We narrowed to 7,095 results for: mme

1. TDP-REGv2 Triple Cryptic Cre recombinase in pTwist-CMV

   Plasmid

   #216162

   Purpose

   Expresses Cre recombinase in cells lacking TDP-43 function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   Cre recombinase with N-terminal SV40 NLS and C-terminal c-Myc NLS
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. VC-Y705F+K49R-STAT3

   Plasmid

   #203721

   Purpose

   Expresses the PTM-resistant, double mutant Y705F+K49R - STAT3 fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Diallo et al Cell Oncol (Dordr). 2023 Dec 27. doi: 10

   Insert

   STAT3 tagged with VC (aa 158-238) (STAT3 Human)
   

   Use

   Tags

   VC (aa 158-238) for bimolecular fluorescence comp…

   Expression

   Mammalian

   Mutation

   Y705F+K49R substitutions

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. VC-Y705F+K685R-STAT3

   Plasmid

   #203722

   Purpose

   Expresses the PTM-resistant, double mutant Y705F+K685R - STAT3 fused to the 158-238 fragment of Venus in mammalian cells

   Depositor

   Federico Herrera

   Article

   Diallo et al Cell Oncol (Dordr). 2023 Dec 27. doi: 10

   Insert

   STAT3 tagged with VC (aa 158-238) (STAT3 Human)
   

   Use

   Tags

   VC (aa 158-238) for bimolecular fluorescence comp…

   Expression

   Mammalian

   Mutation

   Y705F+K685R substitutions

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLED.CaRPv1

   Plasmid

   #193018

   Purpose

   Excitatory/Inhibitory neuron calcium indicator (hSyn promoter, Synrg exon, jRGECO1a/jGCaMP7b)

   Depositor

   Jonathan Ling

   Article

   Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

   Insert

   Bichromatic calcium indicator (jGCaMP7b and jRGECO1a)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLED.RAB

   Plasmid

   #193015

   Purpose

   Photoreceptor-specific reporter (CBh promoter, Atp1b2 exon, bichromatic reporter)

   Depositor

   Jonathan Ling

   Article

   Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

   Insert

   Bichromatic reporter (EGFP and dsRed-Express2)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLY315

   Plasmid

   #184160

   Purpose

   Reporter circuit for hijacking mRNA of zntA as CRISPR RNA

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-zntA-1034, PrhaB, and Ptet

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLY162

   Plasmid

   #184138

   Purpose

   Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-R1, PrhaB, and Ptet

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLY163

   Plasmid

   #184141

   Purpose

   Reporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-R2, PrhaB, and Ptet

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLY168

   Plasmid

   #184142

   Purpose

   Reporter circuit for hijacking mRNA of arsenic responsive gene cluster as CRISPR RNA (site 2)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

   Inserts

   dCas9
   tetR
   pspFΔHTH::λN22plus
   sfgfp
   rhaS
   

   Use

   Synthetic Biology

   Tags

   ASV tag and λN22plus

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   Pcon, PpspA-Ar2, PrhaB, and Ptet

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLY300

    Plasmid

    #184158

    Purpose

    Reporter circuit for hijacking small RNA oxyS as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-oxyS-86, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLY320

    Plasmid

    #184161

    Purpose

    Reporter circuit for hijacking mRNA of cusCFAB as CRISPR RNA

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Inserts

    dCas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    rhaS
    

    Use

    Synthetic Biology

    Tags

    ASV tag and λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Pcon, PpspA-cusCFAB-656, PrhaB, and Ptet

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGFP-nE-mHP1alpha

    Plasmid

    #181895

    Purpose

    Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    nE-mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E

    Promoter

    CMV

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGFP-nE-mHP1alpha-delCTE

    Plasmid

    #181896

    Purpose

    Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletion

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    nE-mHP1alpha-delCTE (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S11E, S12E, S13E, S14E, 14 aa C-terminal deletion

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGFP-FUSN-mHP1alpha

    Plasmid

    #181897

    Purpose

    Fluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    FUSN-mHP1alpha (Cbx5 Mouse, Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGFP-PGL-1-mHP1alpha

    Plasmid

    #181899

    Purpose

    Fluorescently tagged HP1alpha fused to PGL-1

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    PGL-1-mHP1alpha (Cbx5 Mouse, Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRGG-GFP-RGG-mHP1alpha

    Plasmid

    #181898

    Purpose

    Fluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    RGG-GFP-RGG (Cbx5 Mouse, Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SIN40C.EFS.eGFP.mArid3a-3'UTR-Mut

    Plasmid

    #169298

    Purpose

    Constitutive overexpression a mutated 3'UTR of the murine Arid3a gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    Arid3a-3'UTR-Mut (Arid3a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Mutations in miR-125b-2 and let-7c binding sites

    Promoter

    EFS

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SIN40C.EFS.eGFP.ARID3A-3'UTR-Mut

    Plasmid

    #169296

    Purpose

    Constitutive overexpression a mutated 3'UTR of the human ARID3A gene after a GFP, to evaluate miRNA binding. Regions not containing miR-125b or let-7c binding regions not included.

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    ARID3A-3'UTR-Mut (ARID3A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Mutations in miR-125b-2 and let-7c binding sites

    Promoter

    EFS

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Δ1-60MEK1C222

    Plasmid

    #164641

    Purpose

    Bacterial expression plasmid for His6-ΔMEK1C222

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    S222C/C277S/C376S; residues 1-60 at N-terminus we…

    Promoter

    T7

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Δ1-60MEK1C218

    Plasmid

    #164643

    Purpose

    Bacterial expression plasmid for His6-ΔMEK1C218

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Use

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    S218C/C277S/C376S; residues 1-60 at N-terminus we…

    Promoter

    T7

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge