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pHiUGE V5-epitope donor ORF2

Plasmid

#200379

Purpose

Tagging endogenously expressed proteins with V5 epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

V5-epitope

Expression

Mammalian

Mutation

NA

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pOTF3-AK (clone3)

Plasmid

#21165

Depositor

James Thomson

Article

Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

Insert

POUF5F1 (genomic DNA) (POU5F1 Human)

Use

Cre/Lox; Homologous recombination

Mutation

NeoR, EGFP, and SV40 polyA are between the two ge…

Available Since

June 25, 2009

Availability

Academic Institutions and Nonprofits only

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pCSD_1xNLS-SaCas9-1xNLS-3xHA-1xNLS

Plasmid

#107317

Purpose

Expresses SaCas9 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SaCas9

Use

CRISPR

Tags

3xHA and 2xNLS and NLS

Expression

Mammalian

Promoter

CMV IE94

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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TAL2363

Plasmid

#36776

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

EZH2-TALEN_Right (Ezh2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pSCIPpay-BFP reporter

Plasmid

#154096

Purpose

Self-Cutting and Integrating Payload CRISPR backbone with BFP reporter

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

P2A-tagBFP

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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TAL2403

Plasmid

#36806

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

ATM-TALEN_Right (Atm Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pFGL1169R

Plasmid

#116899

Purpose

CenpC:mCherry (centromere marker)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Inserts

Cenp C (partial)
mCherry
CenpC downstream region

Use

Fungal expression (in magnaporthe oryzae)

Mutation

truncated, no stop codon and without start codon

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_C3013

Plasmid

#91100

Purpose

Module C, Promoter: PvUbi2, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

GFP (+ BaeI site for GT donor cloning)

Use

Unspecified

Promoter

PvUbi2

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A3501

Plasmid

#91050

Purpose

Module A, Promoter: 35S, Gene: dAtCas9-VPR, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

dAtCas9-VPR

Use

CRISPR

Promoter

35S

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pPP384

Plasmid

#158799

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-3 (D413A)

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_NFIL3

Plasmid

#86287

Purpose

Donor vector for 3' FLAG tag of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NFIL3 homology arms (NFIL3 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIL3_2

Plasmid

#86349

Purpose

Encodes gRNA for 3' target of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against NFIL3 (NFIL3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIL3_1

Plasmid

#86348

Purpose

Encodes gRNA for 3' target of human NFIL3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against NFIL3 (NFIL3 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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TAL2236

Plasmid

#36687

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CHD4-TALEN_Left (Chd4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pAGM65893

Plasmid

#153216

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9, Bar selection cassette

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pHiUGE Myc-epitope donor ORF0

Plasmid

#200374

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pHiUGE Myc-epitope donor ORF1

Plasmid

#200375

Purpose

Tagging endogenously expressed proteins with Myc epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

Myc epitope

Expression

Mammalian

Mutation

NA

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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BPK2013-AkCas12b

Plasmid

#121950

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b

Tags

10xHis tag and 3xHA tag

Expression

Bacterial

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMG

Plasmid

#138264

Purpose

Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Inserts

eGFP flanked by iCas9 sites
mCherry

Use

CRISPR

Expression

Yeast

Promoter

Tef1

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE12

Plasmid

#79469

Purpose

sgRNA shuttle vector; module 5

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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