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We narrowed to 18,247 results for: multi

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Showing: 4761 - 4780 of 18247 results

  2. cfSYFP2-N

    Plasmid
    #110631
    Purpose
    Cys-free yellow fluorescent protein for extracellular imaging. SYFP2 variant (C48S/C70M). Molecular brightness is almost comparable to SYFP2.
    Depositor
    Ikuo Wada
    Insert
    cfSYFP2-N
    Tags
    Multi-cloning sites
    Expression
    Mammalian
    Mutation
    Cys48Ser and Cys70/Met
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    110631-plasmid-map-sequence-id-291941

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  3. pPV476

    Plasmid
    #42929
    Depositor
    James Lok
    Insert
    attR2::egfp (870 bp)::taa::Ss-era-1 terminator (598 bp)::attL3
    Use
    Multi-site gateway donor plasmid
    Available since
    March 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    42929-plasmid-map-sequence-id-58192

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  4. pPV475

    Plasmid
    #42928
    Depositor
    James Lok
    Insert
    attR2::Ss-era-1 terminator (598 bp)::attL3
    Use
    Multi-site gateway donor plasmid
    Available since
    March 4, 2013
    Availability
    Academic Institutions and Nonprofits only
    42928-plasmid-map-sequence-id-58191

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  6. pHelper_TS_V2_ampR

    Plasmid
    #214467
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.
    Depositor
    Scott Saunders
    Publication
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Promoter
    XylS / AraC
    Available since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    214467-plasmid-map-sequence-id-429338

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  14. pSPObooster

    Plasmid
    #216160
    Purpose
    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains
    Depositor
    Grant Brown
    Publication
    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978
    Inserts
    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    Expression
    Yeast
    Mutation
    MKT1 gene containing an aspartic acid to glycine …
    Promoter
    endogenous promoter
    Available since
    May 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216160-plasmid-map-sequence-id-433867

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  15. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid
    #154840
    Purpose
    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.
    Depositor
    Patrick Phillips
    Publication
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    Use
    CRISPR and Cre/Lox
    Expression
    Worm
    Mutation
    HYGR∆ encodes aa 1-226
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154840-plasmid-map-sequence-id-307910

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  16. SpCas9-HF1

    Plasmid
    #138556
    Purpose
    Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138556-plasmid-map-sequence-id-274763

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  17. tetO-Bcl2-IRES-tdtomato(TET005)

    Plasmid
    #117857
    Purpose
    This plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.
    Depositor
    C. Ron Yu
    Publication
    Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bcl2 (Bcl2 Mouse)
    Use
    Tta/teto
    Expression
    Mammalian
    Promoter
    tetO
    Available since
    May 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117857-plasmid-map-sequence-id-245325

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  18. pcDNA3-RAB-AKARev

    Plasmid
    #118478
    Purpose
    ddRFP-based single-color biosensor for monitoring PKA (Protein Kinase A) activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB-AKARev
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    118478-plasmid-map-sequence-id-243122

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  19. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104910-plasmid-map-sequence-id-206061

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Showing: 4761 - 4780 of 18247 results