We narrowed to 15,071 results for: nol

1. pAs-Cpf1-pGL3-U6-sgRNA

   Plasmid

   #107683

   Purpose

   Expresses AsCpf1 crRNA in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR2-R2-PBR-L4

   Plasmid

   #141017

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR2-L3-PBL-R1

   Plasmid

   #141016

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJY3948

   Plasmid

   #113338

   Purpose

   6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

   Depositor

   Suzanne Sandmeyer

   Article

   Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

   Insert

   2-pyrone synthase, codon optimized for Y. lipolytica
   

   Use

   Tags

   6XHis

   Expression

   Yeast

   Mutation

   Please see depositor comments

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB2227

   Plasmid

   #67536

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB2190

   Plasmid

   #67535

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCR1055

   Plasmid

   #71823

   Purpose

   Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR-P2rP4

   Plasmid

   #141015

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMK390(Stag-3FLAG-BSD)

   Plasmid

   #121191

   Purpose

   C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Stag-3FLAG-BSD
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCellFree_G10

    Plasmid

    #67144

    Purpose

    Gateway destination vector for cell-free expression with C-term sfGFP 8xHis tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    HIS and sfGFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB4786

    Plasmid

    #106142

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TAL2369

    Plasmid

    #36780

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    NCOR1-TALEN_Right (Ncor1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

13. TALEN1259

    Plasmid

    #32284

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting gria3a (gria3a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBT138b-proA

    Plasmid

    #138514

    Purpose

    P3g

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCellFree_G03 SP2

    Plasmid

    #67109

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    SP2 (SP2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pBbE1k-2

    Plasmid

    #59945

    Purpose

    dihydropterine reductase from human, pterin-4a-carbinolamine dehydratase from human

    Depositor

    Taek Soon Lee

    Article

    Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

    Inserts

    dihydropterine reductase
    pterin-4a-carbinolamine dehydratase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTrc

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFREE2-xCas9-sgRNA

    Plasmid

    #179579

    Purpose

    Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    xCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV anti-FITC-LaG16-Y192A-T2A-BFP

    Plasmid

    #194933

    Purpose

    LV construct encoding Y192A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y192A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pASKt15C+SrtA

    Plasmid

    #64983

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. TALEN1258

    Plasmid

    #32281

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting gria3a (gria3a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and FOKI WT

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge