We narrowed to 12,214 results for: LEA
-
Plasmid#223174PurposeExpresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleusDepositorInsertRfxCas13d
UseLentiviralTagsNLS-HAAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
GAG-PEmax V7
Plasmid#232921PurposeOptimized construct for the expression of PEmax fused to GAG from fMLV. This plasmid is involved in the production of NanoScribesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAG FMLV fused to PEmax
UseCRISPRTagsNuclear export signals (x3)ExpressionMammalianAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
hzHDAC10
Plasmid#225542PurposeExpresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.DepositorInsertHistone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
TagsHis6-MBP tag with cleavable TEV siteExpressionBacterialMutationMutated A24E and D94APromoterT7Available sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)
Plasmid#208977PurposeEntry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)DepositorInsertpUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
UseCRISPRTagsBPNLSExpressionMammalianPromoterhuman U6Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_g5-HT2h
Plasmid#208709PurposeExpresses the green 5-HT sensor GRAB_g5-HT2h in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGreen fluorescent 5-HT sensor GRAB_g5-HT2h
ExpressionMammalianPromoterCMVAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-GRAB_g5-HT3.0mut
Plasmid#208712PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0mut in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGreen fluorescent 5-HT sensor GRAB_g5-HT3.0mut
ExpressionMammalianPromoterCMVAvailable sinceOct. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-plus-NLS-6xHis
Plasmid#207380PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9-plus
UseCRISPRTags6xHisExpressionBacterialMutationK848A, R1060A, amino acids 1005-1013 replaced wit…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_VP11H
Plasmid#188063PurposePROSS-designed versatile peroxidase 11HDepositorInsertPROSS-designed versatile peroxidase from Pleurotus ostreatus
TagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation38 PROSS mutations, described in publicationAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ad:2CMV_A2R2-N25K3
Plasmid#122242PurposeExpresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backboneDepositorUseAdenoviralTagsSignal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…ExpressionMammalianPromoterCMVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-shMcu-2
Plasmid#181867PurposeExpresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoterDepositorInsertmitochondrial calcium uniporter (Mcu Mouse)
UseAAV, Adenoviral, and Mouse TargetingTagshrGFPExpressionMammalianPromoterU6Available sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pECFP-histone H3
Plasmid#179698Purposevisualization of nucleus/chromosomeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHistone H3 (H3c10 Mouse)
TagsECFPExpressionMammalianAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
H2B-mScarlet- hRec8(297-506)-mNeonGreen
Plasmid#174717PurposeStable expression of fluorescent Separase cleavage sensor with human Rec8DepositorInsertRec8 (REC8 Human)
UseRetroviralTagsH2B-mScarlet and mNeonGreenExpressionMammalianMutation297-506Available sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCOLADuet-1_nsp10 (SARS-CoV-2)
Plasmid#169158PurposeTo express SARS-CoV-2 nsp10 in E. coliDepositorInsertnsp10 (ORF1ab SARS-CoV-2)
TagsMethionineExpressionBacterialMutationCodon optimised for E. coliPromoterT7Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-luxTA2-4F
Plasmid#165971PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRG706_lexO-SbfI_LexA-TF_LEU2MX
Plasmid#154818PurposeInducible expression of SbfI endonuclease in S. cerevisiaeDepositorInsertsSbfI endonuclease
LexA-ER-B112
TagsFLAG and NLSExpressionYeastPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
OSCA1.2-PP-GFP
Plasmid#135390PurposeExpresses OSCA1.2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOSCA1.2 (AT4G22120 Mustard Weed)
TagsEGFP tag connected to the gene via a precision pr…ExpressionMammalianPromoterCMVAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1mut-PA-mCherry
Plasmid#138251PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCBX1mut-PAmCherry
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TBXTA-c027
Plasmid#139764PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, Full-length, G177D (TBXT Human)
TagsAvi-tag (Biotin) and His6-GST-TEVExpressionBacterialMutationG177D; contains only amino acids S2- M435PromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046337
Plasmid#145566PurposeYeast Expression plasmid for SARS-CoV-2 nsp4DepositorInsertSARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCEP4-B41gp160
Plasmid#123247PurposeMammalian expression plasmid for Env from the B41 HIV-1 isolateDepositorInsertHIV-1 (B41) Env
TagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only