We narrowed to 15,731 results for: LEA

1. pROSA26-promoter

   Plasmid

   #21710

   Depositor

   Philippe Soriano

   Article

   Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

   Insert

   ROSA26 promoter (Gt(ROSA)26Sor Mouse)
   

   Expression

   Mammalian

   Available Since

   July 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-Nup35-mut

   Plasmid

   #87341

   Purpose

   To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP35 (NUP35 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant changes

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDuet-1_VNp6-DARPinOFF7

   Plasmid

   #182398

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-DARPinOFF7
   

   Tags

   Vesicle Nucleating peptide 6 (VNp6)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15-His-∆NGlpG

   Plasmid

   #155131

   Purpose

   Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   insert codes for GlpG residues 87-276

   Promoter

   T7

   Available Since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZS*11_4enh

   Plasmid

   #149423

   Purpose

   Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

   Depositor

   Stan Fields

   Article

   Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

   Inserts

   rbcS-E9 enhancer
   35S core enhancer
   Cab-1 enhancer
   AB80 enhancer
   

   Tags

   T120>A

   Expression

   Bacterial

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF Synaptophysin-GCamp6s

   Plasmid

   #188981

   Purpose

   Presynaptic fluorescent Ca2+ indicator

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Synaptophysin (Syp Rat, Synthetic)
   

   Expression

   Mammalian

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2T-CAG-REV1-p2A-GFP-PuroR

   Plasmid

   #153001

   Purpose

   Overexpression of human REV1

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   REV1 (REV1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cas12j-8-puro

   Plasmid

   #194966

   Purpose

   The plasmid expresses human codon-optimized Cas12j-8 and puromycin resistence gene.

   Depositor

   Yongming Wang

   Article

   Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. RhoC FLARE.sc mCer, mVenus - wt

   Plasmid

   #65071

   Purpose

   RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus

   Promoter

   CMV, p10

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET21-pelB-LaM-3

    Plasmid

    #172769

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-3
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDR-GFPuniv

    Plasmid

    #46085

    Purpose

    In vivo recombination assay fluorescent reporter

    Depositor

    Raymond Monnat

    Article

    Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

    Insert

    DR-GFPuniv reporter
    

    Use

    Recombination reporter plasmid

    Expression

    Mammalian

    Mutation

    E5G and K163R mutations in EGFP relative to wild-…

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. FRB_LD 0_CS G_tTA-BFP

    Plasmid

    #58876

    Purpose

    MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CaMKIIa-ChRger2-TS-EYFP

    Plasmid

    #127238

    Purpose

    High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

    Insert

    ChRger2-TS-EYFP
    

    Use

    AAV

    Tags

    EYFP and SpyTag

    Expression

    Mammalian

    Promoter

    CamKIIa

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLC133

    Plasmid

    #198639

    Purpose

    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    dCas9
    

    Tags

    3x-FLAG

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pY011 (pcDNA3.1-hLb2Cpf1)

    Plasmid

    #69983

    Purpose

    Expresses humanized Lb2Cpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLb2Cpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL057

    Plasmid

    #177169

    Purpose

    Expresses a nuclease dead MRE11 in human cells

    Depositor

    Brittany Adamson

    Article

    Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

    Insert

    MRE11 H129N (MRE11 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    nuclease-inactivating mutation (H129N)

    Promoter

    EF1a

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Human NCKX3-FlagTag (pcDNA3.1-)

    Plasmid

    #75206

    Purpose

    Mammalian expression of SLC24A3

    Depositor

    Jonathan Lytton

    Article

    Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

    Insert

    SLC24A3 (SLC24A3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-nuc-Nb80

    Plasmid

    #205519

    Purpose

    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb80
    

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. R26-H2B-mCherry HR donor vector

    Plasmid

    #137928

    Purpose

    R0SA26 knock-in vector with homologous recombination-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-H2B-mCherry-bpA with homology arms
    

    Use

    Mouse Targeting

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPPEM

    Plasmid

    #162467

    Purpose

    Destination Expression vector for monocot plant primer editing

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Insert

    Cas9H840A
    

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine

    Promoter

    maize Ubi

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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