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Showing: 11901 - 11950 of 23156 results
  1. pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut_R

    Plasmid
    #147399
    Purpose
    Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut
    Depositor
    Insert
    CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut (TNRC6C Nematode, Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Deletion of 2 AA (SD) at position 378-379 compare…
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pGADT7-γ1

    Plasmid
    #197078
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Insert
    AP-1 γ1 (Ap1g1 Mouse)
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. pGADT7-ε

    Plasmid
    #197261
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Insert
    AP-4 ε (AP4E1 Human)
    Use
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    silent substitutions in codons 905, 1129 and 1137
    Promoter
    ADH1
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pSN-A112C-1A32

    Plasmid
    #191241
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Feb. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pSN-A112C-1A31

    Plasmid
    #191242
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. KTK_219

    Plasmid
    #180534
    Purpose
    Entry vector containing K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon)
    Depositor
    Insert
    K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. KTK_223

    Plasmid
    #180538
    Purpose
    Entry vector containing K.rhaeticus native promoter pHxu (200 bases upstream from gene start codon)
    Depositor
    Insert
    K.rhaeticus native promoter pHxu (200 bases upstream from gene start codon)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. KTK_226

    Plasmid
    #180552
    Purpose
    Entry vector containing K.rhaeticus native promoter pRutB (200 bases upstream from gene start codon)
    Depositor
    Insert
    K.rhaeticus native promoter pRutB (200 bases upstream from gene start codon)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A_R

    Plasmid
    #147394
    Purpose
    Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A
    Depositor
    Insert
    CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A (TNRC6C Nematode, Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Deletion of 2 AA (SD) at position 378-379 compare…
    Promoter
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. KTK_218

    Plasmid
    #180533
    Purpose
    Entry vector containing K.rhaeticus native promoter pTtaC (200 bases upstream from gene start codon)
    Depositor
    Insert
    K.rhaeticus native promoter pTtaC (200 bases upstream from gene start codon)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  12. KTK_225

    Plasmid
    #180539
    Purpose
    Entry vector containing K.rhaeticus native promoter pBlh (200 bases upstream from gene start codon)
    Depositor
    Insert
    K.rhaeticus native promoter pBlh (200 bases upstream from gene start codon)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  13. KTK_221

    Plasmid
    #180536
    Purpose
    Entry vector containing K.rhaeticus native promoter pKr0953 (200 bases upstream from gene start codon)
    Depositor
    Insert
    K.rhaeticus native promoter pKr0953 (200 bases upstream from gene start codon)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. KTK_220

    Plasmid
    #180535
    Purpose
    Entry vector containing K.rhaeticus native promoter pAacsAB4 (200 bases upstream from gene start codon)
    Depositor
    Insert
    K.rhaeticus native promoter pAacsAB4 (200 bases upstream from gene start codon)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  15. KTK_222

    Plasmid
    #180537
    Purpose
    Entry vector containing K.rhaeticus native promoter pCyd (200 bases upstream from gene start codon)
    Depositor
    Insert
    K.rhaeticus native promoter pCyd (200 bases upstream from gene start codon)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pNOC_dCas9_sgRNA Td2

    Plasmid
    #176258
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  17. pNOC_dCas9_sgRNA Td1

    Plasmid
    #176257
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 1
    Depositor
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. pNOC_dfnCas12a_crRNA Td2

    Plasmid
    #176255
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Expression
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Promoter
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 11901 - 11950 of 23156 results