We narrowed to 15,732 results for: LEA

1. pcDNA-flag-mKdm4d-polyA

   Plasmid

   #61553

   Purpose

   For mRNA synthesis or expression in mammalian cells

   Depositor

   Yi Zhang

   Article

   Matoba et al Cell. 2014 Nov 6;159(4):884-95. doi: 10.

   Insert

   Kdm4d (Kdm4d Mouse)
   

   Tags

   flag

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAIP Nup62Fv2GFP

   Plasmid

   #139684

   Purpose

   Nup62 dimerization construct fused to 2 copies of GFP under an SFFV promoter

   Depositor

   Edward Campbell

   Article

   Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

   Insert

   Nup62 (NUP62 Human)
   

   Use

   Lentiviral

   Tags

   2 copies of GFP and Dimerization domain FKBP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. His-AgdNK

   Plasmid

   #203226

   Purpose

   For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
   

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-AID

   Plasmid

   #61926

   Purpose

   Expression of human AID tagged with EGFP

   Depositor

   Jon Houseley

   Article

   Lambert et al PLoS One. 2013 Dec 4;8(12):e82110. doi:

   Insert

   AICDA (AICDA Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. SQT1659

   Plasmid

   #78743

   Purpose

   Human expression plasmid for AsCpf1-NLS-3xHA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

   Insert

   mammalian codon-optimized Cpf1-NLS-3xHA
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNOC-ARS-CRISPR-v2

   Plasmid

   #99863

   Purpose

   Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Promoter

   Ribi

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCer-TG2-YFP

   Plasmid

   #85471

   Purpose

   TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

   Depositor

   Jeffrey Keillor, Ray Truant

   Article

   Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

   Insert

   hTG2 (TGM2 Human)
   

   Tags

   EYFP and mCerulean

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPBAD-yhjH

   Plasmid

   #111613

   Purpose

   Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

   Depositor

   Tim Tolker-Nielsen

   Article

   Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

   Insert

   yhjH
   

   Expression

   Bacterial

   Mutation

   A52V

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHJ423

   Plasmid

   #20681

   Depositor

   Stephen Shaw

   Article

   Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

   Insert

   Ezrin (EZR Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Changed Thr 567 to Asp

   Available Since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA6A-EGFR ECD (1-644)

    Plasmid

    #42666

    Depositor

    Mien-Chie Hung

    Article

    Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

    Insert

    EGFR ECD (1-644) (EGFR Human)
    

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFloat.cspA_v1

    Plasmid

    #133071

    Purpose

    Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

    Depositor

    David Sabatini, Kacper Rogala

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Promoter

    cspA

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. R26-EGFP HMEJ donor vector

    Plasmid

    #137927

    Purpose

    ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-EGFP-bpA with homology arms
    

    Use

    Mouse Targeting

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCer-4aa-VAMP2

    Plasmid

    #53233

    Purpose

    Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pBT311_pH11-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

    Plasmid

    #52544

    Purpose

    Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attPx3 (70 bp each)
    Neo
    PhiC31o integrase
    

    Expression

    Mammalian

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. R26-H2B-EGFP HR donor vector

    Plasmid

    #137925

    Purpose

    R0SA26 knock-in vector with homologous recombination-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-H2B-EGFP-bpA with homology arms
    

    Use

    Mouse Targeting

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ166

    Plasmid

    #109328

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1647_pGL4.51 TLV41 SSA-Luc cloning vector_Circular

    Plasmid

    #132962

    Purpose

    Firefly-luciferase single-strand annealing assay cloning backbone

    Depositor

    Gang Bao

    Article

    Cradick et al Methods Mol Biol. 2014;1114:339-52. doi:

    Insert

    Firefly Luciferase
    

    Use

    CRISPR and Luciferase

    Expression

    Mammalian

    Mutation

    Stop sequence between luciferase gene

    Promoter

    CMV

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. PAGFP_NS2B3

    Plasmid

    #195755

    Purpose

    Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

    Depositor

    Partha Mondal

    Article

    Varghese et al bioRxiv 2022.12.18.520514

    Insert

    Dengue (Type-2) NS2B3
    

    Tags

    PA-GFP

    Expression

    Mammalian

    Mutation

    No mutations

    Promoter

    CMV

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLS.405

    Plasmid

    #187203

    Purpose

    Expresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA (wildtype)
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. RPL13-ECFP-SNAP

    Plasmid

    #187967

    Purpose

    expresses RPL13 with C-terminal tags ECFP and SNAP

    Depositor

    Markus Wahl

    Article

    Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

    Insert

    RPL13 (RPL13 Human)
    

    Tags

    ECFP-SNAP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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