We narrowed to 6,927 results for: tac

Showing: 4901 - 4920 of 6927 results

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gRNA NIA Null

Plasmid

#198728

Purpose

Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AtNIA1 gRNAs

Expression

Plant

Promoter

pU6-26, pU6-29

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-rssA

Plasmid

#89957

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

rssA gRNA

Expression

Bacterial

Promoter

pTet

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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FAB(ER-LD)

Plasmid

#214424

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FRB-V5

Tags

CFAST-Halo and RspAN-FRB-V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[3xMS2_SL]

Plasmid

#68426

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing three MS2 Stem-loops

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pRDB_156

Plasmid

#216090

Purpose

Cas9 [Sp] CRISPRa targeting CD274, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD274 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCR1278

Plasmid

#111121

Purpose

Expresses XRCC2_-_152373174.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

XRCC2 gRNA (XRCC2 Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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BII-Sh-FUCCI-Luc

Plasmid

#133364

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Clover-Geminin-IRES-mKO2-Cdt1

Tags

HA-tagged Clover-Geminin

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry2-mito

Plasmid

#164164

Purpose

Expresses mCherry2-fused with a mitochondria-targeting signal of the ActA gene of Listeria monocytogenes under the control of CAG promoter

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mCherry2-mtActA

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSUPERIOR.puro-shp53

Plasmid

#38035

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Knobel et al Neoplasia. 2011 Oct;13(10):961-70.

Insert

shp53 (TP53 Human)

Use

RNAi

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSIN-Arkadia-gRNA1

Plasmid

#180374

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_833

Plasmid

#216086

Purpose

Cas9 [Sp] knockout targeting CD47, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD47 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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E407V Nuclear Myosin I YFP

Plasmid

#60242

Purpose

Expresses a E407V mutant of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding and motor activities

Depositor

Primal de Lanerolle

Article

Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

Insert

Nuclear Myosin 1 (Myo1c Mouse)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

E407V

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
MSCV-miRE_shBRD9_561-SV40-GFP

Plasmid

#75139

Purpose

Retroviral expression plasmid encoding shBRD9_561 (targeting human BRD)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBRD9_561

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mas-KB-1753-Nluc

Plasmid

#158603

Purpose

Plasmid expressing KB-1753-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pU6-Sp-gRNA-ALB_DF_B358c_attB

Plasmid

#182146

Purpose

To insert attB attachment site at ALB in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

ALB_B358c_attB pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-ALB_DF_A277c_attB

Plasmid

#182145

Purpose

To insert attB attachment site at ALB in human cells via twinPE

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

Alb_A277c_attB pegRNA

Expression

Mammalian

Promoter

hU6

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBMN-AS-HNF4α

Plasmid

#139305

Purpose

Knocking down of human HNF4α through shRNA and amiRNA targeting HNF4α

Depositor

Claes Wadelius

Article

Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

Insert

shRNA and amiRNA against HNF4α

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pU6_(GLuc)_INT[BoBS]

Plasmid

#68431

Purpose

Transient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

INT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

human U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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shLacZ

Plasmid

#42559

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

shLacZ

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pLKO mouse shRNA 2 raptor

Plasmid

#21340

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

raptor shRNA 2 (Rptor Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only