We narrowed to 11,499 results for: aga

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LADL Anchor

Plasmid

#127664

Purpose

Encodes the LADL Anchor (dCas9-CIBN fusion protein) and puromycin resistance both expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

dCas9-CIBN-2A-Puro

Tags

3XFLAG

Expression

Mammalian

Mutation

Cas9 (D10A,H840A) mutant

Promoter

EF1a

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMM643

Plasmid

#112533

Purpose

pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA
luxCDABE

Expression

Bacterial

Mutation

A17P

Promoter

J23107, PL(HrtO), and ProD

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT

Plasmid

#238281

Purpose

For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2AT2A-EGFP-BRD4-NUT

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT-KS

Plasmid

#238282

Purpose

For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2AT2A-EGFP-BRD4-NUT-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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CSAC-Crys

Plasmid

#164967

Purpose

Expresses sgRNA in mammalian cells

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgCry1_2-hU6-sgCry2_1-hU6-sgCry2_2

Use

AAV

Tags

mCherry

Promoter

hU6, hSyn

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCIRCgp41-1

Plasmid

#74227

Purpose

Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

Depositor

Barbara Di Ventura, Roland Eils

Article

Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

Type

Empty backbone

Use

Synthetic Biology

Tags

gp41-1 C-intein and gp41-1 N-intein

Expression

Bacterial

Promoter

T7-lac

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDrVSP(C302S)-IRES2-EGFP

Plasmid

#80331

Purpose

Expresses Danio rerio VSP (without phosphatase activity) with separated EGFP in mammalian cell

Depositor

Yasushi Okamura

Article

Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

Insert

Daino rerio voltage-sensing phosphatase (tpte Zebrafish)

Expression

Mammalian

Mutation

C302S

Promoter

CMV

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19_msfGFP-NPM1-repair-template for U2OS

Plasmid

#238246

Purpose

For knock-in msfGFP to NPM1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1

Use

CRISPR

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX458_mCherry-NPM1-gRNA for U2OS

Plasmid

#238250

Purpose

Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to NPM1 locus

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

NPM1

Use

CRISPR

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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STARR-seq luciferase validation vector_ORI_neg.cont

Plasmid

#99315

Purpose

Negative control for luciferase validation vectors

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

neutral region; chr3: 55198740-55200041

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-PRDX1

Plasmid

#38086

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

PRDX1 peroxiredoxin 1 (PRDX1 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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SPDK3876(TRV-RNA2-pPEBV-MCS)

Plasmid

#149275

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAs

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter

Use

T-dna vector

Mutation

T2318C (DNA) in PEBV coat protein sequence

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin1.OxLight1

Plasmid

#169792

Purpose

Fluorescent reporter for orexin neuropeptides

Depositor

Tommaso Patriarchi

Article

Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

Insert

OxLight1

Use

AAV

Promoter

human Synapsin-1

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-G protein Beta2 in pcDNA3.1

Plasmid

#42182

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

G protein Beta 2 (GNB2 Human)

Tags

Venus(156-239)

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
pTBK1

Plasmid

#87443

Purpose

express TBK1 in mammalian cells

Depositor

Georgios Stathopoulos

Article

Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

Insert

TBK1 (Tbk1 Mouse)

Expression

Mammalian

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

Plasmid

#140535

Purpose

OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

mAID-EGFP-NLS TOL2

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4c/His-Xpress-HA-hBIG2(WT)

Plasmid

#79432

Purpose

Expresses N-terminally His6, Xpress and HA-tagged BIG2(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

Insert

BIG2 (ARFGEF2 Human)

Tags

HA, His6, and Xpress

Expression

Mammalian

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTN4

Plasmid

#174212

Purpose

Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Inserts

AtTIR1(F79G)::mRuby (TIR1 Nematode)
GFP

Use

CRISPR and Cre/Lox

Tags

mRuby

Expression

Worm

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-ZMYND11

Plasmid

#65402

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

ZMYND11 (ZMYND11 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only
pMV306DIG13+FFlucRT

Plasmid

#49998

Purpose

Integrase removed to increase stability of the plasmid in mycobacteria

Depositor

Brian Robertson

Article

Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

Insert

G13 promoter + Firefly luciferase Red Thermostable

Use

Mycobacteria integrating vector

Expression

Bacterial

Mutation

Codon optimized for Mycobacterium tuberculosis wi…

Promoter

G13

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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