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pRSFDuet-1_mNeongreen

Plasmid

#182413

Purpose

Bacterial expression of mNeongreen

Depositor

Dan Mulvihill

Publication

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen

Expression

Bacterial

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTXB1-nbOcIgG-DddA11_NT

Plasmid

#232441

Purpose

Anti-rabbit IgG Fc specific nanobody-DddA11_NT for D&Dseq (sc TFs molecular footprint)

Depositor

Ivan Raimondi

Publication

Chi et al bioRxiv [Preprint]. 2025 Jan 2:2024.12.3

Insert

nbOcIgG-DddA11_N

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPEP1

Plasmid

#61046

Purpose

BglFusion compatible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Publication

Sorg et al ACS Synth Biol. 2014 Jun 4.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTRIPZ shSTING-Mmu

Plasmid

#127697

Purpose

Doxycyclin inducible shRNA knockdown of mouse STING gene

Depositor

Sandra Demaria

Publication

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shSTING Mmu (Sting1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-CYLD-STOP

Plasmid

#60077

Purpose

N-terminal GFP tagged CYLD with stop codon, driven by CMV promoter

Depositor

Stephen Elledge

Publication

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pF148 pSOD1G37RAcGFP1

Plasmid

#26409

Depositor

Elizabeth Fisher

Publication

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G37R

Available since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles
Flag TSC2 2A(S939A + S981A)

Plasmid

#12131

Depositor

Cheryl Walker

Publication

Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

Insert

TSC2 S939A S981A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A. S981A.

Available since

July 6, 2006

Availability

Academic Institutions and Nonprofits only
819 pGEX2T PTEN C124S

Plasmid

#10745

Depositor

William Sellers

Publication

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Mutation

C124S

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pMLS7

Plasmid

#32056

Purpose

Vector optimized for constitutive gene expression in Burkholderia genus and related bacteria

Depositor

Miguel Valvano

Publication

Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

Type

Empty backbone

Expression

Bacterial

Promoter

Ps7 promoter, originated from Paraburkholderia xe…

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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H6-AP180

Plasmid

#63006

Purpose

expresses the adaptor protein AP180 in bacteria

Depositor

Ernst Ungewickell

Publication

Norris et al J Biol Chem. 1995 Jan 6;270(1):214-7.

Insert

AP180 (Snap91 Rat)

Tags

His-tag

Expression

Bacterial

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pT2KXIGdeltaIn-MCS-birA-tagRFP

Plasmid

#58378

Purpose

tol2 transgenesis for biotin based TRAP - BirA ligase

Depositor

Didier Stainier

Publication

Housley et al Development. 2014 Oct;141(20):3988-93. d

Insert

birA tagRFP

Tags

tagRFP

Available since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRSM3

Plasmid

#49799

Purpose

ePathBrick vector for pathway engineering in E. coli.

Depositor

Mattheos Koffas

Publication

Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

Type

Empty backbone

Tags

S-tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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RFP-eNOS-NLS

Plasmid

#22496

Depositor

William Sessa

Publication

Iwakiri et al Proc Natl Acad Sci U S A. 2006 Dec 26. 1

Insert

eNOS(G2A)-NLS

Tags

RFP

Expression

Mammalian

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP

Plasmid

#158654

Purpose

making transgenics expressing a QUAS driven YFP and NTR 2.0

Depositor

Jeff Mumm

Publication

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOneZeo-ORF18(WT)-3xFLAG

Plasmid

#129746

Purpose

Dox inducible expression of KSHV ORF18-3xFLAG in mammalian cells

Depositor

Britt Glaunsinger

Publication

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE3Gs and Tet inducible promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

Plasmid

#78101

Purpose

Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Publication

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-EED (EED Human, Synthetic)

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GFP KNL1 wt hardened

Plasmid

#45223

Depositor

Michael Lampson

Publication

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

GFP KNL1 wt hardened (mutated KNL1 full length that is resisted to KNL1 siRNA) (KNL1 Human)

Tags

TEV S15

Mutation

E172G

Available since

July 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
LRP6-m5-pCS2-VSVG

Plasmid

#27283

Depositor

Xi He

Publication

Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Tags

VSVG

Mutation

Changed S/T of all five PPP(S/T)P motifs to Alani…

Available since

March 3, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
2XMyc-LRRK2-RCK

Plasmid

#25065

Depositor

Mark Cookson

Publication

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the ROC-COR-Kinase domains aa 1329…

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
3xFRB-myc

Plasmid

#20226

Depositor

Carolyn Bertozzi

Publication

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFRB (MTOR Human)

Tags

myc

Expression

Mammalian

Mutation

1 FRB copy has a T2098L point mutation

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only