We narrowed to 165,343 results for: addgene

1. pEN_TT 3xFLAG-KIF20A

   Plasmid

   #192297

   Purpose

   Gateway entry vector for an inducible 3xFLAG-KIF20A

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   KIF20A (KIF20A Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAP2-CD4

   Plasmid

   #43916

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Insert

   CD4 (CD4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Human MAP2 promoter

   Available Since

   April 1, 2013

   Availability

   Academic Institutions and Nonprofits only

3. PLK1 G5.1 gRNA

   Plasmid

   #90834

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PLK1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   PLK1 (Guide Designation G5.1)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-PITCh-TUBB4B

   Plasmid

   #207785

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-hSyn-Gαq*-BERKY1

   Plasmid

   #158430

   Purpose

   BRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSyn

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   Lyn11-Nluc-ER/K linker-YFP-GRK2(RH)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   Sept. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. EGFP-Rab8b in pCSDEST2

   Plasmid

   #140881

   Purpose

   Expression of zebrafish GFP-tagged Rab8b in mammalian cells.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rab8b (rab8b Zebrafish)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. TAL2261

   Plasmid

   #36700

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   BMPR1A-TALEN_Right (BMPR1A Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   July 18, 2012

   Availability

   Academic Institutions and Nonprofits only

8. TAL2260

   Plasmid

   #36699

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   BMPR1A-TALEN_Left (BMPR1A Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pTol1-UAS:Chronos-tdTomato

   Plasmid

   #124232

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin Chronos fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   Chronos-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Promoter

   14xUAS-E1b

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA

    Plasmid

    #184633

    Purpose

    Expresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Aldh1a1 (Aldh1a1 Mouse)
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. FUp95GW (B7)

    Plasmid

    #74016

    Purpose

    lentiviral expression of shRNA resistant Psd95-EGFP fusion

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    Psd95 (DLG4 Rat)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pspCas9-POLI-ST2-com vector

    Plasmid

    #176234

    Purpose

    Vector plasmid for expressing spCas9-POLI fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-POLI to enhance expression. The ST2 loop of the sgRNA scaffold was replace

    Depositor

    Baisong Lu

    Article

    Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-TAP mLST8 (aka: GbL)

    Plasmid

    #12575

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

    Insert

    mLST8 (MLST8 Human)
    

    Use

    Retroviral

    Tags

    TAP

    Expression

    Mammalian

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

14. PSMD14 E6.4 gRNA

    Plasmid

    #90859

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PSMD14

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PSMD14 (Guide Designation E6.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSKA397

    Plasmid

    #80380

    Purpose

    mini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.

    Depositor

    Mustafa Khammash

    Article

    Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

    Inserts

    cat
    metE
    

    Use

    Synthetic Biology; Mini-tn7 delivery vector

    Expression

    Bacterial

    Promoter

    cat promoter and cpcG2Δ59

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYNL-N1_TUBB5

    Plasmid

    #65678

    Purpose

    Expresses beta-tubulin-YNL in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    beta-tubulin class V
    

    Use

    Luciferase

    Tags

    YNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pLL3.7 shRNA against CRTC2

    Plasmid

    #72711

    Purpose

    Lentiviral expression of shRNA against CRTC2 (3rd generation)

    Depositor

    Freddy Jeanneteau

    Article

    Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

    Insert

    CRTC2
    

    Use

    Lentiviral

    Tags

    CMV-EGFP

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

18. KIF2A D7.2 gRNA

    Plasmid

    #90720

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF2A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF2A (Guide Designation D7.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. KIF2A D8.2 gRNA

    Plasmid

    #90721

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KIF2A

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    KIF2A (Guide Designation D8.2)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. ATAD2 D7.3 gRNA

    Plasmid

    #90538

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ATAD2

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    ATAD2 (Guide Designation D7.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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