We narrowed to 10,665 results for: t7

1. pET-mKelly1

   Plasmid

   #119782

   Purpose

   Expresses mKelly1 tagged N-terminally with an MHHHHHHGSG tag

   Depositor

   Stephen Mayo

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

   Insert

   mKelly1
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Human USP30 (64-502, construct 2, MG-26-18)

   Plasmid

   #110744

   Purpose

   Bacterial expression of human USP30 (construct 2)

   Depositor

   David Komander

   Article

   Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

   Insert

   USP30 (USP30 Human)
   

   Tags

   N-His6-3C-GST-3C

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpyCatcher002-oDi

   Plasmid

   #124661

   Purpose

   Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil

   Depositor

   Mark Howarth

   Article

   Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

   Insert

   SpyCatcher002-oDi
   

   Tags

   C-tag, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMT-3P2A

   Plasmid

   #111772

   Purpose

   Quadcistronic construct: Co-express four genes by 3 P2A peptides

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Type

   Empty backbone

   Use

   Cloning intermediate

   Promoter

   T7

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57 mini-ZsGreen

   Plasmid

   #222648

   Purpose

   Vector for cloning gene of interest for in vitro transcription

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   ZsGreen
   

   Use

   In vitro transcription

   Mutation

   mutated stop codon

   Promoter

   T7

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MAP2-GCaMP3

   Plasmid

   #43917

   Depositor

   John Gearhart

   Article

   Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

   Insert

   GCaMP3
   

   Use

   Lentiviral

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   E247V in GCaMP3

   Promoter

   Human MAP2 promoter

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

   Plasmid

   #216283

   Purpose

   Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SpyCatcher003-TEVs-SpyTag003DA
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-StagRFP

   Plasmid

   #173014

   Purpose

   Rationally enhanced red fluorescent protein derived from TagRFP-T. For mammalian expression.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   StagRFP
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. CL7-LbCas12a

   Plasmid

   #164658

   Purpose

   Bacterial expression of LbCas12a using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a
   

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET29b-pduCDE

    Plasmid

    #110138

    Purpose

    Expresses B12-dependent propanediol dehydratase in E. coli BL21(DE3)

    Depositor

    Emily Balskus

    Article

    Levin et al Biochemistry. 2018 Mar 16. doi: 10.1021/

    Insert

    pduCDE (pduE, pduD, pduC Klebsiella oxytoca ATCC 8724)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Drp1-EGFP-StrepII

    Plasmid

    #174429

    Purpose

    Expresses human Drp1 Isoform 3 with EGFP at C-terminus in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Tags

    EGFP-StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpyCatcher002-oTet

    Plasmid

    #124663

    Purpose

    Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oTet
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. SUMO_MT3

    Plasmid

    #200303

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

    Depositor

    Artur Krężel

    Article

    Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

    Insert

    metallothionein 3 (MT3 Human)
    

    Tags

    Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCC25

    Plasmid

    #177319

    Purpose

    Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

    Depositor

    Rafal Tomecki

    Article

    Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

    Insert

    TtCutA 240-603
    

    Tags

    6xHis-SUMOTag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpyCatcher002-oTri

    Plasmid

    #124662

    Purpose

    Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oTri
    

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-Ec.coaD (pESC106)

    Plasmid

    #50388

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    PPAT
    

    Tags

    6xHis-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOPINS-UBE3C

    Plasmid

    #66711

    Purpose

    Expresses UBE3C HECT domain in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    KiAA0010 (UBE3C )
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET28_mouse-pro-IL1B

    Plasmid

    #183386

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL1B (Il1b Mouse)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-eDHFR

    Plasmid

    #107266

    Purpose

    mCherry-eDHFR in pIVT vector for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Akera et al Science. 2017 Nov 3;358(6363):668-672. d

    Insert

    mCherry-eDHFR (dhfr E.coli)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28_human-Caspase-4_enzymatic-domain

    Plasmid

    #183381

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-4 (AA94-377) (CASP4 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge