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Showing: 4861 - 4880 of 8781 results

  1. pEGB3_alpha1 gRNAs 2.1 P35S -104 -162 -229 (GB2759)

    Plasmid
    #160631
    Purpose
    Multiplex construct with 2.1 aptamer in scf to target -104 -162 -229 positions of 35s promoter.
    Depositor
    Diego Orzaez
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U626
    Available Since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160631-plasmid-map-sequence-id-321967

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  2. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

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  3. pCASCADE-gltA2-udhA

    Plasmid
    #65819
    Purpose
    gltA-udhA targeting guide RNA array E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA2-udhA guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induc…
    Available Since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    65819-plasmid-map-sequence-id-111979

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  4. TALEN_C18

    Plasmid
    #47974
    Purpose
    Golden Gate Compatible TALEN Construct site specific modification of genome, shortened C Terminus, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    Shortened C-Terminus,Codon Optimized FokI Endonuc…
    Promoter
    CMV
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47974-plasmid-map-sequence-id-68905

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  5. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

    Plasmid
    #135391
    Purpose
    Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.
    Depositor
    Fan Wang
    Insert
    TeLC-P2A-EYFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available Since
    Dec. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    135391-plasmid-map-sequence-id-266854

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  6. pXeDNA3.1-LacZ

    Plasmid
    #242746
    Purpose
    pXeDNA3-LacZ TA-cloning vector for dual expression: CMV enhancer/promoter in mammalian cells and Xenopus β-globin 5'/3'UTRs in oocytes; BGHpA, AmpR, NeoR, lacZα for blue-white screening.
    Depositor
    Luis Yanez-Guerra
    Type
    Empty backbone
    Use
    Xenopus oocyte expression
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    242746-plasmid-map-sequence-id-487866

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  7. pAAV-hSyn-con/fon DREADD Gq-mCherry

    Plasmid
    #200661
    Purpose
    Construct for chemogenetically activating and visualizing subpopulations of neurons in the retina
    Depositor
    Samer Hattar
    Insert
    DREADD Gq-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    200661-plasmid-map-sequence-id-393501

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  8. pDUAL CD81 (GFP)

    Plasmid
    #86980
    Purpose
    Lentiviral expression construct encoding CD81 and GFP from separate promoters
    Depositor
    Joe Grove
    Insert
    CD81 (CD81 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV/hPGK
    Available Since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    86980-plasmid-map-sequence-id-173290

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  9. AARS_C2_pNIC-Bio3

    Plasmid
    #153047
    Purpose
    Expression construct for producing Avi tagged Alanyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    AARS (AARS Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153047-plasmid-map-sequence-id-304401

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  10. pAAV-hSyn-con/fon DREADD Gi-mCherry

    Plasmid
    #200660
    Purpose
    Construct for chemogenetically silencing and visualizing subpopulations of neurons in the retina
    Depositor
    Samer Hattar
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    200660-plasmid-map-sequence-id-393500

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  11. pInt_attP1_LCS_kanR

    Plasmid
    #205300
    Purpose
    Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205300-plasmid-map-sequence-id-407004

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  12. AARS_C1_pNIC-Bio3

    Plasmid
    #153046
    Purpose
    Expression construct for producing Avi tagged Alanyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    AARS (AARS Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153046-plasmid-map-sequence-id-304398

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  15. pAGM4723:Ble:Cas9

    Plasmid
    #112208
    Purpose
    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.
    Depositor
    Thomas Mock
    Inserts
    Cas9
    SheBle
    Use
    CRISPR; Diatom, phaeodactylum
    Tags
    Phaeodactylum fcp promoter and terminator and YFP
    Mutation
    Phaeodactylum Fcp promoter
    Promoter
    Phaeodactylum Fcp
    Available Since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112208-plasmid-map-sequence-id-217248

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  16. DARS_pNIC-Bio3

    Plasmid
    #153048
    Purpose
    Expression construct for producing Avi tagged Glycyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    DARS (DARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153048-plasmid-map-sequence-id-304402

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  17. pSMART-Mev1

    Plasmid
    #65815
    Purpose
    NADPH Dependent Mevalonic Acid Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    mvaE: bifunctional acetoacetyl-CoA thiolase, and NADPH dependent HMG-CoA reductase
    mvaS: hydroxymethylglutaryl-CoA synthase
    Expression
    Bacterial
    Promoter
    insulated mipAp from E. coli and insulated waaHp…
    Available Since
    Sept. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65815-plasmid-map-sequence-id-111947

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  18. 5' EGFP SNF-5 pXOON

    Plasmid
    #48700
    Purpose
    Encodes 5' EGFP - SNF-5 fusion construct for expression in Xenopus oocytes or Mammalian Cells
    Depositor
    Dmitri Boudko
    Insert
    snf-5 (snf-5 Nematode)
    Use
    Xenopus oocyte expression
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    T7
    Available Since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    48700-plasmid-map-sequence-id-70541

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  19. pCR8GW-HA-Wnk

    Plasmid
    #203967
    Purpose
    HA-Wnk basic construct
    Depositor
    Andreas Jenny
    Insert
    Wnk (most similar to RO variant) (Wnk Fly)
    Use
    Unspecified
    Tags
    HA
    Available Since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    203967-plasmid-map-sequence-id-403297

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  20. pCASCADE-gltA1-gltA2-GapAP1

    Plasmid
    #87150
    Purpose
    gltA1-gltA2-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-gapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87150-plasmid-map-sequence-id-174939

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Showing: 4861 - 4880 of 8781 results