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AcrIIC1X

Plasmid

#128112

Purpose

AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrX (AcrIIC1 N3F/D15Q/A48I)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgGFP1-7SK-sgCas9

Plasmid

#87908

Purpose

multiple sgRNA

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgGFP1, sgCas9

Use

Gateway Cloning

Promoter

H1, 7SK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_129

Plasmid

#65738

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-129

Promoter

T7

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_100

Plasmid

#65735

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-100

Promoter

T7

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR4-RBPMS

Plasmid

#59387

Purpose

Entry vector with RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pJM118-CFP-LacI-NR3C2(MR)_NTD_Human

Plasmid

#249251

Purpose

For overexpression of CFP-LacI-MR-NTD

Depositor

Denes Hnisz

Article

Bielskute-Garcia et al Sci Adv. 2026 Feb 27;12(9):eadz7400. doi

Insert

CFP-LacI-MR-NTD

Expression

Mammalian

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pJM118-CFP-LacI-PGR(PR)_NTD_Human

Plasmid

#249252

Purpose

For overexpression of CFP-LacI-PR-NTD

Depositor

Denes Hnisz

Article

Bielskute-Garcia et al Sci Adv. 2026 Feb 27;12(9):eadz7400. doi

Insert

CFP-LacI-PR-NTD

Expression

Mammalian

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pJM118-CFP-LacI-NR3C1(GR)_NTD_Human

Plasmid

#249250

Purpose

For overexpression of CFP-LacI-GR-NTD

Depositor

Denes Hnisz

Article

Bielskute-Garcia et al Sci Adv. 2026 Feb 27;12(9):eadz7400. doi

Insert

CFP-LacI-GR-NTD

Expression

Mammalian

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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LLP993

Plasmid

#239915

Purpose

sgRNA-C compatible with TCTP-SynPro-01, TCTP-SynPro-17 and CaMV 35S-SynPro-11 as Level-0 part (Golden Gate cloning)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

sgRNA-C

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV eGFP

Plasmid

#206272

Purpose

ENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoter

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

eGFP

Use

Multimate/gateway entr 4

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 4 CMV mTagBFP

Plasmid

#206279

Purpose

ENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mTagBFP

Use

Multimate/gateway entr 4

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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AcrIIC1-mCherry chimera #10

Plasmid

#128113

Purpose

Expresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrIIC1-mCherry chimera #10

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1NhNABP2C

Plasmid

#128312

Purpose

Express chimeric protein hNABP1 N-terminus and hNABP2 C-terminus in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

chimeric protein hNABP1N+hNABP2C (NABP1, NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

chimeric protein hNABP1N+hNABP2C

Promoter

T7

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_120

Plasmid

#65737

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-120

Promoter

T7

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_144

Plasmid

#65739

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-144

Promoter

T7

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_20_196

Plasmid

#65741

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 20-196

Promoter

T7

Available Since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR™221_RBPMS2

Plasmid

#59389

Purpose

pDONR vector with RBPMS2

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Promoter

T7

Available Since

Feb. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223_KLRC2_WT

Plasmid

#81886

Purpose

Gateway Donor vector containing KLRC2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KLRC2 (KLRC2 Human)

Use

Gateway Cloning

Mutation

N2S; F102S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KLRC2_p.G174R

Plasmid

#81270

Purpose

Gateway Donor vector containing KLRC2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KLRC2 (KLRC2 Human)

Use

Gateway Cloning

Mutation

N2S, F102S, G174R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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DinoIII-neo

Plasmid

#155026

Purpose

Dinoflagelate backbone for amino 3'-glycosyl phosphotransferase (neo) gene

Depositor

Senjie Lin

Article

Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

Insert

amino 3'-glycosyl phosphotransferase

Expression

Bacterial

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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