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Showing: 461 - 480 of 2310 results

  1. pDM026

    Plasmid
    #216808
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216808-plasmid-map-sequence-id-429385

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  3. 3xFLAG-dCas9/pMXs-puro

    Plasmid
    #51240
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    51240-plasmid-map-sequence-id-410075

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  5. pMH-Cas9-gate

    Plasmid
    #113742
    Purpose
    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    Cas9
    Use
    CRISPR; Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Z. Mays ubiquitin promoter, 35S promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113742-plasmid-map-sequence-id-238953

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  7. 3xFLAG-dCas9/pMXs-neo

    Plasmid
    #51260
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Feb. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    51260-plasmid-map-sequence-id-77871

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  10. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    51258-plasmid-map-sequence-id-77869

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  11. pBS SK mCherryROSAbsr

    Plasmid
    #54321
    Purpose
    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.
    Depositor
    Silvestro Conticello
    Article
    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.
    Insert
    mCherry and BlasticidinS-resistance
    Use
    CRISPR, Mouse Targeting, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    54321-plasmid-map-sequence-id-84455

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  13. pZeo-Cas9-gate

    Plasmid
    #113744
    Purpose
    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving Zeocin for selection in plants.
    Depositor
    Magdalena Bezanilla
    Article
    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d
    Insert
    Cas9
    Use
    CRISPR; Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Z. Mays ubiquitin promoter, 35S promoter
    Available since
    Sept. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    113744-plasmid-map-sequence-id-219786

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  16. pTX198

    Plasmid
    #89262
    Purpose
    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01
    Depositor
    Daniel Voytas
    Article
    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:
    Insert
    OsPDS-gRNA01
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    Maize Ubiquitin1 promoter
    Available since
    May 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    89262-plasmid-map-sequence-id-177836

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  19. pMG

    Plasmid
    #138264
    Purpose
    Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Inserts
    eGFP flanked by iCas9 sites
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tef1
    Available since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138264-plasmid-map-sequence-id-273626

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  20. pXGFPC-5 Pxyl-dcas9 (Spy)

    Plasmid
    #133316
    Purpose
    expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus pyogenes)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    xylose
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    133316-plasmid-map-sequence-id-266825

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Showing: 461 - 480 of 2310 results