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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,114 results for: LEA

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Showing: 4901 - 4920 of 12114 results

  3. MUM1

    Plasmid
    #28273
    Depositor
    Cheryl Arrowsmith
    Insert
    MUM1 (MUM1 Human)
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains PWWP Domain (residues 405-538).
    Available since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. HECTD1

    Plasmid
    #32860
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HECTD1 (HECTD1 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 1879-1966 only
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. OTU04

    Plasmid
    #28157
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    OTU domain containing 7A (OTUD7A Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    UBA-like domain only
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. hzHDAC10

    Plasmid
    #225542
    Purpose
    Expresses histone deacetylase 10 (HDAC10) humanized catalytic domain, also known as hzHDAC10, in Escherichia coli cells.
    Depositor
    David Christianson
    Publication
    Herbst-Gervasoni et al ACS Chem Biol. 2020 Aug 21;15(8):2154-21
    Insert
    Histone Deacetylase 10 humanized catalytic domain (hdac10 Zebrafish)
    Tags
    His6-MBP tag with cleavable TEV site
    Expression
    Bacterial
    Mutation
    Mutated A24E and D94A
    Promoter
    T7
    Available since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225542-plasmid-map-sequence-id-448447

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  10. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

    Plasmid
    #208977
    Purpose
    Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Promoter
    human U6
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208977-plasmid-map-sequence-id-416230

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  14. Ad:2CMV_A2R2-N25K3

    Plasmid
    #122242
    Purpose
    Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone
    Depositor
    Corey Neu
    Publication
    Seelbinder et al Nat Biomed Eng. 2021 Dec;5(12):1500-1516
    Inserts
    C-terminal end of Nesprin-3 (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    Use
    Adenoviral
    Tags
    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    122242-plasmid-map-sequence-id-238909

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  15. pAAV-shMcu-2

    Plasmid
    #181867
    Purpose
    Expresses Mcu-targeted shRNA under control of the U6 promoter and hrGFP under control of the synthetic CAG promoter
    Depositor
    Hilmar Bading
    Publication
    Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc
    Insert
    mitochondrial calcium uniporter (Mcu Mouse)
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    hrGFP
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181867-plasmid-map-sequence-id-356033

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  19. pEF NS34a-msGFP-'S-mito

    Plasmid
    #158765
    Purpose
    Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
    Depositor
    Edwin Chapman
    Publication
    Vevea et al Elife. 2020 Jun 9;9. pii: 56469. doi: 10
    Insert
    NS3/4a protease and msGFP
    Expression
    Mammalian
    Mutation
    Flexible linkers between the protease and msGFP a…
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    158765-plasmid-map-sequence-id-313128

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Showing: 4901 - 4920 of 12114 results