plasmid#83923PurposeCo-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.DepositorInsertsGro EL/ES
Trigger Factor
ExpressionBacterialPromoterT7Available SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
mRNA-CleanCap-mNeonGreen
Plasmid#188248PurposeTemplate plasmid to transcribe mRNA encoding mNeonGreenDepositorInsertmNeonGreen
ExpressionMammalianPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCm C-term MBP NikJC199U
Plasmid#174365PurposeExpression plasmid with a TEV removable C-term 8xHis tagged MBP tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.DepositorInsertnikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
Tags8xHis, MBP, and TEVExpressionBacterialMutationC199UPromoterT7Available SinceDec. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET24a isPETase
Plasmid#225759PurposeExpresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tagDepositorInsertisPETase
Tags6xHis TagExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
bZiPro
Plasmid#86846Purposeexpress NS2B-NS3 protease in E.coliDepositorInsertsNS3 1-177
NS2B45-96
Tagsa hexahistidine tag and a thrombin cleavage siteExpressionBacterialMutationNS2B cofactor region (residues 45–96) and NS3 pro…PromoterT7Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+30)
Plasmid#199164Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+30)
TagsMGHHHHHHGGAMutationE16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
circRNA-synIRES-R25-NanoLuc
Plasmid#188116PurposeTemplate plasmid to transcribe circular RNA encoding NanoLucDepositorInsertNanoLuc
UseLuciferaseExpressionMammalianPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
VR122 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198467PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
Tags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
circRNA-synIRES-R25-mNeonGreen
Plasmid#188115PurposeTemplate plasmid to transcribe circular RNA encoding mNeonGreenDepositorInsertmNeonGreen
ExpressionMammalianPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-MBP-TEV
Plasmid#69929Purposebacterial expression of MBP-TEV n-terminal fusionsDepositorHas ServiceCloning Grade DNATypeEmpty backboneTagsMBP and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available SinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMJ915
Plasmid#69090PurposeExpress Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLSDepositorInsertcas9
TagsMBP, hexa His, and two SV40 NLSExpressionBacterialPromoterT7Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
CRE recombinase
Plasmid#62730PurposeExpresses Cre recombinase protein in bacterial cellsDepositorInsertCre recombinase
UseCre/LoxTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only
