We narrowed to 10,665 results for: t7

1. Lbpro (29-195)

   Plasmid

   #110759

   Purpose

   Bacterial expression of Lbpro

   Depositor

   Tim Skern

   Article

   Guarne et al EMBO J. 1998 Dec 15;17(24):7469-79. doi:

   Insert

   Lb pro
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SurA

   Plasmid

   #213138

   Purpose

   Expresses chaperone SurA

   Depositor

   Sebastian Hiller

   Article

   Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

   Insert

   SurA (21-428) (surA E.coli)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p5T7-LYCipi-ggpps

   Plasmid

   #122017

   Purpose

   Expresses genes for lycopene synthesis (crtI, crtB & ipi from Pantoea agglomerans, ggpps from T. canadiensis). IPTG inducible. Spec. resist.

   Depositor

   Gregory Stephanopoulos

   Article

   Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

   Insert

   GGPP synthase
   

   Expression

   Bacterial

   Mutation

   codon optimized, truncated first 98 amino acids, …

   Promoter

   T7

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET11a-link-NGFP

   Plasmid

   #52732

   Purpose

   IPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assay

   Depositor

   Lynne Regan

   Article

   Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

   Insert

   link-NGFP
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCGT-Sos Cat

   Plasmid

   #32923

   Depositor

   Dafna Bar-Sagi

   Article

   Boykevisch et al Curr Biol. 2006 Nov 7;16(21):2173-9.

   Insert

   SOS1 (SOS1 Human)
   

   Tags

   T7 epitope

   Expression

   Mammalian

   Mutation

   Contains aa 564-1049 (catalytic domain)

   Promoter

   CMV

   Available Since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

6. N-WASP

   Plasmid

   #186132

   Purpose

   For expression of recombinant N-WASp in bacteria cells.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   WASL (WASL Rat)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Amino Acid residues 183-193 fused to 273-501

   Promoter

   T7

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-Ec.coaE (pESC124)

   Plasmid

   #50390

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   DPCK
   

   Tags

   6XHIS tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. His6_MBP_FXR2P_P474S_P493N

   Plasmid

   #198695

   Purpose

   Expression of human FXR2 protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FXR2P (FXR2 Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Changed proline 474 to serine and proline 493 asp…

   Promoter

   T7

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. DCGS-10

   Plasmid

   #24752

   Depositor

   William Pavan

   Article

   Southard-Smith et al Nat Genet. 1998 Jan . 18(1):60-4.

   Insert

   (sex determining region Y)-box 10 (Sox10 Mouse)
   

   Use

   In situ probe template

   Mutation

   3' end of Sox10 cDNA for in situ probe. Tran…

   Available Since

   May 13, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pET19b+H3

    Plasmid

    #157807

    Purpose

    Expression of cysteine-free human histone H3 (c111A)

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H3
    

    Expression

    Bacterial

    Mutation

    C111A

    Promoter

    T7

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_mouse-pro-IL18

    Plasmid

    #183389

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL18 (Il18 Mouse)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTH1-SaSrtA

    Plasmid

    #64973

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    T7 promoter

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-His6-ODC-Ctag

    Plasmid

    #163614

    Purpose

    Expresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    ODC (ODC1 Human)
    

    Tags

    C-tag and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b-pchG WT

    Plasmid

    #87107

    Purpose

    expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

    Insert

    pchG WT
    

    Tags

    his-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST14-Avitag-SpyCatcher002

    Plasmid

    #102828

    Purpose

    Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    Avitag-SpyCatcher002
    

    Tags

    Avitag, GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET11d-hUba2

    Plasmid

    #53136

    Purpose

    E.coli expression of full length untagged human Uba2 (SAE2)

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    UBA2 (UBA2 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. tUI-HA

    Plasmid

    #122662

    Purpose

    Express tUI-HA protein in E. coli

    Depositor

    Robert Cohen

    Article

    Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Insert

    tUI-HA
    

    Tags

    HA-tag and His-tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 17, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pPLP4HS-mCf

    Plasmid

    #229988

    Purpose

    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Tags

    His6 and Twin-Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPLP2S-mCf

    Plasmid

    #229986

    Purpose

    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Tags

    Strep-tag II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21b-MBPx-MBPx-SpyTag003

    Plasmid

    #216291

    Purpose

    Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    MBPx-MBPx-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 and SpyTag003

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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