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pcDNA3 Ash2L full

Plasmid

#15548

Depositor

Kai Ge

Publication

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Ash2L (ASH2L Human)

Expression

Mammalian

Mutation

R622H (Please see depositor comments below)

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-HA-ADH

Plasmid

#11610

Depositor

Steven Johnson

Insert

aldehyde dehydrogenase (ALDH1A1 Human)

Tags

HA

Expression

Mammalian

Mutation

K412I

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pC.HA Vav2

Plasmid

#14554

Depositor

Joan Brugge

Publication

Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

Insert

Vav2 (VAV2 Human)

Tags

HA

Expression

Mammalian

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pBS mShh (CT#258)

Plasmid

#13999

Depositor

Cliff Tabin

Insert

Shh (Shh Mouse)

Expression

Bacterial

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA-hISG15

Plasmid

#12447

Depositor

Dong-Er Zhang

Publication

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

ISG15 fragment (ISG15 Human)

Expression

Mammalian

Mutation

ISG15 up to LRLRGG

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRK5-myc-Cdc42-T17N

Plasmid

#12973

Depositor

Gary Bokoch

Insert

Cdc42 DN (CDC42 Human)

Tags

Myc

Expression

Mammalian

Mutation

T17N Dominant negative

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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HA.PKD.K/W

Plasmid

#10809

Depositor

Alex Toker

Publication

Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

Insert

PKD (PRKD1 Human)

Tags

HA and Myc (inserted between amino acids 33&3…

Expression

Mammalian

Mutation

Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

Promoter

CMV

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pcDNA3 HA Arf1 DN T31N

Plasmid

#10833

Depositor

Thomas Roberts

Publication

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 T31N (ARF1 Human)

Tags

HA

Expression

Mammalian

Mutation

T31N. Defective in GTP binding. Dominant negati…

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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PGC-1 alpha promoter luciferase delta MEF

Plasmid

#8889

Depositor

Bruce Spiegelman

Publication

Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

Insert

PGC-1 alpha promoter dMEF (Ppargc1a Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Site-directed mutagenesis to remove the MEF2 bind…

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBABE puro PPAR delta

Plasmid

#8891

Depositor

Bruce Spiegelman

Publication

Brun et al Genes Dev. 1996 Apr 15. 10(8):974-84.

Insert

PPAR delta (Ppard Mouse)

Use

Retroviral

Expression

Mammalian

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV-evoCAST-Cas6

Plasmid

#234725

Purpose

Engineered human-codon optimized PseCas6 in mammalian cells (for evoCAST)

Depositor

David Liu

Publication

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PseCas6

Expression

Mammalian

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDAC565

Plasmid

#195242

Purpose

Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)

Depositor

Jennifer Doudna

Publication

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA

Expression

Mammalian

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lentiCRISPRi(v2)-Blast

Plasmid

#170068

Purpose

Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

Depositor

Neville Sanjana

Publication

Morris et al Science. 2023 May 19;380(6646):eadh7699.

Insert

KRAB-dCas9-MeCP2

Use

CRISPR and Lentiviral

Tags

2A and 2xHA tag

Expression

Mammalian

Promoter

EFS-NS

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCW57.1 CMYC

Plasmid

#164145

Purpose

Doxycycline inducible expression of c-MYC

Depositor

Richard Possemato

Insert

MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Doxycycline inducible promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PX458-mcherry

Plasmid

#161974

Purpose

Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

Depositor

Joanna Wysocka

Publication

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

Cas9-t2a-mcherry

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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mCh-Mfn2

Plasmid

#141156

Purpose

Mammalian expression and labeling mitofusin 2

Depositor

Gia Voeltz

Publication

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 2 (MFN2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pXG237

Plasmid

#141281

Purpose

ATF4 translational reporter

Depositor

Martin Kampmann

Publication

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

uORF1/2 of ATF4 followed by mApple

Use

Lentiviral

Tags

mApple

Promoter

CMV

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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8xNFAT-ZsG-hCD8

Plasmid

#153417

Purpose

NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

Depositor

Maki Nakayama

Publication

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )

Use

Retroviral

Expression

Mammalian

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRN227

Plasmid

#127222

Purpose

BeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Publication

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Cas9, WUS2, AtSTM, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

Plasmid

#126580

Purpose

A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Publication

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Tags

HA

Expression

Mammalian

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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