We narrowed to 4,957 results for: NOG;

1. FN(9-10, RGE)-I27-CC

   Plasmid

   #85396

   Purpose

   MTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling

   Depositor

   Khalid Salaita

   Article

   Galior et al Nano Lett. 2016 Jan 13;16(1):341-8. doi:

   Insert

   Fibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   GRGDS motif mutated to GRGES

   Promoter

   T7

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10

   Plasmid

   #162386

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8s
   

   Use

   Tags

   6xHis

   Expression

   Insect

   Mutation

   S26M F286Y Q315H

   Promoter

   hsp70

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AbVec2.0-mIgkc

   Plasmid

   #127157

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

   Depositor

   Hedda Wardemann

   Article

   Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

   Insert

   Igkc (Igkc Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. AbVec2.1-mIglc2

   Plasmid

   #127156

   Purpose

   Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant region

   Depositor

   Hedda Wardemann

   Article

   Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

   Insert

   Iglc2 (Iglc2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AbVec2.0-mIghg2b

   Plasmid

   #127155

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2b isotype

   Depositor

   Hedda Wardemann

   Article

   Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

   Insert

   Ighg2b (Ighg2b Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-dCas9-KRAB-PGK-HygR

   Plasmid

   #83890

   Purpose

   Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   humanized dead Cas9 KRAB
   aminoglycoside phosphotransferase from E. coli
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Human Ubiquitin C Promoter and mouse phosphoglyce…

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMJA289= pSin-EF2-TRAV10-P2A-TRBV25-Puro

   Plasmid

   #116876

   Purpose

   stable TCR expression in human T cells

   Depositor

   Marcos Alcocer

   Article

   Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

   Insert

   TRAV10 - P2A - TRBV25 (TRAV10, TRBV25-1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10

   Plasmid

   #162388

   Purpose

   Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8f
   

   Use

   Tags

   6xHis

   Expression

   Insect

   Mutation

   Q315L

   Promoter

   hsp70

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10

   Plasmid

   #162383

   Purpose

   LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8s
   

   Use

   Tags

   6xHis

   Expression

   Insect

   Mutation

   S26M F286Y Q315H

   Promoter

   hsp70

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8f-p10

    Plasmid

    #162385

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8f
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10

    Plasmid

    #162387

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

    Plasmid

    #162384

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Use

    Tags

    6xHis

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQCXIX-Myc-hTIM3

    Plasmid

    #110893

    Purpose

    Expression of Myc-tagged hTIM3 at the cell surface

    Depositor

    Hyeryun Choe

    Article

    Jemielity et al PLoS Pathog. 2013 Mar;9(3):e1003232. doi

    Insert

    Hepatitis A virus Cellular Receptor 2 (HAVCR2) (HAVCR2 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE2 mCherry-Rtn4HD

    Plasmid

    #86685

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4 homology domain (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    homology domain only

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE2 GFP-Rtn4HD

    Plasmid

    #86686

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4 homology domain (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    homology domain only

    Promoter

    CMV

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Islr-AP-His

    Plasmid

    #71950

    Purpose

    Expresses the entire Islr protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Islr (Islr Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFUSEss-CHIg-mG1_M18

    Plasmid

    #82357

    Purpose

    Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

    Insert

    mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    hEF1-HTLV prom

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUSEss-CHIg-mG3_M18

    Plasmid

    #82356

    Purpose

    Expression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

    Insert

    mouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    hEF1-HTLV prom

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAJE3-E7

    Plasmid

    #214359

    Purpose

    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

    Depositor

    Ryan Mehl

    Article

    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

    Inserts

    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    AmpR and lpp

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDRM18 LTN (Luciferase tdTomato Neo)

    Plasmid

    #174721

    Purpose

    Expresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.

    Depositor

    Wayne Tilley

    Article

    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

    Inserts

    Firefly Luciferase
    tdTomato
    aph
    

    Use

    Lentiviral and Luciferase

    Tags

    E2A linker and P2A linker

    Expression

    Mammalian

    Mutation

    Promoter

    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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