We narrowed to 7,814 results for: 199

1. GRK2-K220R

   Plasmid

   #35403

   Depositor

   Robert Lefkowitz

   Article

   Freedman et al J Biol Chem. 1995 Jul 28;270(30):17953-6

   Insert

   GRK2 (GRK2 Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K220R

   Promoter

   CMV

   Available since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-fNPY-GFP

   Plasmid

   #22912

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

   Insert

   fugu neuropeptide Y promoter driving GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET23b-Casp7-His

   Plasmid

   #11825

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 7 (CASP7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pBS MCK promoter

   Plasmid

   #12528

   Depositor

   Ronald Kahn

   Article

   Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

   Insert

   MCK promoter (Ckm Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS PKC theta

   Plasmid

   #8426

   Depositor

   Frederic Mushinski

   Article

   Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

   Insert

   Mouse Protein Kinase C theta (Prkcq Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 22, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pRK5 TGF beta type I receptor Flag

   Plasmid

   #14831

   Depositor

   Rik Derynck

   Article

   Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

   Insert

   TGF beta type I receptor (Tgfbr1 Rat)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBRα-σ70 D581G

   Plasmid

   #53732

   Purpose

   Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The sigma 70 moiety carries the D581G substitution

   Promoter

   lpp/lacUV5 (tandem promoter)

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

8. ECFP-GalT

   Plasmid

   #11937

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Cole et al Science. 1996 Aug 9. 273(5276):797-801.

   Insert

   GalT (GALT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amino acids 1-60 of the human galactosyltransfera…

   Promoter

   Available since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3/Myc-DNMT3A2

   Plasmid

   #36941

   Depositor

   Arthur Riggs

   Article

   Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

   Insert

   DNMT3A2 (DNMT3A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. μ2-HA-D176A

    Plasmid

    #32754

    Depositor

    Alexander Sorkin

    Article

    Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

    Insert

    μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D176A

    Promoter

    CMV

    Available since

    Nov. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pJ3 SHP2 WT

    Plasmid

    #8317

    Depositor

    Ben Neel

    Article

    Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Grp1-PH pGEX2TK

    Plasmid

    #71379

    Purpose

    bacterial expression of PH domain for phosphoinositide binding assay

    Depositor

    Mark Lemmon

    Article

    Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

    Insert

    Grp1-PH (Cyth3 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Pleckstrin homology (PH) domain; aa 264-391

    Promoter

    tac

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

13. HA-E2F-1 wt-pRcCMV

    Plasmid

    #21667

    Depositor

    William Kaelin

    Article

    Krek et al Cell. 1994 Jul 15. 78(1):161-72.

    Insert

    E2F-1 (E2F1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wild type-

    Promoter

    Available since

    Sept. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. Donor-PIGA

    Plasmid

    #153530

    Purpose

    Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    PIGA (a 1,991-bp fragment from intron 5 and exon 6)
    

    Use

    Donor plasmid

    Tags

    N/A

    Expression

    Mutation

    No mutation

    Promoter

    No promoter

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCEP4-hKCNE1

    Plasmid

    #53050

    Purpose

    Expresses beta subunit for IKs channel in mammalian cells

    Depositor

    Michael Sanguinetti

    Article

    Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

    Insert

    Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLC133

    Plasmid

    #198639

    Purpose

    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    dCas9
    

    Use

    Tags

    3x-FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. sh-RNA hAtg13

    Plasmid

    #31971

    Depositor

    Do-Hyung Kim

    Article

    Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

    Insert

    Atg13 (ATG13 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pKK233-hCaM

    Plasmid

    #47598

    Depositor

    Emanuel Strehler

    Article

    Rhyner et al Biochemistry. 1992 Dec 29;31(51):12826-3

    Insert

    CaM (CALM3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains 469 bp fragment (nt 92-559)

    Promoter

    trc

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV rat SAPAP1

    Plasmid

    #47940

    Purpose

    Expresses rat SAPAP1 in mammalian cells

    Depositor

    Yutaka Hata, Yoshimi Takai

    Article

    Takeuchi et al J Biol Chem. 1997 May 2;272(18):11943-51

    Insert

    SAPAP1 (Dlgap1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15b-Casp8 delta DED

    Plasmid

    #11827

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 8 minus Death Effecter Domain (CASP8 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Minus Death Defecter Domain (aa 1-216)

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only