We narrowed to 11,030 results for: Kars;

1. PlayBack-Ef1a-EcoR1

   Plasmid

   #203311

   Purpose

   For expression of eukaryotic genes of interest with Ef1a promoter with EcoRI as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-Ef1a-fDIO-GCaMP6s

   Plasmid

   #105714

   Purpose

   Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.

   Depositor

   Rylan Larsen

   Has Service

   AAV8

   Insert

   GCaMP6s
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a, Human elongation factor-1 alpha

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Tet-pLKO-FOXM1-shRNA-84

   Plasmid

   #89496

   Purpose

   Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

   Depositor

   Adam Karpf

   Insert

   FOXM1 (FOXM1 Human)
   

   Use

   Lentiviral and RNAi; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BPK2014-Cas12i-Max

   Plasmid

   #188276

   Purpose

   prokaryotic expression

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   Cas12i-Max
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMDISI

   Plasmid

   #51653

   Purpose

   E coli genetic modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   spcR
   

   Use

   Genetic modification

   Tags

   Expression

   Mutation

   Promoter

   unknown

   Available Since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Tet-pLKO-FOXM1-shRNA-#3

   Plasmid

   #89500

   Purpose

   Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

   Depositor

   Adam Karpf

   Insert

   FOXM1 (FOXM1 Human)
   

   Use

   Lentiviral and RNAi; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO

   Available Since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PlayBack-CMV-EcoR1

   Plasmid

   #203305

   Purpose

   For expression of eukaryotic genes of interest with CMV promoter with EcoRI as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVX-surfaceHA-IRES-Puro

   Plasmid

   #207321

   Purpose

   Lentiviral expression of synthetic HA surface receptor in Mammalian cells

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   HAtag-Myctag-PDGFRB-TM-IRES-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha_short (EFS)

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNLS-TetR-GFP

   Plasmid

   #103838

   Purpose

   Flourescent marker for tetO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   NLS-TetR (tetR )
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   TetR: A4G (M2V)

   Promoter

   CMV

   Available Since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CamKIIa-ChrimsonR::FusionRed::Kv2.1

    Plasmid

    #102770

    Purpose

    "Activate neurons with 2p stimulation"

    Depositor

    Karel Svoboda

    Article

    Daie et al Nat Neurosci. 2021 Feb;24(2):259-265. do

    Insert

    ChrimsonR::FusionRed::Kv2.1
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1 traficking domain

    Expression

    Mutation

    Promoter

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-CaMKIIa-ChETA-EYFP

    Plasmid

    #26967

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

    Insert

    hChR2(E123T/H134R)-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T

    Promoter

    CaMKIIa

    Available Since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCW57-mcGAS-LL/RK-NLS/D307A

    Plasmid

    #185459

    Purpose

    Inducible nuclear cGAS with D307A mutation

    Depositor

    Shitao Li

    Article

    Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

    Insert

    cGAS
    

    Use

    Lentiviral

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    L157R, L159K, D307A

    Promoter

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. rTetR-GFP

    Plasmid

    #183916

    Purpose

    Reverse Tet repressor labeled with GFP; binds tetO sites upon doxycycline addition

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    rTetR-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcold-6xHis-HRV3Csite-HsTRBP

    Plasmid

    #41096

    Depositor

    Phillip Zamore

    Article

    Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

    Insert

    TRBP (TARBP2 Human)
    

    Use

    Tags

    6xHis-HRV3Csite

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5/FRT/TO-TRPT1

    Plasmid

    #184244

    Purpose

    For TRPT1 expression in mammalian cells

    Depositor

    Karla Feijs

    Article

    Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

    Insert

    TRPT1 (TRPT1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST17-hTRPT1-iso1

    Plasmid

    #184239

    Purpose

    For purification of N-terminally His-tagged TRPT1 from bacteria

    Depositor

    Karla Feijs

    Article

    Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

    Insert

    TRPT1 (TRPT1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRE3G-c-fos-iRFP-B3-repRNA-A

    Plasmid

    #159926

    Purpose

    Timestamp driven by a c-Fos promoter, with RNA barcode CTG, AKA 187.

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    iRFP-Timestamp A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE

    Plasmid

    #20294

    Purpose

    Lentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Serine

    Promoter

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-CAG-hChR2_tdTomato

    Plasmid

    #28015

    Purpose

    Expresses humanized ChR2 fused to tdTomato for optogenetic activation

    Depositor

    Karel Svoboda

    Article

    Mao et al Neuron. 2011 Oct 6;72(1):111-23.

