We narrowed to 15,732 results for: LEA

1. pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

   Plasmid

   #228735

   Purpose

   Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R

   Plasmid

   #228731

   Purpose

   Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS2713-CMV-iNme2-ABE8e-i1-L10

   Plasmid

   #228734

   Purpose

   Expresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid iNme2 nCas9 (D16A)-i1-L10
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. BPK3082

   Plasmid

   #78742

   Purpose

   Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   LbCpf1 crRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBJG1 T7-8xHis-OGT

   Plasmid

   #154271

   Purpose

   Bacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Gross et al J Am Chem Soc. 2005 Oct 26;127(42):14588

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   bacterial codon optimized

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3_LuxSit_i_mTagBFP2

   Plasmid

   #194300

   Purpose

   Mammalian expression of the de novo luciferase, LuxSit-i

   Depositor

   David Baker, Andy Yeh

   Article

   Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

   Insert

   LuxSit-i
   

   Tags

   mTagBFP2

   Expression

   Mammalian

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-N-TEV-Twin-Strep

   Plasmid

   #202526

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Type

   Empty backbone

   Tags

   TEV cleavable site and Twin-strep-tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pY004 (pcDNA3.1-hFnCpf1)

   Plasmid

   #69976

   Purpose

   Expresses humanized FnCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hFnCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pN256

   Plasmid

   #188472

   Purpose

   scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   eGFP-WPRE-DAISY-4T
   

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRIPZ shTREX1

    Plasmid

    #127700

    Purpose

    Doxycyclin inducible shRNA knockdown of both human and mouse TREX1 gene

    Depositor

    Sandra Demaria

    Article

    Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

    Insert

    shTREX1 (Trex1 Mouse, Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pASPIre2

    Plasmid

    #154843

    Purpose

    Derivative of pASPIre1 carrying a bxb1-sfGFP translational fusion in place of bxb1

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

    Insert

    Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked discriminator containing mCherry; rhaRS operon
    

    Expression

    Bacterial

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. gRNA-ura-HYB

    Plasmid

    #64330

    Purpose

    ura3 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of ura3 deletion gRNA cassette
    

    Expression

    Yeast

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pWUR790

    Plasmid

    #101723

    Purpose

    Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

    Depositor

    John van der Oost

    Article

    Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

    Insert

    PfAgo
    

    Tags

    Strep(II)-tag

    Expression

    Bacterial

    Promoter

    T7 Promotor

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAcCas12nHS_RV6_PT1_empty

    Plasmid

    #203810

    Purpose

    Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcCas12nHS_RV6_PT1
    

    Use

    CRISPR

    Promoter

    CMV

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcdna nesprin HL

    Plasmid

    #68128

    Purpose

    nesprin headless control

    Depositor

    Daniel Conway

    Article

    Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

    Insert

    nesprin-2 (Syne2 Mouse)
    

    Expression

    Mammalian

    Mutation

    deleted actin-binding domain

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Cyclin D1

    Plasmid

    #19927

    Depositor

    Yue Xiong

    Article

    Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 9

    Insert

    Cyclin D1 (CCND1 Human)
    

    Expression

    Mammalian

    Available Since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-C-TEV-Twin-Strep

    Plasmid

    #202527

    Purpose

    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

    Plasmid

    #86419

    Purpose

    C-terminal PCR tagging module for yeast codon optimized split GFP1-10

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    GFP1-10
    

    Expression

    Yeast

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRIP-SFFV-tagBFP-2A-ICP0

    Plasmid

    #235535

    Purpose

    To express tagBFP-2A-ICP0

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    ICP0
    

    Use

    Lentiviral

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRE2-Bla(HA–RILP-FLAG)

    Plasmid

    #102424

    Purpose

    Dual tagged RILP for expression in mammalian cells.

    Depositor

    Steven Weinman

    Article

    Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    Rab interacting lysosomal protein (RILP Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    Tet-Responsive

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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