We narrowed to 79,094 results for: Mycs;

1. pET28a(+)-HisTim12_CtoA

   Plasmid

   #170281

   Purpose

   Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

   Insert

   yeast Tim12 (TIM12 Budding Yeast)
   

   Use

   Tags

   TEV-cleavable N-terminal His tag (on backbone)

   Expression

   Bacterial

   Mutation

   mutated two non-conserved Cys to Ala

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCSN071

   Plasmid

   #166729

   Purpose

   Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

   Depositor

   Rene Verwaal

   Article

   Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

   Insert

   dCas12a-Mxi1-NLS
   

   Use

   CRISPR

   Tags

   Mxi1 and SV40 NLS

   Expression

   Yeast

   Mutation

   E925A

   Promoter

   S. cerevisiae TEF1

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSN072

   Plasmid

   #166730

   Purpose

   Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae

   Depositor

   Rene Verwaal

   Article

   Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

   Insert

   dCas12a-NLS-Mxi1-NLS
   

   Use

   CRISPR

   Tags

   Mxi1 and SV40 NLS

   Expression

   Yeast

   Mutation

   E925A

   Promoter

   S. cerevisiae TEF1

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)

   Plasmid

   #164916

   Purpose

   Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR

   Depositor

   Nicholas Ingolia

   Article

   Muller et al Science. 2020 Dec 11;370(6522). pii: 370

   Insert

   GCN4(UTR)-ZEM(TF)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PGK1 (yeast)

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPS1-Venus

   Plasmid

   #133118

   Purpose

   Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pYPS1 driving a Venus.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   pYPS1-YFP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMK2-Venus

   Plasmid

   #133119

   Purpose

   Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pCMK2 driving a Venus.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   pCMK2-YFP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGYP7-Venus

   Plasmid

   #133120

   Purpose

   Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pGYP7 driving a Venus.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   pGYP7-YFP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJL1-neoR

   Plasmid

   #117048

   Purpose

   In vitro expression of neoR (kanamycin resistance gene) from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

   Insert

   neoR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pILGFP88

   Plasmid

   #83544

   Purpose

   Used to evaluate the expression output of RPL15A promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   RPL15A promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   RPL15A

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pILGFP77

    Plasmid

    #83538

    Purpose

    Used to evaluate the expression output of RPL8B promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    RPL8B promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    RPL8B

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pILGFPH3

    Plasmid

    #83553

    Purpose

    Used to evaluate the expression output of HXT7 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    HXT7 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    HXT7

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pILGFPH0

    Plasmid

    #83552

    Purpose

    Used to evaluate the expression output of TPS1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TPS1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TPS1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pILGFP90

    Plasmid

    #83547

    Purpose

    Used to evaluate the expression output of TEF2 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF2 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF2

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pILGFP82

    Plasmid

    #83541

    Purpose

    Used to evaluate the expression output of YEF3 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    YEF3 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    YEF3

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pILGFP80

    Plasmid

    #83540

    Purpose

    Used to evaluate the expression output of RPL3promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    RPL3 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    RPL3

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pILGFP78

    Plasmid

    #83539

    Purpose

    Used to evaluate the expression output of SSB1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    SSB1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SSB1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGMCK-0X750-luxAB-DAS+4

    Plasmid

    #49517

    Purpose

    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; integrates into the mycobacteriophage att-L5 site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    P750-luxAB-DAS+4
    

    Use

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Mutation

    Promoter

    P750

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dom34 E23K/F218A

    Plasmid

    #19106

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    E23K/F218A

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pScSR beta deltaN Flag

    Plasmid

    #13729

    Depositor

    Thomas Schwartz

    Article

    Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

    Insert

    Signal recognition particle receptor β subunit (SRP102 Budding Yeast)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Mutation

    expresses residues 31-244 of S. cerevisiae SRβ wi…

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-LifeAct-FRB-DMVF-181-N

    Plasmid

    #87610

    Purpose

    FRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    LifeAct-FRB-DMVF181-N
    

    Use

    Tags

    17-aa sequence from Abp140 (aka "Lifeact&quo…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDSM-cd-Pfba1p-ACO-Tgpm1

    Plasmid

    #127731

    Purpose

    Yeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    aconitase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pfba1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDSM-cd-Pykt6-ACO-Tprm9

    Plasmid

    #127732

    Purpose

    Yeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    aconitase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pykt6

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDSM-cd-Ptef1-ACO-Tyol036w

    Plasmid

    #127733

    Purpose

    Yeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    aconitase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Ptef1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDSM-cd-Psptdh3-ACO-Tagtef1

    Plasmid

    #127734

    Purpose

    Yeast pathway position 4. ACO transcription unit with the S. paradoxus TDH3 promoter and A. gossypii TEF1 terminator.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Insert

    aconitase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Psptdh3

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGIP

    Plasmid

    #26777

    Purpose

    Lentiviral vector for stable integration of GFP expression cassette with puromycin selection.

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    EF1a-GFP-IRES-Puro (gfp )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-FLAG-MTOR-S2215Y

    Plasmid

    #69013

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S2215Y

    Promoter

    CMV

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AIO-Puro

    Plasmid

    #74630

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Puromycin resistant marker

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAVS1-P-CAG-GFP

    Plasmid

    #80491

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    CAG-GFP
    

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HDAC6 Flag

    Plasmid

    #13823

    Purpose

    Mammalian expression of human histone deacetylase 6 with flag tag

    Depositor

    Eric Verdin

    Article

    Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

    Insert

    HDAC6 (HDAC6 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLKO5.sgRNA.EFS.PAC

    Plasmid

    #57825

    Purpose

    Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge