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Showing: 1151 - 1200 of 4900 results

  9. bbCas9pluspAAA

    Plasmid
    #82581
    Purpose
    The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA
    Depositor
    Ivo Huijbers
    Article
    Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:
    Insert
    Cas9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    82581-plasmid-map-sequence-id-228465

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  39. pAAV-SCP1-dSa-VPR mini.

    Plasmid
    #99685
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    99685-plasmid-map-sequence-id-194598

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  42. Level 1 P4 TaU6 guide acceptor

    Plasmid
    #165600
    Purpose
    GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Insert
    TaU6 promoter::LacZ::sgRNA scaffold
    Use
    CRISPR and Synthetic Biology; Sgrna acceptor with…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165600-plasmid-map-sequence-id-331273

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  43. Level 1 P3 TaU6 guide acceptor

    Plasmid
    #165599
    Purpose
    GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Article
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Insert
    TaU6 promoter::LacZ::sgRNA scaffold
    Use
    CRISPR and Synthetic Biology; Sgrna acceptor with…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165599-plasmid-map-sequence-id-331270

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  45. KG#62

    Plasmid
    #110874
    Purpose
    Expresses the C. elegans acy-1 cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1
    Inserts
    rab-3 promoter
    acy-1 cDNA
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110874-plasmid-map-sequence-id-212583

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  46. pAAV-EFS-dSa-VPR mini.

    Plasmid
    #99686
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from EFS promoter
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    99686-plasmid-map-sequence-id-194608

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Showing: 1151 - 1200 of 4900 results