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pENTR221-EF1.Pro

Plasmid

#79488

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

EF1 Promoter (EEF1A1 Human, Synthetic)

Use

Gateway Cloning

Promoter

EF1 Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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eDrHER2

Plasmid

#183995

Purpose

Optically controlled eDrHER2 expression in mammalian cells

Depositor

Dmitry Gerashchenko, Vladislav Verkhusha

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrHER2 (ERBB2 Human)

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pIRES:mTREK-1(G137I)

Plasmid

#133274

Purpose

Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tag

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

G137I

Promoter

CMVd2

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-GAP43-ECFP-stop-L2

Plasmid

#186356

Purpose

Entry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequences

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Growth-associated protein-43 (Gap43 Mouse, Synthetic)

Use

Expression of a fluorescent membrane marker

Tags

ECFP

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 1 CMV EYFP Tubulin

Plasmid

#206254

Purpose

ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes EYFP Tubulin under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

EYFP Tubulin

Use

Multimate/gateway entr 1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 2 CMV mTFP1 Actin

Plasmid

#206255

Purpose

ENTR Vector 2 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTFP1 Actin under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mTFP1 Actin

Use

Multimate/gateway entr 2

Tags

mTFP1

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 3 CMV mito mCherry

Plasmid

#206256

Purpose

ENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mito mCherry

Use

Multimate/gateway entr 3

Tags

COX8 mitochondrial tag

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3-K51A

Plasmid

#155265

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

pEGFP

Expression

Mammalian

Mutation

Lysine 51 to Alanine

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3-R70A

Plasmid

#155264

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

David Rubinsztein

Article

Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

pEGFP

Expression

Mammalian

Mutation

Arginine 70 to Alanine

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-DCRD-YFP

Plasmid

#87450

Purpose

rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

Depositor

Xiaodong Liu

Article

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

DCRD (distal C-terminal regulatory domain) (CACNA1F Human)

Tags

6xHis-T7, FKBP, and YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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CFP-PCRD-FRB

Plasmid

#87449

Purpose

rapamycin-mediated heterodimerization between PCRD and DCRD from C-terminal of L-type Ca2+ channels

Depositor

Xiaodong Liu

Article

Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

Insert

PCRD (proximal C-terminal regulatory domain) (CACNA1F Human)

Tags

6xHis-T7, CFP, and FRB

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pIRES:mTREK-1(K271Q)

Plasmid

#133273

Purpose

Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

HA

Expression

Mammalian and Yeast

Mutation

K271Q

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-His6-SUMO-Yes-SH2-AviTag

Plasmid

#214208

Purpose

Bacterial expression of SH2 domain of the Yes kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Article

Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

Insert

Yes kinase SH2 domain (YES1 Human)

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pINQ-ON10

Plasmid

#198689

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, HA tag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pINQ-NbH4

Plasmid

#198690

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody H4

Tags

6xHis tag, Avitag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PCi

Plasmid

#214038

Purpose

Bacterial expression plasmid for strep-tagged Pci from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCi

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PC-σ

Plasmid

#214037

Purpose

Bacterial expression plasmid for strep-tagged PC-σ from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PC-σ

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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Cox1-FusXTBE-RightTALE

Plasmid

#196503

Purpose

Expresses right-side TALE–DddAtox C term half cox1-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox1 right TALE-G1397 DddA-C-UGI-SV40 polyA

Expression

Bacterial

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Cox3-FusXTBE-RightTALE

Plasmid

#196505

Purpose

Expresses right-side TALE–DddAtox C term half cox3-FusXTBE in zebrafish

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

IDH2 MTS-cox3 right TALE-G1397 DddA-C-UGI-SV40 polyA

Expression

Bacterial

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pICSL50009

Plasmid

#50309

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal HA tag (6x Human influenza hemagglutinin)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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