We narrowed to 15,565 results for: LEA

1. RhoC FLARE.sc mCer, mVenus - CA

   Plasmid

   #65424

   Purpose

   RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, Q63L in RhoC

   Promoter

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

2. RhoC FLARE.sc mCer, mVenus - DN

   Plasmid

   #65423

   Purpose

   RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, T19N in RhoC

   Promoter

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pYJ34-PB-CRISPR-BANCR-E1-gRNA

   Plasmid

   #131077

   Purpose

   long non-coding RNA BANCR knock out

   Depositor

   Xiaojun Lian

   Article

   Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

   Insert

   BANCR Exon1 gRNA (BANCR Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21 E2F1

   Plasmid

   #90313

   Purpose

   Expresses human E2F1 in bacteria

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F1 (E2F1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVH-EF1A-BRD3R

   Plasmid

   #130696

   Purpose

   Lentiviral plasmids encoding human BRD3R

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   Human BRD3R (BRD3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHS0663 pET45b(+) KraIscB-1 TwinStrep

   Plasmid

   #176536

   Purpose

   Bacterial expression of KraIscB-1-TwinStrep

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   KraIscB-1
   

   Use

   Tags

   TwinStrep

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP977

   Plasmid

   #65774

   Purpose

   Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135E mutation in Cas9

   Promoter

   CMV & T7

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV/N3IC-HA

   Plasmid

   #47618

   Purpose

   in vitro transcription and translation of HA-tagged Notch3 Intracellular domain

   Depositor

   Urban Lendahl

   Article

   Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.

   Insert

   Notch3 Intracellular domain (Notch3 Mouse)
   

   Use

   In vitro transcription and translation

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codons 1664 (Met) to 2318 (Ala)

   Promoter

   CMV

   Available since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pRSFDuet-1_VNp-LZ-mNeongreen

   Plasmid

   #182392

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-LZ-mNeongreen
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. WT (AF20)

    Plasmid

    #21359

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Promoter

    Available since

    Jan. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

    Plasmid

    #134863

    Purpose

    Expresses EGFP-tagged endoplasmic reticulum marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAMP4 (SERP1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 HA-rnSOX9 ΔC (1-381)

    Plasmid

    #62973

    Purpose

    Expresses HA-tagged rat SOX9 residues 1-383

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codes for residues 1-381

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLV U6-sgRNA UbC-tdTomato

    Plasmid

    #106949

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOCC65

    Plasmid

    #118865

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease, mGFP

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaG-26

    Plasmid

    #172751

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-26
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SuExp-mPLD3

    Plasmid

    #201245

    Purpose

    express full length mouse PLD3 protein

    Depositor

    David Nemazee

    Article

    Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

    Insert

    PLD3 (Pld3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a_Pro-IL-36γ

    Plasmid

    #214345

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL36G (IL36G Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaG-41

    Plasmid

    #172757

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-LOx-IRES-EGFP

    Plasmid

    #166877

    Purpose

    Express the bacterial lactate oxidase (LOx) enzyme in mammalian cells

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

    Insert

    Lactate oxidase
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRIPZ-Myr-PTEN

    Plasmid

    #206983

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with myristyolation signal

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    N-terminal myristoylation site

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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