We narrowed to 7,491 results for: RAP

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pcDNA3 S6K2 E388 D3E

Plasmid

#17731

Depositor

John Blenis

Article

Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

Insert

S6K2 (RPS6KB2 Human)

Tags

HA

Expression

Mammalian

Mutation

T388E, D3E(S410D, S417D, S423D): rapamycin-resist…

Promoter

CMV

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

Plasmid

#172066

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-CD9-10

Plasmid

#57356

Purpose

Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CD9 (CD9 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
FRB_nSbMVp

Plasmid

#118969

Purpose

Expresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nSbMVp in fusion with FRB

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro

Plasmid

#170467

Purpose

The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

Depositor

Raphael Gaudin

Article

Bakhache et al bioRxiv 2021

Insert

Nucleocapsid

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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GST-MAGE-2-429

Plasmid

#89129

Purpose

bacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

MAGE-A11 (MAGEA11 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-eBE-S3

Plasmid

#101093

Purpose

Expresses eBE-S3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

eBE-S3

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGEL033

Plasmid

#137902

Purpose

STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP1363

Plasmid

#119279

Purpose

for stabilizing ICE in the presence of rapI expression

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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FRB_nPPVp

Plasmid

#118966

Purpose

Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain

Depositor

Roman Jerala

Article

Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

Insert

split nPPVp in fusion with FRB

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE N-mRuby3 (from SARS-CoV-2) IRES puro

Plasmid

#170466

Purpose

The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mRuby3 fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

Depositor

Raphael Gaudin

Article

Bakhache et al bioRxiv 2021

Insert

Nucleocapsid

Use

Lentiviral

Tags

mRuby3

Expression

Mammalian

Promoter

EF1

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJT42_GalL_BE-PAPAPA

Plasmid

#145035

Purpose

Expresses BE-PAPAPA in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-PAPAP

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJT43_GalL_BE-P(AP)3

Plasmid

#145036

Purpose

Expresses BE-P(AP)3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-(AP)3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pB80-FRB-GFP-ppKin14VIb(861-1321)

Plasmid

#174638

Purpose

Rapalog-inducible coupling to dimeric moss kinesin-14 for retrograde transport via FRB

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

FRB-GFP-ppKin14VIb(861-1321)

Tags

FRB-GFP

Expression

Mammalian

Mutation

EGFP: Met1Del; ppKin14VIb(aa861-1321)

Promoter

Chicken beta-actin

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-2Aneo

Plasmid

#80032

Purpose

Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

Depositor

Hiroyuki Konishi

Article

Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

Type

Empty backbone

Use

AAV and Cre/Lox; A platform vector to construct t…

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pEF HA-TSAdwt

Plasmid

#84517

Purpose

Mammalian expression of TSAd

Depositor

Anne Spurkland

Article

Granum et al BMC Immunol. 2006 Jul 13;7:15.

Insert

TSAd (SH2D2A Human)

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pSK234

Plasmid

#131149

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mKate2

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Use

Gateway destination vector

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSK247

Plasmid

#131148

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Expression

Yeast

Promoter

pSUP35, min pCYC1

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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mEos2-CD151-7

Plasmid

#57358

Purpose

Localization: Tetraspanin/Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CD151 (CD151 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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CyPet-FKBP-E2

Plasmid

#175244

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSK226

Plasmid

#129268

Purpose

dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Rnq1

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJT23_GalL_BE-PAPAP

Plasmid

#143486

Purpose

Expresses BE-PAPAP in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-PAPAP

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:FRB:nls:VPR

Plasmid

#135987

Purpose

Expresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

FRB domain fused to the VPR activation domain

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pKG3666_pGEEC576_pShip-EFS-ZF43a(inducible)-P2A-iRFP720-bGH

Plasmid

#239740

Purpose

Plasmid expressing a rapamycin-inducible transcriptional activator

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

NLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Pchi

Plasmid

#231514

Purpose

Expresses Pigoraptor chileana chimeric Sox in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Pchi HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-emerin

Plasmid

#222415

Purpose

To express emerin with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

Emerin (EMD Human)

Tags

FKBP-GFP

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL hygro-hTCAB1

Plasmid

#182593

Purpose

expresses hTCAB1 in mammalian cells

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

hTCAB1 (WRAP53 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pT2Aneo-mRFP-FKBP-mSos1-linkercat

Plasmid

#173858

Purpose

Encoding a part of the rapamycin-induced Ras activation system.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

mRFP-FKBP-mSos1-linkercat

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-0-mTagBFP2(11x7)

Plasmid

#172064

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 0 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEF HA-TSAd-CNS

Plasmid

#84558

Purpose

Mammalian expression of TSAd-CNS

Depositor

Anne Spurkland

Article

Granum et al BMC Immunol. 2006 Jul 13;7:15.

Insert

TSAd (SH2D2A Human)

Tags

HA

Expression

Mammalian

Mutation

deletion of exon 7

Promoter

EF1a

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only