We narrowed to 11,805 results for: NSI;

Showing: 9721 - 9760 of 11805 results

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p-dCas9-TET1-Hygro

Plasmid

#104404

Purpose

transient expression of dCas9-TET1 fusion protein

Depositor

Stephan Beck

Insert

TET1 (TET1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHR iSH-YFP-PIF

Plasmid

#50841

Purpose

Optogenetic PI3K activation system; cytoplasmic component

Depositor

Wendell Lim

Article

Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

Inserts

p85 iSH2
PIF6 1-100

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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CMV-dCasRx-ccdB

Plasmid

#221003

Purpose

Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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Dimer 42 AA

Plasmid

#198198

Purpose

FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 42AA linker

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mVenus-42AA-mTurquoise2

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Dimer 3 AA

Plasmid

#198197

Purpose

FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 3AA linker

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mVenus-3AA-mTurquoise2

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19/N-mChe

Plasmid

#183159

Purpose

Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of mCherry.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-NFIA1.1

Plasmid

#112698

Purpose

Expression of NFIA1.1 protein for transient transfection assays

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1999 Mar 12;274(11):7072-81

Insert

Nuclear Factor I A1.1 (Nfia Mouse)

Tags

HA

Expression

Mammalian

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-cytoFLARE1.0-TEV

Plasmid

#234517

Purpose

Transcriptional reporter for detecting calcium transients in neuronal cultures (TEVp)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

EGFP-P2A-CaM-V5-TEVp

Use

AAV

Expression

Mammalian

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLCS105

Plasmid

#209358

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal Dendra2 fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLCS107

Plasmid

#209328

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mNeonGreen fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Reciever_plasmid2

Plasmid

#140695

Purpose

Encodes quorum sensing (C4-HSL) inducible cyan (sfCFP) expression

Depositor

Matthew Bennett

Article

Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

Insert

sfCFP

Expression

Bacterial

Promoter

engineered rhl promoter (responds to C4-HSL)

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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dTomato_LD 0_TEV

Plasmid

#58872

Purpose

MESA protease chain with dTomato ectodomain and no linker domain

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with dTomato ectodomain and TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-AtUBQ10:HR1b-GFP-T35S

Plasmid

#211837

Purpose

Plant transient expression cassette for HR1b-GFP fusion under UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

HR1b

Use

Synthetic Biology

Tags

GFP

Expression

Plant

Promoter

UBIQUITIN10

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pFBP_promoter_acceptor

Plasmid

#189920

Purpose

Binary vector to accept a custom promoter sequence to drive fungal luciferase expression by Agrobacterium-mediated transient transformation

Depositor

Adam Steinbrenner

Article

Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

Type

Empty backbone

Expression

Plant

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-MbPylRS-4xPyOtR-ITR-GFP

Plasmid

#240124

Purpose

Express AAV2 transgene (EGFP) and MbPylRS/tRNA for ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Pham et al bioRxiv 2024.07.20.604406

Inserts

AAV2-ITR-EGFP
MbPylRS
PylOtR

Use

AAV

Expression

Mammalian

Available Since

July 25, 2025

Availability

Academic Institutions and Nonprofits only

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pMUT2-pFF-41.5-mCherry

Plasmid

#192863

Purpose

pMUT2 based vector for sensing oxygen in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Oxygen inducible promoter

Expression

Bacterial

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pJKR-H-cdaR

Plasmid

#62557

Purpose

Glucarate biosensor. High copy plasmid that expresses GFP proportional to glucarate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Inserts

beta lactamase
sfGFP
CdaR

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19/mChe

Plasmid

#183161

Purpose

Construct for transient expression of cell nucleus-, cytoplasm-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

mCherry

Expression

Plant

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pYL322d1/N-eGFP

Plasmid

#183151

Purpose

Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pHis17_ParM _1

Plasmid

#78201

Purpose

Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

Depositor

Martin Webb

Article

Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

Insert

parM (K33A I27C T174A T175N C287A)

Tags

His6

Expression

Bacterial

Mutation

K33A I27C T174A T175N C287A

Promoter

unknown

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBK-Ma pylRS nitroY/haloY-F5

Plasmid

#212125

Purpose

Expresses Methanomethylophilus alvus pyrrolysine tRNA synthetase engineered for 3-nitroY and 3-haloY under GlnS promoter. Used as a control for Ma Pyl tRNA-synthetase selections.

