We narrowed to 17,934 results for: multi
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Plasmid#222139PurposeNear PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertTadA-nSpRYCas9
UseCRISPR; Zebrafish expressionTagsbipartite NLSPromoterT3Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCsy_complex
Plasmid#89232PurposePlasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage siteDepositorInsertsCsy1
Csy2
Csy3
Csy4
Tags6xHis-TEV proteaseAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Mis12-targted Aurora B FRET sensor
Plasmid#45231DepositorInsertMis12 targeted Aurora B FRET sensor
ExpressionMammalianAvailable sinceMay 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
Tol2-CAG::Nucbow
Plasmid#158992PurposeMulticolor nuclear-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing H2B-tethered mEYFP, mCherry or mCerulean upon Cre recombination)DepositorInsertNucbow
TagsAll FPs are fused to H2BExpressionMammalianPromoterCAGAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSP3545-dCas9Str
Plasmid#153516PurposeExpresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backboneDepositorInsertdCas9Str
ExpressionBacterialPromoternisAAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFD4d-U6-1:white1-U6-3:white1
Plasmid#84005PurposepCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoterDepositorInsertswhite sgRNA-1
white sgRNA-1
UseCRISPRPromoterU6:1, U6:3, and dU6-1; dU6-3Available sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEM047 [T7-assisted CROPseq sgRNA expression vector]
Plasmid#224899PurposeLentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNADepositorInsertseGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGLTR-FP-GFP
Plasmid#58242PurposeGATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP markerDepositorInsertattR1-Cm(R)-CcdB-attR2
UseLentiviral and RNAi; Gateway destionation vectorExpressionMammalianPromoternoAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
TRE-VEGFA EF1a-rtTA3
Plasmid#195881PurposeLentiviral construct of doxycycline inducible VEGFADepositorAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBPhsFlp2::PEST
Plasmid#63171PurposeDrosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock PromoterDepositorInserthsFlp2::PEST
ExpressionInsectAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLX-B2 (GB3374)
Plasmid#160636PurposepLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)DepositorTypeEmpty backboneUseSynthetic BiologyAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM126
Plasmid#105134PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)
Plasmid#92401PurposeConstitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and membrane tagPromoterbactin2Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Frt-Atg-Hygro-CMV-green (mWasabi)-PA-F3
Plasmid#67276PurposeRMCE donor plasmid for FlpO. the marker gene is green fluorescent (mWasabi) with PADepositorInsertsGFP (mWasabi)
hygro
TagsF3 and FrtExpressionMammalianPromoterCMVAvailable sinceJuly 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)
Plasmid#154840PurposeInsertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.DepositorInserthomology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
UseCRISPR and Cre/LoxExpressionWormMutationHYGR∆ encodes aa 1-226Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVITRO1-102.1F10-IgA2/λ
Plasmid#50371PurposeExpresses grass pollen allergen Phl p 7 specific human IgA2/λ antibody isotype (102.1F10-IgA2/λ)DepositorInsertsgrass pollen allergen Phl p 7 specific human Alpha 2 heavy chain expression cassette
grass pollen allergen Phl p 7 specific human lambda light chain expression cassette
ExpressionMammalianPromoterMouse Elongation Factor 1 Alpha and Rat Elongatio…Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTol2CG2-QUAS5x:GFPNLS-SV40pA
Plasmid#155122PurposeTol2 vector containing QUAS5x reporter element upstream of GFP-NLS. Includes cmlc2:mRFP-SV40pA transgenesis markerDepositorInsertQUAS5x:GFPNLS
UseZebrafish expressionPromoterQUAS5x-E1bAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)
Plasmid#48294DepositorTypeEmpty backboneExpressionInsectAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pemiRFP703-clathrin
Plasmid#136563PurposeClathrin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertemiRFP703-clathrin
TagsemiRFP703ExpressionMammalianPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-PKI-GFP
Plasmid#118482PurposeGFP-tagged Protein Kinase A inhibitor peptide.DepositorInsertPKI-GFP
TagsmEGFPExpressionMammalianPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by