177,604 results
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Plasmid#108665PurposeExpression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linkerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAExpressionMammalianMutationIL-12 dimers have a Gly-Ser linkerAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pAAV-ihSyn1-tTA
Plasmid#99120PurposeAn AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTADepositorHas serviceAAV1InserttTA
UseAAVExpressionMammalianMutation*(see below)PromoterihSyn1Available sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA
Plasmid#86613PurposeVector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmidDepositorInsertATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
UseCRISPR; Co-selection via hdr using ouabainExpressionMammalianMutationK848A, K1003A, & R1060APromoterCBhAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti Lifeact-iRFP670 BlastR
Plasmid#84385PurposeLentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsiRFP670PromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hDlx-GiDREADD-dTomato-Fishell-5
Plasmid#83896PurposeGi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer elementDepositorHas serviceAAV1, AAV9, and AAV RetrogradeInserthM4Di
UseAAVExpressionMammalianPromoterhDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
Plasmid#67979PurposeCas9 activity reporter (control) with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiCas9-GFP
Plasmid#78546PurposeFusion protein Cas9+GFPDepositorInsertGFP
UseLentiviralExpressionMammalianPromoterFusion protein with Cas9Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
N174-MCS (Puro)
Plasmid#81068PurposeLentiviral vector for constitutive gene expression.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterEF-1αAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTriEx-NTOM20-LOV2
Plasmid#81009PurposeExpression of LOV2 anchored to mitochondriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLOV2
TagsNTOM20ExpressionBacterial, Insect, and Mamm…PromoterCMV, p10Available sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGAMA-YAP
Plasmid#74942PurposeLentiviral over-expression vector for YAPDepositorAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-hNox4
Plasmid#69352Purposeexpresses human Nox4 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthNOX4 (NOX4 Human)
ExpressionMammalianPromoterCMVAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_AktPH-mCherry
Plasmid#67301PurposeFluorescent indicator for PI(3,4,5)P3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAktPH-mCherry (AKT1 Synthetic, Human)
ExpressionMammalianPromoterCMVAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-EGFP-HaloTag2
Plasmid#41742DepositorInsertHalotag 2
ExpressionBacterial and MammalianPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-VSVM
Plasmid#64086PurposeExpression vector for Vesicular Stomatitis Virus Indiana strain matrix geneDepositorInsertVSV matrix protein
ExpressionMammalianPromoterCAGAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOPINB-OTULIN (full-length, aa 1-352)
Plasmid#61464PurposeExpresses human OTULIN (full-length) in E. coli.DepositorAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LBR pEGFP-N2 (646)
Plasmid#61996Purposemammalian expression of nuclear envelope transmembrane proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLBR (LBR Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-I-SceI
Plasmid#60960PurposePlasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesisDepositorInsertI-SceI restriction enzyme
Available sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LV-TRE-VP64 human MyoD-T2A-dsRedExpress2
Plasmid#60629PurposeExpresses VP64 human MyoD and dsRedExpress2 in response to doxycyclineDepositorPublicationAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Clathrin LC-15
Plasmid#55019PurposeLocalization: Clathrin Vesicles, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClathrin LC (CLTB Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mPlum-pBAD
Plasmid#54564PurposeLocalization: Protein Expression Vector , Excitation: 590, Emission: 649DepositorTypeEmpty backboneTags6xHis and mPlumExpressionBacterialAvailable sinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by