Depositor

Ryan Mehl

Insert

M. alvus PylRS 3-NY/HaloY F5

Expression

Bacterial

Mutation

CTG125CTT, N166A V168S, A223C, W239R

Promoter

GlnS (constitutive)

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-URA-GFP-Ubc9ts

Plasmid

#20369

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Mutation

Y68L This mutation renders Ubc9 temperature-sens…

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pPRIME-TET-GFP-recipient

Plasmid

#11655

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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LRP6-pCS2-VSVG

Plasmid

#27282

Depositor

Xi He

Article

Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Tags

VSVG

Available Since

March 3, 2011

Availability

Academic Institutions and Nonprofits only

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pRGD-TcR

Plasmid

#74110

Purpose

Plasmid containing tetracycline resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

tetA(C)

Use

Bacterial vector based on pmb1 replicon

Mutation

C-to-T transition at pos. no. 1049 of tetA(C) lea…

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19/mChe-C

Plasmid

#183160

Purpose

Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of mCherry.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
625 pTol2-tp1bglob:egfp

Plasmid

#73586

Purpose

Tol2 vector with Notch responsive tp1 element driving expression of EGFP

Depositor

Nathan Lawson

Article

Quillien et al Development. 2014 Apr;141(7):1544-52. do

Insert

tp1 element driving EGFP

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pFH2.4_mCherry-MMLV_Psi

Plasmid

#205522

Purpose

Express mCherry-Psi (export tag consisting of MMLV Psi)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-cytoFLARE1.0/1.1-TF

Plasmid

#234516

Purpose

Transcriptional reporter for detecting calcium transients in neuronal cultures (transcription factor)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

ERT2-MK2-hLOV-TEVcs-FLAG-tTA

Use

AAV

Expression

Mammalian

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCBFRE-(mt)-luc

Plasmid

#26896

Depositor

Nicholas Gaiano

Article

Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

Insert

CBFRE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated CBF1 sites (see note)

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pCAG-b3STOPb3-ZsGreen

Plasmid

#51266

Purpose

B3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

b3STOPb3-ZsGreen

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2014

Availability

Academic Institutions and Nonprofits only

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pUC19/eCFP-C

Plasmid

#183156

Purpose

Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at C terminus of eCFP

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTR47m4

Plasmid

#104068

Purpose

Luciferase Reporter for Formaldehyde Sensing

Depositor

David Liu

Article

Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.

Inserts

xluxAB
frmR
frm Promoter m4

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Mutation

23r and 25th nucleotides in sequence mutated from…

Available Since

March 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLux76-LasI

Plasmid

#73444

Purpose

HSL relay device: produces 3O-C12-HSL in response to 3O-C6-HSL

Depositor

Jim Haseloff

Article

Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

Insert

LasI

Use

Synthetic Biology

Promoter

pLux76

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pFH2.19_mCherry-MS2x2

Plasmid

#205534

Purpose

Express mCherry-MS2x2 (export tag consisting of 2 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMUT2-pLac-mCherry

Plasmid

#192859

Purpose

pMUT2 based vector for sensing lactic acid in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

lldR operon

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FKBP_LD 0_TEV

Plasmid

#58877

Purpose

MESA protease chain with Rapamycin FKBP ectodomain and no linker domain

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGG-TurboID

Plasmid

#209391

Purpose

Transiently expressing TurboID in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

3XHA-TurboID

Expression

Plant

Mutation

TurboID gene sequence C249A

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0M-S-mNeonGreen-EC18153

Plasmid

#137075

Purpose

Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

Depositor

Julian Hibberd

Article

Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

Insert

mNeonGreen DNA synthesis product with 5' and 3' extensions for Golden Gate cloning

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
502 p5E-tp1

Plasmid

#73585

Purpose

entry vector (L4-R1) containing the Notch responsive tp1 element upstream of a basal promoter

Depositor

Nathan Lawson

Article

Quillien et al Development. 2014 Apr;141(7):1544-52. do

Insert

tp1 element

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 11,805 results for: NSI;