We narrowed to 29,515 results for: REP

1. pMTB2-RanGAP-Cerulean-Avi

   Plasmid

   #80068

   Purpose

   Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
   

   Use

   Zebrafish biotagging

   Tags

   Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Beta-actin2

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF5/FRT/DEST-3xHA/mGli2 P5-6D

   Plasmid

   #51254

   Purpose

   Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp

   Depositor

   Rajat Rohatgi

   Article

   Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Flpin system

   Tags

   3xHA-TEV

   Expression

   Mammalian

   Mutation

   amino acids 923,956 mutated to Asp

   Promoter

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pKLV2-U6gRNA5(Stat3)-PGKpuroBFP-W

   Plasmid

   #105027

   Purpose

   Lentiviral gRNA plasmid targeting mouse Stat3 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Stat3 (Stat3 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.2-Cx43-ML-HA

   Plasmid

   #49857

   Purpose

   Encodes human connexin 43 with all internal methionines mutated to leucine and a C_terminal HA epitope fusion tag

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   All internal Methionines mutated to Leucine. Cont…

   Promoter

   CMV

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pKI-cjACTB-EGFP-5'3'short

   Plasmid

   #129750

   Purpose

   Gene targeting in marmoset cells

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

   Insert

   ACTB (ACTB C. jacchus (common marmost))
   

   Use

   Marmoset targeting

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAWp28-v1 scaffold

   Plasmid

   #157976

   Purpose

   pBT264-U6p-{2xBbsI}-v1 sgRNA scaffold-{MfeI} Note: This plasmid is unstable. Screen multiple colonies to identify full length clones.

   Depositor

   Alan Siu Lun Wong

   Article

   Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAWp102-v2 scaffold

   Plasmid

   #157978

   Purpose

   pBT264-mutmU6p-{2xBbsI}-v2 sgRNA scaffold-{MfeI}

   Depositor

   Alan Siu Lun Wong

   Article

   Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTet-O-ATOH7-T2A-PuroR

   Plasmid

   #162343

   Purpose

   Lentiviral expression of ATOH7 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   ATOH7 (ATOH7 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST40-2XHA-Src-S4-UD-S32

   Plasmid

   #140302

   Purpose

   Expresses HA-tagged human Src-S4-UD-S32 in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src deletion mutant (SRC Human)
   

   Use

   Tags

   2X HA

   Expression

   Mammalian

   Mutation

   lacks the KD region

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST40-2XHA-Src-S4-S32-KD

    Plasmid

    #140298

    Purpose

    Expresses HA-tagged human Src-S4-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    lacks the UD region

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. SIN-CMV-Ca9-WPRE

    Plasmid

    #87874

    Purpose

    Transfer plasmid for the production of lentiviral vectors

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    nls

    Expression

    Mutation

    Human codon-optimized

    Promoter

    CMV

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR221-H1-sgGFP1-7SK-sgCas9

    Plasmid

    #87908

    Purpose

    multiple sgRNA

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgGFP1, sgCas9
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    H1, 7SK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPRIME-CMV-GFP-shDyn1

    Plasmid

    #132711

    Purpose

    Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Pdyn gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shDyn(1) (Pdyn Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. SIN-cPPT-H1-sgHTT1-PGK-mCherry-WPRE

    Plasmid

    #87885

    Purpose

    To produce lentiviral vector for HTT editing

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    shHTT1, cherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    H1, PGK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. USPQ-HTT-TALEN1

    Plasmid

    #92243

    Purpose

    TALEN targeting upstream of HTT gene (KKR), forms obligate heterodimer with USPQ-HTT-TALEN2

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. USPQ-HTT-TALEN2

    Plasmid

    #92244

    Purpose

    TALEN targeting upstream of HTT gene (ELD), forms obligate heterodimer with USPQ-HTT-TALEN1

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPRIME-CMV-GFP-shCrh1

    Plasmid

    #132709

    Purpose

    Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Crh gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shCrh(1) (Crh Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGM_Puro_shRNA n.2_mTet3

    Plasmid

    #79625

    Purpose

    Expression of shRNA for mouse Tet3

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Tet3 (Tet3 Mouse)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pAGM_Puro_shRNA n.1_mTet3

    Plasmid

    #79624

    Purpose

    Expression of shRNA for mouse Tet3

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Tet3 (Tet3 Mouse)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA8-Flag-LARS(1-360aa)

    Plasmid

    #139668

    Purpose

    Expresses N-teminal Flag tagged aa 1-360 of LARS1 protein

    Depositor

    Jie Chen

    Article

    Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

    Insert

    Leucyl-tRNA Synthetase (LARS1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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