    Insert

    humanized ChR2-tdTomato
    

    Use

    AAV; Adeno associate viral

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pV1F(Spec)

    Plasmid

    #205086

    Purpose

    Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

    Depositor

    Suomeng Dong

    Article

    Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

    Insert

    AtVIP1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV10-3xFLAG-prs-XK

    Plasmid

    #184369

    Purpose

    Express human XK in Expi293 cells

    Depositor

    Karin Reinisch

    Article

    Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

    Insert

    Human XK (XK Human)
    

    Use

    Tags

    3xFLAG-PreScission cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-Ef1a-DIO SwiChRca-TS-EYFP

    Plasmid

    #55631

    Purpose

    Cre-Dependent Chloride Channel

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

    Insert

    iC1C2-TS-EYFP
    

    Use

    AAV and Cre/Lox

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128A

    Promoter

    Ef1a

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSumo-His6-SUMO-AtTPR1(1-209)

    Plasmid

    #177858

    Purpose

    Bacterial Expression of TPR1

    Depositor

    Karsten Melcher

    Article

    Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

    Insert

    TPR1 (TPR1 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLentiN 16E6no*

    Plasmid

    #37445

    Purpose

    Lentiviral expression of HPV 16E6no* in mammalian cells

    Depositor

    Karl Munger

    Article

    Spangle et al J Virol. 2012 May 2.

    Insert

    HPV 16E6no* (E6 HPV)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    V42L

    Promoter

    CMV

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

25. cSPBN-4GFP

    Plasmid

    #52487

    Purpose

    Expresses EGFP

    Depositor

    Sebastian Seung

    Article

    Wickersham et al Nat Protoc. 2010 Mar;5(3):595-606. doi:

    Insert

    EGFP
    

    Use

    Rabies virus

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRK5-SNAP-CLIP-GB1a

    Plasmid

    #125709

    Purpose

    Eukaryotic expression of a fusion protein encoding SNAP, CLIP and the GB1a subunit of the metabotropic GABA receptor

    Depositor

    Kai Johnsson

    Article

    Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

    Insert

    SNAP-CLIP-GB1a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTn7xTS-dTomato

    Plasmid

    #117391

    Purpose

    Tn7 tagging vector pTn7xTS with dTomato expression scaffold

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Insert

    dTomato
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PlayBack-Ef1a-Bamh1

    Plasmid

    #203308

    Purpose

    For expression of eukaryotic genes of interest with Ef1a promoter with Bamh1 as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.1-hNoxa1

    Plasmid

    #58531

    Purpose

    expresses human Noxa1 in mammalian cells

    Depositor

    Karl-Heinz Krause, Botond Banfi

    Article

    Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

    Insert

    NADPH oxidase activator 1 (NOXA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAGGS-PSD95-GCaMP2

    Plasmid

    #18931

    Depositor

    Karel Svoboda

    Article

    Mao et al PLoS ONE. 2008 . 3(3):e1796.

    Insert

    PSD95-GCaMP2 (Dlg4 Rat)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

31. pMDICI

    Plasmid

    #51652

    Purpose

    E coli genetic modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    CmR
    

    Use

    Genetic modification

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

    Plasmid

    #105715

    Purpose

    In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

    Depositor

    Rylan Larsen

    Has Service

    AAV5

    Insert

    synaptophysin-GCaMP6s
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PlayBack-CMV-Kpn1

    Plasmid

    #203302

    Purpose

    For expression of eukaryotic genes of interest with CMV promoter with Kpn1 as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNCMV 16E6no*

    Plasmid

    #37454

    Purpose

    Expression of HPV 16E6no* in mammalian cells

    Depositor

    Karl Munger

    Article

    Spangle et al J Virol. 2012 May 2.

    Insert

    HPV 16E6no* (E6 HPV)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    V42L

    Promoter

    CMV

    Available Since

    Sept. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pDEST17-TARG1

    Plasmid

    #172594

    Purpose

    For purification (from E.coli) of His-tagged TARG1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    TARG1 (OARD1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGEX-4T-3-SH3BP1

    Plasmid

    #136647

    Purpose

    Expression of SHBP1-GST fusion protein in E. coli

    Depositor

    Maria Balda, Karl Matter

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1 (SH3BP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTn7xTS-sfGFP

    Plasmid

    #117390

    Purpose

    Tn7 tagging vector pTn7xTS with sfGFP expression scaffold

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-Y-RAN-GFP26

    Plasmid

    #106423

    Purpose

    Y-RAN-GFP26 (RANbody GFP26::Chicken IgY)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    Y-RAN-GFP26
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSUMO His6 SUMO AMPKa (11-281)

    Plasmid

    #177851

    Purpose

    Bacterial Expression of AMPKa1

    Depositor

    Karsten Melcher

    Article

    Li et al Cell Res. 2015 Jan;25(1):50-66. doi: 10.

    Insert

    AMPK a1 (PRKAA1 Human)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEBG-NHERF2

    Plasmid

    #28292

    Depositor

    Maria-Magdalena Georgescu

    Article

    Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

    Insert

    NHERF2 (NHERF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only