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pGEM-SNAP-TRIM21

Plasmid

#196181

Purpose

For in vitro transcription of SNAP-TRIM21 mRNA

Depositor

Binyam Mogessie

Article

Dunkley et al Sci Adv. 2023 Jan 20;9(3):eadc9161. doi:

Insert

SNAP-TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Promoter

T7

Available Since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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AiP12068 - pAAV-DLX2.0-minBG-ChrimsonR-tdTomato-WPRE3-BGHpA (Alias: CN2068)

Plasmid

#229775

Purpose

enhancer AAV for expression of ChrimsonR-tdTomato under the DLX2.0 enhancer

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Matveev et al bioRxiv 2024.11.01.621418

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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eNme2C-CBE6a

Plasmid

#215829

Purpose

Express CBE6a (with eNme2-C variant) in mammalian cells

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6a-eNme2C-2xUGI

Expression

Mammalian

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEF/myc/ER FLII12Pglu-700uDelta6

Plasmid

#17867

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLII12Pglu-700uDelta6

Tags

Citrine

Expression

Mammalian

Mutation

700uDelta6, ECFP insert in mglB

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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pTYF-mCMV/SYN-EGFP

Plasmid

#19975

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

mCMV/SYN-EGFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pBS_hNFATc4nuc

Plasmid

#24586

Depositor

Jerry Crabtree

Article

Graef et al Nature. 1999 Oct 14. 401(6754):703-8.

Insert

NFATc4 (NFATC4 Human)

Use

Cloning vector

Tags

EE tag

Mutation

S167A, S169A, S172A, S173A, S174A, S213A, S217A, …

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pRLuc110UGA

Plasmid

#41729

Depositor

James Inglese

Article

Auld et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

RLuc110UGA

Use

Luciferase

Expression

Mammalian

Mutation

in-frame UGA stop codon at codon 110 in the open …

Promoter

CMV

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pJB007

Plasmid

#98995

Purpose

Express FtsI tagged with tagRFP-t in E coli

Depositor

Jie Xiao

Article

Yang et al Science. 2017 Feb 17;355(6326):744-747.

Insert

FtsI

Tags

TagRFP-T

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-GFP-gfp_pJMP2768

Plasmid

#208889

Purpose

Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette, sfGFP

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-SH3BP1

Plasmid

#136647

Purpose

Expression of SHBP1-GST fusion protein in E. coli

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1 (SH3BP1 Human)

Expression

Bacterial

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAG308-His-redFAST

Plasmid

#160350

Purpose

Expresses His-tagged redFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

His-redFAST

Expression

Bacterial

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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UPG

Plasmid

#161003

Purpose

ubiquitous expression of PCP-GFP

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PCP-GFP

Expression

Plant

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSTARR-seq_fly-hsp70

Plasmid

#71500

Purpose

flySTARR-seq screening vector with hsp70 core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq screening vector

Tags

sgGFP (SuperGlo, Qbiogene, Inc)

Expression

Insect

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMR-A-6

Plasmid

#138520

Purpose

P3d

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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IVT-MCP-MMLV-RT

Plasmid

#207457

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-RT

Expression

Bacterial

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-fullef1a-mutFAKSPARK2

Plasmid

#195266

Purpose

express Tyr to Phe mutant FAKSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Article

Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

Insert

Tyr to Phe mutant FAKSPARK2 sensor

Use

Lentiviral

Expression

Mammalian

Promoter

ef1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a D832A

Plasmid

#113517

Purpose

Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

Depositor

Jennifer Doudna

Article

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Mutation

D832A

Promoter

T7

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-rSyntenin1

Plasmid

#89435

Purpose

mammalian expression of rat Syntenin1

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pG-HIV-LRT

Plasmid

#104592

Purpose

Standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

HIV-1 late reverse transcripts

Use

Cloning vector

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRLuc190UGA

Plasmid

#41047

Depositor

James Inglese

Article

Auld et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

RLuc190UGA

Use

Luciferase

Expression

Mammalian

Mutation

in-frame UGA stop codon at codon 190 in the open …

Promoter

CMV

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only