We narrowed to 62,913 results for: cloning

Showing: 4841 - 4860 of 62913 results

Results per page:

Sort results by:

pLVX-opto-hCaspase-1

Plasmid

#208790

Purpose

Inducibly expresses human Caspase1, fused to light-activatable Cry2

Depositor

Petr Broz

Publication

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Caspase1 (CASP1 Human)

Use

Lentiviral

Tags

Cry2 mCherry

Expression

Mammalian

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TRE-UBC-hCD47-T2A-GFP

Plasmid

#205458

Purpose

lentiviral plasmid for expression of human CD47

Depositor

Deepta Bhattacharya

Publication

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD47 (CD47 Human)

Expression

Mammalian

Promoter

UBC

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG1159 TOM20-ECFP-FIREmate

Plasmid

#196693

Purpose

Expresses TOM20-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Publication

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

TOM20-ECFP-FIREmate

Tags

FIREmate

Expression

Mammalian

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-GFP-HRP-TM_pLX304

Plasmid

#202021

Purpose

expresses GFP-HRP anchored to the extracellular side of the plasma membrane, lentiviral vector

Depositor

Alice Ting

Publication

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

Flag-GFP-HRP-TM

Use

Lentiviral

Tags

Flag and GFP

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pM-CBE

Plasmid

#196293

Purpose

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

Depositor

Zhenghe Li

Publication

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV M antigenome encoding CBE in place of viral GP

Use

CRISPR

Tags

3×flag

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-cT3G-cERP2-NR5A1

Plasmid

#192915

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of NR5A1

Depositor

George Church

Publication

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

NR5A1 (NR5A1 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-Rab6A

Plasmid

#190171

Purpose

Expresses HaloTag-Rab6A in mammalian cells.

Depositor

Anna Akhmanova

Publication

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

HaloTag-Rab6A (RAB6A Human)

Tags

HaloTag

Expression

Mammalian

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
HaloTag-sec61b

Plasmid

#186959

Purpose

Express sec61b connected with N-terminus HaloTag

Depositor

Ke Xu

Publication

Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

Insert

Sec61 (SEC61B Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV Promoter

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MAC_N_IRF1

Plasmid

#167760

Purpose

Expresses IRF1 in mammalian cells

Depositor

Markku Varjosalo

Publication

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

IRF1 (IRF1 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-noPS

Plasmid

#177943

Purpose

Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Publication

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP

Expression

Mammalian

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED

Plasmid

#154088

Purpose

Improved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.

Depositor

George Daley

Publication

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Ligand Dependent Nuclear Receptor Corepressor, HOXA9, HOXA5 (LCOR, HOXA9, HOXA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166013

Purpose

mammalian expression of MERS-CoV soluble spike trimer protein

Depositor

Dominic Esposito

Publication

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

MERS-CoV S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOS012: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Plasmid

#163057

Purpose

Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

Depositor

Adam Cohen

Publication

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Grx1-roGFP1-iEER

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30a-Spike-E

Plasmid

#165096

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Publication

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)

Expression

Bacterial

Available since

Feb. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

Enlarge
MSCV-BCL6-t2A-BCL2

Plasmid

#135305

Purpose

Overexpression of multiple human genes (BCL2 and BCL6)

Depositor

Daniel Hodson

Publication

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

BCL6 (BCL6 Human)
BCL2 (BCL2 Human)

Use

Retroviral

Promoter

LTR

Available since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
Human J-chain

Plasmid

#158215

Purpose

Express the human J-chain

Depositor

Junyu Xiao

Publication

Li et al Science. 2020 Feb 28;367(6481):1014-1017

Insert

JCHAIN (JCHAIN Human)

Tags

8×his

Expression

Mammalian

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
RKS-Fzd7/8 subtype NGS Wnt

Plasmid

#159628

Purpose

Express Fzd7/8 subtype NGS Wnt in mammalian cells

Depositor

Chris Garcia

Publication

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7/8 subtype NGS Wnt

Use

Lentiviral

Tags

His tag

Expression

Mammalian

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge
pET-His-SCov2-nsp7

Plasmid

#154757

Purpose

Expression of SARS-CoV-2 nsp7

Depositor

Patrick Cramer

Publication

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

Enlarge
iOn-CAG∞RFP

Plasmid

#154014

Purpose

Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Publication

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

mRFP1

Expression

Mammalian

Promoter

CAG

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLPT234

Plasmid

#127855

Purpose

Repressilator 2.0, without degradation tags, integrated triple reporter

Depositor

Johan Paulsson

Publication

Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:

Inserts

bla
mSCFP3
mKate2
mVenus
TetR
LacI
cI

Use

Synthetic Biology

Expression

Bacterial

Mutation

First 11aa derived from mCherry as fusion protein

Promoter

pAmpR, pL LacO1, pL tetO1, and pR

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

Enlarge